CAJ | 학술논문

为研究牛传染性鼻气管炎病毒（IBRV）感染对牛单核巨噬细胞吞噬功能的影响，应用淋巴细胞分离液获得牛外周血单个核细胞，通过贴壁培养成为单核巨噬细胞。 IBRV 感染单核巨噬细胞24 h后加入鸡红细胞，测定被感染细胞对鸡红细胞的吞噬率。结果显示，单核巨噬细胞被IBRV 感染后，对鸡红细胞的吞噬率显著降低（P＜0.05）。
위연구우전염성비기관염병독（IBRV）감염대우단핵거서세포탄서공능적영향，응용림파세포분리액획득우외주혈단개핵세포，통과첩벽배양성위단핵거서세포。 IBRV 감염단핵거서세포24 h후가입계홍세포，측정피감염세포대계홍세포적탄서솔。결과현시，단핵거서세포피IBRV 감염후，대계홍세포적탄서솔현저강저（P＜0.05）。
To investigate the effects of infectious bovine rhinotracheitis virus (IBRV) infection on phagocytizing chicken erythrocytes function of bovine monocyte-macrophage, lymphocyte separating medium was used to isolate the bovine peripheral blood mononuclear cells. The isolated cells were subsequently cultured to monocyte-macrophage by adherent culture method. The chicken erythrocytes were added to bovine monocyte-macrophage which was infected by IBRV for 24 h, and the phagocytizing chicken erythrocytes rate of the infected cells was determined. The results showed that the phagocytic chicken erythrocytes rate of IBRV infected bovine monocyte-macrophage was significantly reduced (P<0.05).