广西植物
廣西植物
엄서식물
GUIHAIA
2015年
2期
250-254,238
,共6页
王瑛华%石秋英%陈雄伟%陈刚%金红
王瑛華%石鞦英%陳雄偉%陳剛%金紅
왕영화%석추영%진웅위%진강%금홍
二花蝴蝶草%不定芽%不定根%再生植株
二花蝴蝶草%不定芽%不定根%再生植株
이화호접초%불정아%불정근%재생식주
Torenia biniflora%adventitious buds%adventitious root%regenerated plant
二花蝴蝶草(Torenia biniflora )为玄参科蝴蝶草属一年生植物,分布于广东、广西等亚热带地区,是一种观赏性较高的野生花卉.在自然生长条件下,二花蝴蝶草繁殖速度慢、增殖率低,而且花色和花型种类偏少,无法满足市场多样化的要求.植物组织培养技术为观赏植物的品种改良和新品种选育提供了新途径,目前蓝猪耳、蔓性蝴蝶草和单色蝴蝶草等蝴蝶草属植物的组织培养已获得成功,但二花蝴蝶草的组织培养尚未见有相关报道.该研究以二花蝴蝶草全展叶片为外植体,研究了培养基中添加不同种类和浓度植物生长物质对不定芽诱导和生长的影响,以及离子强度和不同浓度 IBA 对生根的影响.根据不定芽的诱导率和平均芽数筛选出最佳不定芽诱导培养基,并从生根率、平均根数和平均根长等方面筛选出最佳生根培养基.结果表明:不定芽诱导与植物生长物质的浓度和种类有关,以 MS+6-BA 0.5 mg?L-1+NAA 0.2 mg?L-1培养基的诱导效果最佳;二花蝴蝶草生根的最佳基本培养基为1/2MS,不同浓度的 IBA 对二花蝴蝶草的生根影响也不相同,其中以 IBA(0.05 mg?L-1)诱导不定芽的生根效果最佳.该研究建立了二花蝴蝶草的高频离体再生体系,为二花蝴蝶草的快速繁殖和遗传转化研究奠定了基础.
二花蝴蝶草(Torenia biniflora )為玄參科蝴蝶草屬一年生植物,分佈于廣東、廣西等亞熱帶地區,是一種觀賞性較高的野生花卉.在自然生長條件下,二花蝴蝶草繁殖速度慢、增殖率低,而且花色和花型種類偏少,無法滿足市場多樣化的要求.植物組織培養技術為觀賞植物的品種改良和新品種選育提供瞭新途徑,目前藍豬耳、蔓性蝴蝶草和單色蝴蝶草等蝴蝶草屬植物的組織培養已穫得成功,但二花蝴蝶草的組織培養尚未見有相關報道.該研究以二花蝴蝶草全展葉片為外植體,研究瞭培養基中添加不同種類和濃度植物生長物質對不定芽誘導和生長的影響,以及離子彊度和不同濃度 IBA 對生根的影響.根據不定芽的誘導率和平均芽數篩選齣最佳不定芽誘導培養基,併從生根率、平均根數和平均根長等方麵篩選齣最佳生根培養基.結果錶明:不定芽誘導與植物生長物質的濃度和種類有關,以 MS+6-BA 0.5 mg?L-1+NAA 0.2 mg?L-1培養基的誘導效果最佳;二花蝴蝶草生根的最佳基本培養基為1/2MS,不同濃度的 IBA 對二花蝴蝶草的生根影響也不相同,其中以 IBA(0.05 mg?L-1)誘導不定芽的生根效果最佳.該研究建立瞭二花蝴蝶草的高頻離體再生體繫,為二花蝴蝶草的快速繁殖和遺傳轉化研究奠定瞭基礎.
이화호접초(Torenia biniflora )위현삼과호접초속일년생식물,분포우엄동、엄서등아열대지구,시일충관상성교고적야생화훼.재자연생장조건하,이화호접초번식속도만、증식솔저,이차화색화화형충류편소,무법만족시장다양화적요구.식물조직배양기술위관상식물적품충개량화신품충선육제공료신도경,목전람저이、만성호접초화단색호접초등호접초속식물적조직배양이획득성공,단이화호접초적조직배양상미견유상관보도.해연구이이화호접초전전협편위외식체,연구료배양기중첨가불동충류화농도식물생장물질대불정아유도화생장적영향,이급리자강도화불동농도 IBA 대생근적영향.근거불정아적유도솔화평균아수사선출최가불정아유도배양기,병종생근솔、평균근수화평균근장등방면사선출최가생근배양기.결과표명:불정아유도여식물생장물질적농도화충류유관,이 MS+6-BA 0.5 mg?L-1+NAA 0.2 mg?L-1배양기적유도효과최가;이화호접초생근적최가기본배양기위1/2MS,불동농도적 IBA 대이화호접초적생근영향야불상동,기중이 IBA(0.05 mg?L-1)유도불정아적생근효과최가.해연구건립료이화호접초적고빈리체재생체계,위이화호접초적쾌속번식화유전전화연구전정료기출.
Torenia biniflora is a member of Scrophulariaceae Torenia ,and it is an annual flowering plant that widely grows in Chinese subtropical area,such as Guangdong Province and Guangxi Zhuang Autonomous Region.T .bini-flora is a wild flower with high great ornamental value.However,in natural growth condition,the reproduction speed of T .Biniflora is slow,proliferation rate is low,and the kinds of flower colour and type are few,therefore,it is una-ble to meet the diversified need of market.Plant tissue culture technology provides a new way to improve and breed new varieties for ornamental plants.Up to now,a lot of members of Torenia plants have been successfully established in vitro regeration system through tissue culture,such as T .fournieri Linden,T .balllonii Susie Wong and T .con-color Lindl.,etc.While,tissue culture for T .Biniflora has never been reported.The maln objective of this research was to establish in vitro regeneration system of T .Biniflora through tissue culture and study the effects of plant growth substances such as IBA,6-BA and NAA for clonal multiplication.In this study,we created a reliable protocol for regenerating T .biniflora plants in tissue culture by using leaf explants.The effects of different concentrations and combinations of plant growth substances on adventitious bud induction and growth were studied,and the effects of different ionic strengths of media and concentrations of IBA on rooting were analyzed.According to a series of indi-ces,such as average number of buds,frequency of bud induction,frequency of rooting,average number of root and av-erage length of root,the optimal media for inducing adventitious buds and rooting were screened respectively.The re-sults showed that adventitious bud induction was related with the concentrations and combinations of plant growth regulator,and the optimal medium for buds induction was MS+6-BA 0.5 mg?L-1 +NAA 0.2 mg?L-1 .The best medium for rooting was 1/2MS as basal medium with IBA 0.05 mg?L-1 .In this study the in vitro plant reproductive system of T .biniflora with high frequence was completely established,and it would provide an important experiment foundation for the research of rapid propagation and genetic transformation for T .biniflora .