CAJ | 학술논문

目的：采用血管内超声评价短期内不同剂量阿托伐他汀对动脉粥样硬化（AS）家兔模型斑块的影响。方法30只新西兰大白兔随机分为对照组（n ＝7）、模型组（n ＝7）、阿托伐他汀低剂量组（n ＝8）和阿托伐他汀高剂量组（n ＝8）。对照组给予普通饲料喂养，其他各组均通过8周高脂喂养联合腹主动脉球囊损伤术建立 AS 模型。于第8周末行血管内超声检查。8周后，模型组，阿托伐他汀低、高剂量组继续高脂喂养，分别给予低、高剂量组兔不同剂量的阿托伐他汀干预2周。10周处死所有动物。抽取兔静脉血，测定血清血脂水平，应用 ELISA 法测定血清超敏 C 反应蛋白（hs-CRP）。并行血管内超声和病理组织学检查。结果对照组血脂水平明显低于其他各组（P ＜0．05），阿托伐他汀低、高剂量两组血脂水平和血清 hs-CRP 水平明显低于模型组（P ＜0．05），且高剂量组变化更加明显（P ＜0．01）。血管内超声显示模型组的血管外弹力膜面积（EEMA）、斑块面积（PA）、管腔面积狭窄率（LAS％）明显大于低、高剂量组（P ＜0．05），管腔面积（LA）差异无统计学意义（P ＞0．05）。结论阿托伐他汀能够短时间内降低 AS 兔的血脂水平，并减少 AS 兔斑块的形成，且与药物剂量呈正相关。
목적：채용혈관내초성평개단기내불동제량아탁벌타정대동맥죽양경화（AS）가토모형반괴적영향。방법30지신서란대백토수궤분위대조조（n ＝7）、모형조（n ＝7）、아탁벌타정저제량조（n ＝8）화아탁벌타정고제량조（n ＝8）。대조조급여보통사료위양，기타각조균통과8주고지위양연합복주동맥구낭손상술건립 AS 모형。우제8주말행혈관내초성검사。8주후，모형조，아탁벌타정저、고제량조계속고지위양，분별급여저、고제량조토불동제량적아탁벌타정간예2주。10주처사소유동물。추취토정맥혈，측정혈청혈지수평，응용 ELISA 법측정혈청초민 C 반응단백（hs-CRP）。병행혈관내초성화병리조직학검사。결과대조조혈지수평명현저우기타각조（P ＜0．05），아탁벌타정저、고제량량조혈지수평화혈청 hs-CRP 수평명현저우모형조（P ＜0．05），차고제량조변화경가명현（P ＜0．01）。혈관내초성현시모형조적혈관외탄력막면적（EEMA）、반괴면적（PA）、관강면적협착솔（LAS％）명현대우저、고제량조（P ＜0．05），관강면적（LA）차이무통계학의의（P ＞0．05）。결론아탁벌타정능구단시간내강저 AS 토적혈지수평，병감소 AS 토반괴적형성，차여약물제량정정상관。
Objective To explore the effects of different doses of atorvastatin on atherosclerotic plaques in rabbit mod-els with intravascular ultrasound.Methods A total of 30 New Zealand white rabbits were randomized into the control group (n =7 ),model group (n =7 ),low-dosage atorvastain group (n =8 )and high-dosage atorvastain group (n =8).Except for the control group,the other groups were fed with high-fat diet for 8 weeks,and aortaventralis balloon-dilation injury was created to establish AS models.Intravascular ultrasound (IVUS)was performed at the end of the 8th week.After that,the model group continued to eat high-fat diet,while the two atorvastatin groups received 2-week extra atorvastatin intervention.At the end of the 10th week,all rabbits were sacrificed.Venous blood was drawn to measure serum lipid,and the amount of inflammation mediator hs-CRP was quantified with ELISA.The abdominal aortae were detected with IVUS and then observed pathologically.Results At the end of study,serum lipid in the control group was significantly lower than that in the other three groups (P <0.05).Compared with model group,the level of serum lipid and hs-CRP decreased significantly in two atorvastatin groups,which were more significant in high-dosage atorvastain group (P <0.01).Extra-elastic membranous area (EEMA),plaque area (PA),and lumen area stenosing (LAS%)in the model group were significantly higher than those in the two atorvastatin groups (P <0.05),with no significant difference in lumen area between the model group and the two atorvastatin groups (P >0.05).Conclusion Atorvastatin is able to control the level of serum lipid observably and reduce the plaque formation in atherosclerotic rabbit models.