畜牧与饲料科学
畜牧與飼料科學
축목여사료과학
ANIMAL HUSBANDRY AND FEED SCIENCE
2015年
4期
4-10,14
,共8页
孙杰%孙平%尹玉伟%韩坤%张纪亮%熊建利
孫傑%孫平%尹玉偉%韓坤%張紀亮%熊建利
손걸%손평%윤옥위%한곤%장기량%웅건리
三丁基锡%根田鼠%体重%摄食行为
三丁基錫%根田鼠%體重%攝食行為
삼정기석%근전서%체중%섭식행위
TBT%root voles%bodyweight%feeding behavior
为了探究环境浓度三丁基锡(tributyltin,TBT)对根田鼠体重及摄食相关行为的影响,实验组使用1μg/mL的TBT按5μL/(g·BW)每3 d 1次进行灌胃;对照组使用等剂量生理盐水做相同处理,实验持续45 d。记录根田鼠体重,并计算其摄食量和饮水量(供给-剩余);摄食、饮水和运动的持续时间和频次用录像记录。结果显示:TBT暴露下,雄性根田鼠的摄食频次和时间、饮水频次和时间仅在有限的几个时间点上显著高于对照组(P<0.05);对雌性根田鼠摄食和饮水的频次和时间的影响不明显。TBT暴露下,雄性根田鼠仅在灌胃第3天时其运动时间显著高于对照组(P<0.05)、静止时间显著低于对照组(P<0.05),而随着染毒时间的延长,两组之间再无显著性差异。与对照组相比,TBT暴露对雌雄根田鼠摄食量、饮水量和体重的影响均不明显。因此,环境浓度的三丁基锡对成体根田鼠的体重没有明显影响,但对其摄食相关行为有部分影响。
為瞭探究環境濃度三丁基錫(tributyltin,TBT)對根田鼠體重及攝食相關行為的影響,實驗組使用1μg/mL的TBT按5μL/(g·BW)每3 d 1次進行灌胃;對照組使用等劑量生理鹽水做相同處理,實驗持續45 d。記錄根田鼠體重,併計算其攝食量和飲水量(供給-剩餘);攝食、飲水和運動的持續時間和頻次用錄像記錄。結果顯示:TBT暴露下,雄性根田鼠的攝食頻次和時間、飲水頻次和時間僅在有限的幾箇時間點上顯著高于對照組(P<0.05);對雌性根田鼠攝食和飲水的頻次和時間的影響不明顯。TBT暴露下,雄性根田鼠僅在灌胃第3天時其運動時間顯著高于對照組(P<0.05)、靜止時間顯著低于對照組(P<0.05),而隨著染毒時間的延長,兩組之間再無顯著性差異。與對照組相比,TBT暴露對雌雄根田鼠攝食量、飲水量和體重的影響均不明顯。因此,環境濃度的三丁基錫對成體根田鼠的體重沒有明顯影響,但對其攝食相關行為有部分影響。
위료탐구배경농도삼정기석(tributyltin,TBT)대근전서체중급섭식상관행위적영향,실험조사용1μg/mL적TBT안5μL/(g·BW)매3 d 1차진행관위;대조조사용등제량생리염수주상동처리,실험지속45 d。기록근전서체중,병계산기섭식량화음수량(공급-잉여);섭식、음수화운동적지속시간화빈차용록상기록。결과현시:TBT폭로하,웅성근전서적섭식빈차화시간、음수빈차화시간부재유한적궤개시간점상현저고우대조조(P<0.05);대자성근전서섭식화음수적빈차화시간적영향불명현。TBT폭로하,웅성근전서부재관위제3천시기운동시간현저고우대조조(P<0.05)、정지시간현저저우대조조(P<0.05),이수착염독시간적연장,량조지간재무현저성차이。여대조조상비,TBT폭로대자웅근전서섭식량、음수량화체중적영향균불명현。인차,배경농도적삼정기석대성체근전서적체중몰유명현영향,단대기섭식상관행위유부분영향。
This study was conducted to investigate the effect of TBT at environment concentrations on the bodyweight and feeding related behaviors of root voles. The root voles of experimental group were administrated with 1μg/mL of TBT according to the dose of 5μL/(g·BW) once every 3 days by gavage, and those of control group were administrated with the same volume physiological saline. During the experiment period of 45 d, the body weight of the root voles was recorded and the amount of their food intake and drinking water (supply-surplus) were measured. Furthermore, the duration and frequency of feeding, drinking and movement were recorded with videotape. The results showed that only in a limited number of observation time points, the duration and frequency of feeding and drinking of male root voles in TBT exposure group were significantly higher than those in control group (P<0.05). However, there were not significant differences of the duration and frequency of feeding and drinking between female root voles in TBT exposure group and those in control group. The movement time of male root voles in the 3rd d after administrated with TBT was significantly more than that of the male root voles in control group (P<0.05), whereas the rest time was significantly less (P<0.05). With extension of the exposure time, none of significant differences of the movement and rest time were observed between the two groups. The influence of TBT exposure on the amount of food intake and drinking water and body weight of female root voles was not significant. It was indicated that the body weight of adult root voles was not significantly influenced by TBT exposure at environment concentrations, whereas the feeding related behaviors were partly influenced.