山东大学学报(医学版)
山東大學學報(醫學版)
산동대학학보(의학판)
JOURNAL OF SHANDONG UNIVERSITY(HEALTH SCIENCES)
2015年
5期
21-26
,共6页
压力超负荷%心肌肥厚%长链非编码 RNA%长链非编码 RNA 芯片%大鼠
壓力超負荷%心肌肥厚%長鏈非編碼 RNA%長鏈非編碼 RNA 芯片%大鼠
압력초부하%심기비후%장련비편마 RNA%장련비편마 RNA 심편%대서
Myocardial hypertrophy%Pressure overload%Long noncoding RNA%LncRNA array%Rats
目的:探讨长链非编码 RNA(lncRNA)在腹主动脉缩窄术大鼠心肌肥厚和正常大鼠心肌中的差异表达。方法构建腹主动脉缩窄大鼠模型,术后4周测量大鼠超声心动图参数、左室质量指数,通过 HE 染色观察心肌细胞的面积,采用 qRT-PCR 法检测心肌肥厚相关因子 ANF、β-MHC mRNA 的表达水平,验证压力超负荷模型构建情况。采用 lncRNA 芯片技术检测 lncRNA 表达谱,筛选出具有差异性表达的 lncRNA,并通过 qRT-PCR 验证芯片结果准确性。利用 lncRNA 与 mRNA 特异性表达的标准化信号强度,构建 lncRNA 与靶基因的共表达网络。结果大鼠心肌肥厚中共检测出6969条 lncRNA,其中显著上调表达80条,显著下调表达172条。经 qRT-PCR 检测显示,在大鼠心肌肥厚中 MRAK134201下调表达,X89963上调表达,与芯片结果一致。构建共表达网络发现,与XR_008680共表达的蛋白编码基因111个。结论lncRNA 在压力超负荷性大鼠心脏组织中的表达谱发生变化,提示 lncRNA 可能在心肌肥厚的发生发展中具有一定的作用。
目的:探討長鏈非編碼 RNA(lncRNA)在腹主動脈縮窄術大鼠心肌肥厚和正常大鼠心肌中的差異錶達。方法構建腹主動脈縮窄大鼠模型,術後4週測量大鼠超聲心動圖參數、左室質量指數,通過 HE 染色觀察心肌細胞的麵積,採用 qRT-PCR 法檢測心肌肥厚相關因子 ANF、β-MHC mRNA 的錶達水平,驗證壓力超負荷模型構建情況。採用 lncRNA 芯片技術檢測 lncRNA 錶達譜,篩選齣具有差異性錶達的 lncRNA,併通過 qRT-PCR 驗證芯片結果準確性。利用 lncRNA 與 mRNA 特異性錶達的標準化信號彊度,構建 lncRNA 與靶基因的共錶達網絡。結果大鼠心肌肥厚中共檢測齣6969條 lncRNA,其中顯著上調錶達80條,顯著下調錶達172條。經 qRT-PCR 檢測顯示,在大鼠心肌肥厚中 MRAK134201下調錶達,X89963上調錶達,與芯片結果一緻。構建共錶達網絡髮現,與XR_008680共錶達的蛋白編碼基因111箇。結論lncRNA 在壓力超負荷性大鼠心髒組織中的錶達譜髮生變化,提示 lncRNA 可能在心肌肥厚的髮生髮展中具有一定的作用。
목적:탐토장련비편마 RNA(lncRNA)재복주동맥축착술대서심기비후화정상대서심기중적차이표체。방법구건복주동맥축착대서모형,술후4주측량대서초성심동도삼수、좌실질량지수,통과 HE 염색관찰심기세포적면적,채용 qRT-PCR 법검측심기비후상관인자 ANF、β-MHC mRNA 적표체수평,험증압력초부하모형구건정황。채용 lncRNA 심편기술검측 lncRNA 표체보,사선출구유차이성표체적 lncRNA,병통과 qRT-PCR 험증심편결과준학성。이용 lncRNA 여 mRNA 특이성표체적표준화신호강도,구건 lncRNA 여파기인적공표체망락。결과대서심기비후중공검측출6969조 lncRNA,기중현저상조표체80조,현저하조표체172조。경 qRT-PCR 검측현시,재대서심기비후중 MRAK134201하조표체,X89963상조표체,여심편결과일치。구건공표체망락발현,여XR_008680공표체적단백편마기인111개。결론lncRNA 재압력초부하성대서심장조직중적표체보발생변화,제시 lncRNA 가능재심기비후적발생발전중구유일정적작용。
Objective To analyze the expressions of long noncoding RNA (lncRNA)in rat myocardial hypertrophy in-duced by abdominal aortic banding and in healthy rat hearts.Methods Rat models with myocardial hypertrophy in-duced by abdominal aortic banding were established.After 4 weeks,the echocardiographic data,left ventricular mass index and the myocyte cross-section were tested.Expressions of ANF and β-MHC mRNA were determined with quanti-tative real-time polymerase chain reaction (qRT-PCR).Differences of lncRNA expression profiles were inspected with lncRNA microarray and validated with qRT-PCR.Gene co-expression network was built with normalized signal intensi-ty of specifically expressed lncRNAs and mRNAs.Results We identified 6 969 lncRNAs,among which 80 were sig-nificantly up-regulated and 172 down-regulated.The expressions of lncRNA MRAK134201 and X89963 verified by qRT-PCR and microarray data were consistent.The network constructed from lncRNA XR_008680 was co-expressed with 111 coding mRNAs.Conclusion Long noncoding RNAs are differentially expressed in rat cardiac hypertrophy models,indicating that lncRNAs might play a role in the pathogenesis of myocardial hypertrophy.