山东大学学报（医学版）
山東大學學報（醫學版）
산동대학학보（의학판）
JOURNAL OF SHANDONG UNIVERSITY(HEALTH SCIENCES)
2015年
5期
46-51
,共6页
涂云华%康颖倩%周英%叶振源%薛月萃%邓仁远%王梅竹%陈兰%曹煜
塗雲華%康穎倩%週英%葉振源%薛月萃%鄧仁遠%王梅竹%陳蘭%曹煜
도운화%강영천%주영%협진원%설월췌%산인원%왕매죽%진란%조욱
姜黄挥发油%THP-1 细胞%增殖%细胞凋亡%Caspase-3
薑黃揮髮油%THP-1 細胞%增殖%細胞凋亡%Caspase-3
강황휘발유%THP-1 세포%증식%세포조망%Caspase-3
Turmeric volatile oil%THP-1 cells%Proliferation%Apoptosis%Caspase-3
目的：研究姜黄挥发油（TVO）对急性单核细胞白血病 THP-1细胞株增殖及凋亡的影响，并探讨其作用机制。方法不同浓度（5～80 mg ／L）TVO 体外作用于 THP-1细胞。应用噻唑蓝（MTT）法检测细胞增殖抑制率；经吉姆萨染色，倒置显微镜观察细胞形态变化；DNA 片段化测细胞凋亡；流式细胞仪检测细胞凋亡及周期；比色法测Caspase-3酶活性。结果TVO 具有抑制 THP-1细胞株增殖的作用，并表现出时间－剂量依赖性。同时，TVO 能诱导 THP-1细胞凋亡，呈现剂量依赖性，镜下见典型细胞凋亡形态，Caspase-3酶活性随药物浓度增加而增强。经TVO 作用后的细胞周期被阻滞在 G1期，G2／M 期及 S 期细胞数减少。结论TVO 对 THP-1细胞株具有增殖抑制及促凋亡的作用，其机制可能是使细胞周期阻滞在 G1期，且激活了细胞凋亡途径中关键酶 Caspase-3活性，从而抑制肿瘤细胞的增殖与分化。
目的：研究薑黃揮髮油（TVO）對急性單覈細胞白血病 THP-1細胞株增殖及凋亡的影響，併探討其作用機製。方法不同濃度（5～80 mg ／L）TVO 體外作用于 THP-1細胞。應用噻唑藍（MTT）法檢測細胞增殖抑製率；經吉姆薩染色，倒置顯微鏡觀察細胞形態變化；DNA 片段化測細胞凋亡；流式細胞儀檢測細胞凋亡及週期；比色法測Caspase-3酶活性。結果TVO 具有抑製 THP-1細胞株增殖的作用，併錶現齣時間－劑量依賴性。同時，TVO 能誘導 THP-1細胞凋亡，呈現劑量依賴性，鏡下見典型細胞凋亡形態，Caspase-3酶活性隨藥物濃度增加而增彊。經TVO 作用後的細胞週期被阻滯在 G1期，G2／M 期及 S 期細胞數減少。結論TVO 對 THP-1細胞株具有增殖抑製及促凋亡的作用，其機製可能是使細胞週期阻滯在 G1期，且激活瞭細胞凋亡途徑中關鍵酶 Caspase-3活性，從而抑製腫瘤細胞的增殖與分化。
목적：연구강황휘발유（TVO）대급성단핵세포백혈병 THP-1세포주증식급조망적영향，병탐토기작용궤제。방법불동농도（5～80 mg ／L）TVO 체외작용우 THP-1세포。응용새서람（MTT）법검측세포증식억제솔；경길모살염색，도치현미경관찰세포형태변화；DNA 편단화측세포조망；류식세포의검측세포조망급주기；비색법측Caspase-3매활성。결과TVO 구유억제 THP-1세포주증식적작용，병표현출시간－제량의뢰성。동시，TVO 능유도 THP-1세포조망，정현제량의뢰성，경하견전형세포조망형태，Caspase-3매활성수약물농도증가이증강。경TVO 작용후적세포주기피조체재 G1기，G2／M 기급 S 기세포수감소。결론TVO 대 THP-1세포주구유증식억제급촉조망적작용，기궤제가능시사세포주기조체재 G1기，차격활료세포조망도경중관건매 Caspase-3활성，종이억제종류세포적증식여분화。
Objective To investigate the effect of turmeric volatile oil (TVO)on the apoptosis and proliferation of human leukemia cell line THP-1.Methods THP-1 Cells were incubated with different concentrations of TVO in vitro. The inhibition rate on cell proliferation was determined with methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay.The change of morphology was observed with inverted microscope after Giemsa's staining.Apoptosis was evalu-ated with DNA fragmentation and flow cytometry (FCM);cell cycle was analyzed with FCM.Caspase-3 activity was measured with a colorimetric method.Results TVO exhibited obvious inhibitive effect on the growth of THP-1 cells in a time-dose dependent manner.Meanwhile,TVO induced apoptosis of THP-1 cells in a dose-dependent manner.Typi-cal morphology changes of apoptosis were presented.Caspase-3 activity was enhanced with high concentration of TVO. After TVO treatment,the number of cells decreased in G1 /M phase and S phase but increased in G1 phase.Conclusion Turmeric volatile oil exhibits marked proliferation-inhibitive and apoptosis-inducing effect on THP-1 cells.The possi-ble mechanism is to arrest cell cycle in G1 phase,and activate the key enzyme of apoptosis pathway.
