中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
9期
1654-1657
,共4页
星状神经节%大鼠%术后并发症%认知障碍%神经阻滞
星狀神經節%大鼠%術後併髮癥%認知障礙%神經阻滯
성상신경절%대서%술후병발증%인지장애%신경조체
Stellate ganglion%Rats%Postoperative complications%Cognition disorders%Nerve block
目的：评价星状神经节阻滞对老龄大鼠术后认知功能的影响以及这种影响是否与海马tau蛋白磷酸化水平有关。方法雄性SD大鼠72只。采用随机数字表法，将其随机分为3组（n=24）：正常对照组（C组）、手术组（O组）和星状神经节阻滞＋手术组（SGB组）。C、O组仅暴露右颈交感神经干但不阻滞，SGB组注射0.25%布比卡因0.15 ml行右侧SGB，给药结束后15 min时O、SGB组行剖腹探查术，手术时间30 min。C组不做任何处理。于手术后1 d（T1）、3 d（T2）、7 d（T3）每组取8只大鼠进行水迷宫实验，记录逃避潜伏期和穿越平台次数以及取海马组织，HE染色光镜下观察病理学结果以及免疫组化染色法检测海马tau蛋白磷酸化的表达水平。结果与C组比较，O、SGB组术后T1～T3时逃避潜伏期延长，穿越平台次数减少（P＜0.05），海马组织磷酸化tau蛋白表达上调（P＜0.01）。SGB组T3时逃避潜伏期,海马组织磷酸化tau蛋白表达与C组无明显差异（P＞0.05）；与O组比较，SGB组术后T1～T3时逃避潜伏期缩短，穿越平台次数增加，海马组织磷酸化tau蛋白表达下调（P＜0.05或P＜0.01）；SGB组T1～T3时海马神经元数量明显多于O组。结论星状神经节阻滞可改善老龄大鼠术后认知功能，其机制可能与抑制海马组织磷酸化tau蛋白表达有关。
目的：評價星狀神經節阻滯對老齡大鼠術後認知功能的影響以及這種影響是否與海馬tau蛋白燐痠化水平有關。方法雄性SD大鼠72隻。採用隨機數字錶法，將其隨機分為3組（n=24）：正常對照組（C組）、手術組（O組）和星狀神經節阻滯＋手術組（SGB組）。C、O組僅暴露右頸交感神經榦但不阻滯，SGB組註射0.25%佈比卡因0.15 ml行右側SGB，給藥結束後15 min時O、SGB組行剖腹探查術，手術時間30 min。C組不做任何處理。于手術後1 d（T1）、3 d（T2）、7 d（T3）每組取8隻大鼠進行水迷宮實驗，記錄逃避潛伏期和穿越平檯次數以及取海馬組織，HE染色光鏡下觀察病理學結果以及免疫組化染色法檢測海馬tau蛋白燐痠化的錶達水平。結果與C組比較，O、SGB組術後T1～T3時逃避潛伏期延長，穿越平檯次數減少（P＜0.05），海馬組織燐痠化tau蛋白錶達上調（P＜0.01）。SGB組T3時逃避潛伏期,海馬組織燐痠化tau蛋白錶達與C組無明顯差異（P＞0.05）；與O組比較，SGB組術後T1～T3時逃避潛伏期縮短，穿越平檯次數增加，海馬組織燐痠化tau蛋白錶達下調（P＜0.05或P＜0.01）；SGB組T1～T3時海馬神經元數量明顯多于O組。結論星狀神經節阻滯可改善老齡大鼠術後認知功能，其機製可能與抑製海馬組織燐痠化tau蛋白錶達有關。
목적：평개성상신경절조체대노령대서술후인지공능적영향이급저충영향시부여해마tau단백린산화수평유관。방법웅성SD대서72지。채용수궤수자표법，장기수궤분위3조（n=24）：정상대조조（C조）、수술조（O조）화성상신경절조체＋수술조（SGB조）。C、O조부폭로우경교감신경간단불조체，SGB조주사0.25%포비잡인0.15 ml행우측SGB，급약결속후15 min시O、SGB조행부복탐사술，수술시간30 min。C조불주임하처리。우수술후1 d（T1）、3 d（T2）、7 d（T3）매조취8지대서진행수미궁실험，기록도피잠복기화천월평태차수이급취해마조직，HE염색광경하관찰병이학결과이급면역조화염색법검측해마tau단백린산화적표체수평。결과여C조비교，O、SGB조술후T1～T3시도피잠복기연장，천월평태차수감소（P＜0.05），해마조직린산화tau단백표체상조（P＜0.01）。SGB조T3시도피잠복기,해마조직린산화tau단백표체여C조무명현차이（P＞0.05）；여O조비교，SGB조술후T1～T3시도피잠복기축단，천월평태차수증가，해마조직린산화tau단백표체하조（P＜0.05혹P＜0.01）；SGB조T1～T3시해마신경원수량명현다우O조。결론성상신경절조체가개선노령대서술후인지공능，기궤제가능여억제해마조직린산화tau단백표체유관。
Objective To investigate the effect of stellate ganglion block (SGB) on postoperative cognitive function and the relationship with phosphorylation levels of tau protein in aged rats. Methods Seventy-two male Sprague-Dawley rats, were randomly divided into 3 groups (n=24 each): control group (group C), operation group (group O), and SGB+ operation group (group SGB). Group C and Group O just exposed the right cervical sympathetic trunk, but not blocked. Group SGB received right SGB with 0.25% bupivacaine 0.15 ml, Groups O, SGB underwent 30 min of exploratory laparotomy starting from 15 min after the end of administration. Morris water-maze test was performed at 1 d (T1), 3 d (T2), 7 d (T3) after operation in 8 rats chosen from each group. The escape latency and frequency of crossing the original platform were recorded. Then sacrificed and the hippocampi were removed for microscopic examination and phosphorylation levels of tau protein. Results Compared with group C, the escape latency was significantly prolonged on T1-T3 after operation, the frequency of crossing the original platform was decreased (P<0.05) and the phosphorylation levels of tau protein was expressed higher in groups O and SGB. The escape latency and the phosphorylation levels of tau protein at T3 in group SGB had no significant difference with group C. Compared with group O, the escape latency was significantly shortened, the frequency of crossing the original platform was increased, and phosphorylation levels of tau protein was expressed obviously lower at T1-T3 after operation in group SGB (P<0.05 or P<0.01). The number of hippocampal neurons was significantly larger at T1-T3 in group SGB than in group O. Conclusion SGB can improve the postoperative cognitive function in aged rats, and depress over phosphorylation of tau protein may be one of its mechanisms.
