CAJ | 학술논문

目的：建立汞中毒致大鼠肾病综合征模型，为临床相关治疗手段的验证和筛选提供稳定、科学的验证平台。方法 Brown－Norway rats（ BN大鼠）雄性24只，随机分为三个不同剂量的模型组（0．5、1．0及2．0 mg／kg氯化汞溶液）及一个对照组（生理盐水组），隔天一次腹部皮下注射，并在第7、14、21、28、35天分别监测尿蛋白、血生化、汞含量、病理等指标，从而确定出1．0 mg／kg为建立模型的合适剂量。另取24只大鼠，随机选取18只为模型组，按上述方法注射1．0 mg／kg氯化汞溶液，另外6只为对照组，并于第15、22、36天将其分别处死，检测肾中汞的蓄积情况。结果染毒后第14天，各模型组与对照组比较均出现了不同程度的尿蛋白升高、血浆白蛋白降低、高脂血症及水肿；病理切片中各剂量组均出现不同程度的肾损伤；血、尿中汞含量有明显的剂量－效应关系。其中1．0 mg／kg剂量组血生化、尿蛋白定量各项指标与生理盐水组比较差异最为明显且水平较稳定，病理检查发现有典型的肾病综合征病变，肾中汞含量检测显示，1．0 mg／kg剂量组出现了汞在肾脏的蓄积。结论 BN大鼠在隔天给药、腹部皮下注射氯化汞1．0 mg／kg、7针停药（2周）的情况下可以建立较理想的汞中毒致大鼠肾病综合征模型。
목적：건립홍중독치대서신병종합정모형，위림상상관치료수단적험증화사선제공은정、과학적험증평태。방법 Brown－Norway rats（ BN대서）웅성24지，수궤분위삼개불동제량적모형조（0．5、1．0급2．0 mg／kg록화홍용액）급일개대조조（생리염수조），격천일차복부피하주사，병재제7、14、21、28、35천분별감측뇨단백、혈생화、홍함량、병리등지표，종이학정출1．0 mg／kg위건립모형적합괄제량。령취24지대서，수궤선취18지위모형조，안상술방법주사1．0 mg／kg록화홍용액，령외6지위대조조，병우제15、22、36천장기분별처사，검측신중홍적축적정황。결과염독후제14천，각모형조여대조조비교균출현료불동정도적뇨단백승고、혈장백단백강저、고지혈증급수종；병리절편중각제량조균출현불동정도적신손상；혈、뇨중홍함량유명현적제량－효응관계。기중1．0 mg／kg제량조혈생화、뇨단백정량각항지표여생리염수조비교차이최위명현차수평교은정，병리검사발현유전형적신병종합정병변，신중홍함량검측현시，1．0 mg／kg제량조출현료홍재신장적축적。결론 BN대서재격천급약、복부피하주사록화홍1．0 mg／kg、7침정약（2주）적정황하가이건립교이상적홍중독치대서신병종합정모형。
Objective The present study aims to establish a model of nephrotic syndrome induced by mercury intoxication, in order to provide stable and scientific platform for clinical relevant treatment.Methods Twenty-four male BN rats were randomly divided into four groups, including three model groups( n=18) and a control group( n=6) .The rats in the three model groups were given respectively mercuric chloride solution by subcutaneous injection of 0.5, 1.0 and 2.0 mg/kg and the control group was given a subcutaneous injection of saline, all the rats were given once every other day. By monitored the urine protein, blood biochemical indicators, mercury pontent, pathological section after injected for 7, 14, 21, 28, 35 days, we identified 1.0 mg/kg was the right dose to establish the model. Another 24 rats were randomly divided into 3 groups, according to the above method the including 18 rats were all injected in 1 mg/kg mercury chloride, the other 6 rats were for the control group.On day 15, 22 and 36 the rats were sacrificed and rat kidneys were sampled to monitor the mercury content in order to observe the accumulation of mercury in the kidney.Results Fourteen days after mercury injection, high proteinuria, low albumin, hyperlipidemia and edema occurred in three model groups, and obvious pathological change of kidney damage was also shown in three model groups.Mercury content in blood and urine showed there was obvious dose -effect relationship.Compared with the control group, the related symptoms were the most prominent and stable with 1mg/kg dose group.There was a typical pathological types with nephrotic syndrome under light microscope with 1 mg/kg dose group and mercury accumulation in kidney tissues after stopped the injection.Conclusion It may be a reliable model of nephrotic syndrome induced by mercury intoxication in BN rats, which are injected subcutaneously with 1 mg/kg mercuric chloride solution once every other day for two weeks.