CAJ | 학술논문

目的：探讨去整合素基质金属蛋白酶（a disintegrin and metalloprotease ，ADAMs）家族成员在中国汉族人群肺癌中的表达模式。方法应用免疫组化、real‐time PCR 和 western blotting 检测 ADAM‐12、‐15和‐17在20例肺腺癌、20例肺鳞癌、10例小细胞肺癌标本以及不同病理类型肺癌细胞株中的表达。结果 ADAM‐12在肺腺癌、鳞癌以及小细胞肺癌中的阳性表达率分别为25％、20％和50％；ADAM‐15在肺腺癌、鳞癌和小细胞肺癌中的阳性表达率分别为70％、20％和10％；AD‐AM‐17在肺腺癌、鳞癌和小细胞肺癌中的阳性表达率分别为35％／、45％和10％；它们在细胞株中的表达与组织样品相一致。结论 ADAMs 家族成员在不同病理类型肺癌中的表达有较大差异，提示 ADAMs 家族成员在不同病理类型肺癌的发生、发展、浸润转移中的作用不一致。该研究为肺癌浸润转移机制的研究提供实验依据。
목적：탐토거정합소기질금속단백매（a disintegrin and metalloprotease ，ADAMs）가족성원재중국한족인군폐암중적표체모식。방법응용면역조화、real‐time PCR 화 western blotting 검측 ADAM‐12、‐15화‐17재20례폐선암、20례폐린암、10례소세포폐암표본이급불동병리류형폐암세포주중적표체。결과 ADAM‐12재폐선암、린암이급소세포폐암중적양성표체솔분별위25％、20％화50％；ADAM‐15재폐선암、린암화소세포폐암중적양성표체솔분별위70％、20％화10％；AD‐AM‐17재폐선암、린암화소세포폐암중적양성표체솔분별위35％／、45％화10％；타문재세포주중적표체여조직양품상일치。결론 ADAMs 가족성원재불동병리류형폐암중적표체유교대차이，제시 ADAMs 가족성원재불동병리류형폐암적발생、발전、침윤전이중적작용불일치。해연구위폐암침윤전이궤제적연구제공실험의거。
Objective To explore the expressions of ADAMs in Chinese Han Population lung cancer .Methods Immunohisto‐chemisty ,real‐time PCR and western blotting were employed to detect the expressions of ADAM ‐12 ,‐15 and ‐17 in lung cancer tissue samples including 20 adenocarcinoma tissues ,20 squamous tissues and 10 small cell lung cancer tissues and differential pathological lung cancer cell lines .Results The positive expression of ADAM‐12 in lung adenocarcinoma ,squamous ,and small cell lung cancer tissues was 25% ,20% and 50% .The positive expression of ADAM‐15 in differential lung cancer tissues was 70% ,20% and 10% ,and the positive expression of ADAM‐17 was 35% ,45% and 10% ,respectively .The expressions of AD‐AM‐12 ,‐15 and ‐17 in differential cell lines were consistent with the expressions in lung cancer tissues .Conclusion The expres‐sions of ADAMs in differential pathological lung cancer were significantly difference ,inferring us that the roles of ADAMs in carcinogenesis ,development ,invasion and metastasis of lung cancer were inconsistent .This study supplied experimental basis for researching the mechanism of invasion and metastasis of lung cancer .