CAJ | 학술논문

目的：探讨长期使用糖皮质激素对大鼠的骨质量影响。方法3月龄SD大鼠共38只，雌性。随机分成4组，分别是：（1）基础对照组（B组，8只，实验开始时处死，建立本批大鼠底线）；（2）正常对照组（C组，10只，腹腔注射生理盐水75 mg／kg）；（3）低剂量激素组（ L组，10只，腹腔注射甲强龙50 mg／kg）；（4）高剂量激素组（ H组，10只，腹腔注射甲强龙75 mg／kg）。实验开始时，低剂量激素组和高剂量激素组连续使用甲强龙3天（75 mg／kg?d），停用一周后，按计划使用甲强龙，每周一次，用药24周后取股骨和第三腰椎行骨生物力学实验。结果（1）与正常对照组相比，低剂量激素组和高剂量激素组的实验后体重明显减少（P＜0．05）；（2）与正常对照组相比，低剂量激素组和高剂量激素组股骨的结构力学参数（载荷、刚性）和材料力学参数（破断强度、刚性模量）均显著降低（ P＜0．05）；（3）与正常对照组相比，低剂量激素组和高剂量激素组腰椎的材料力学参数（破断强度、刚性模量）均显著降低（ P＜0．05）。结论长期使用糖皮质激素激素，无论剂量高低，均会导致大鼠股骨和腰椎骨质量下降。
목적：탐토장기사용당피질격소대대서적골질량영향。방법3월령SD대서공38지，자성。수궤분성4조，분별시：（1）기출대조조（B조，8지，실험개시시처사，건립본비대서저선）；（2）정상대조조（C조，10지，복강주사생리염수75 mg／kg）；（3）저제량격소조（ L조，10지，복강주사갑강룡50 mg／kg）；（4）고제량격소조（ H조，10지，복강주사갑강룡75 mg／kg）。실험개시시，저제량격소조화고제량격소조련속사용갑강룡3천（75 mg／kg?d），정용일주후，안계화사용갑강룡，매주일차，용약24주후취고골화제삼요추행골생물역학실험。결과（1）여정상대조조상비，저제량격소조화고제량격소조적실험후체중명현감소（P＜0．05）；（2）여정상대조조상비，저제량격소조화고제량격소조고골적결구역학삼수（재하、강성）화재료역학삼수（파단강도、강성모량）균현저강저（ P＜0．05）；（3）여정상대조조상비，저제량격소조화고제량격소조요추적재료역학삼수（파단강도、강성모량）균현저강저（ P＜0．05）。결론장기사용당피질격소격소，무론제량고저，균회도치대서고골화요추골질량하강。
Objective To determine the effect of long-term use of methylprednisolone on bone biomechanics of rats.Methods Thirty-eight 3-month-old female SD rats were chosen.They were randomly divided into basal control group ( B group, 8 rats were sacrificed at the beginning of the experiment as baseline ) , normal control group ( C group, 10 rats were given intraperitoneal injection of 75mg/kg normal saline), low dose methylprednisolone group(L group, 10 rats were given intraperitoneal injection of 50 mg/kg methylprednisolone) , and high dose methylprednisolone groug ( H group, 10 rats were given intraperitoneal injection of 75 mg/kg methylprednisolone) .Methylprednisolone was given in L group and H group for 3 days, and stopped for a week.Then the intraperitoneal injection was given once a week according to the plan.After 24 weeks of the treatment, the femurs and the lumbar spine L3 were collected for biomechanical experiment.Results (1) Compared to that in C group, the body weight in L group and H group decreased obviously after the experiment (P<0.05).(2) Compared to those in C group, structural mechanical parameters ( load and rigidity) and material mechanical parameters ( breaking strength and modulus of rigidity) of the femurs in L group and H group significantly decreased (P<0.05).(3) Compared to those in C group, structural mechanical parameters (load and rigidity) and material mechanical parameters ( breaking strength and modulus of rigidity) of the L3 in L group and H group significantly decreased ( P <0.05 ) .Conclusion The long-term use of methylprednisolone, regardless of dose, results in the decrease of bone quality of the femur and the lumbar spine of rats.