中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
9期
1621-1625
,共5页
孙晓鹏%李秋杰%刘志林%孙立新%王明山
孫曉鵬%李鞦傑%劉誌林%孫立新%王明山
손효붕%리추걸%류지림%손립신%왕명산
阿司匹林诱生型脂氧素A4%切口疼痛大鼠模型%热缩足潜伏期%机械缩足反应阈值
阿司匹林誘生型脂氧素A4%切口疼痛大鼠模型%熱縮足潛伏期%機械縮足反應閾值
아사필림유생형지양소A4%절구동통대서모형%열축족잠복기%궤계축족반응역치
Aspirin-triggered lipoxin%Incision pain model%Thermal withdrawal latency%Mechanical withdrawal threshold
目的:观察脊髓水平阿司匹林诱生型脂氧素A4(ATL)对切口疼痛大鼠热痛敏、机械痛敏的影响,探讨其是否具有抗伤害性感受作用及其作用机制。方法雄性SD大鼠72只制作动物切口疼痛模型,随机分为4组:生理盐水组(NS组),ATL组,脂氧素受体激动剂组(BML-111组),脂氧素受体拮抗剂组(BOC-2组),每组18只,于手术前(T0),术后2 h(T2)、4 h(T4)、6 h(T6)、8 h(T8)、10 h(T10)通过热板实验、探针刺激实验评估对大鼠热痛敏、机械痛敏的影响。实时PCR检测脊髓炎症因子(TNF-α、IL-6)mRNA的表达,Western blot检测腰段椎体p-ERK的表达。结果组内与T0比较发现,四组切口疼痛模型后2 h,大鼠TWL、MWT均减少(P<0.05), ATL和BML-111组内T4、T6、T8、T10与T2比较,TWL、MWT均增加(P<0.05),NS和Boc-2组内T4、T6、T8、T10与T2比较,TWL、MWT无明显变化。ATL和BML-111两组与NS组组间比较,T4、T6、T8、T10的TWL、MWT均增加(P<0.05)。RT-PCR显示,与 NS 组、Boc-2组比较,ATL组和BML-111组TNF-α、IL-6脊髓mRNA表达明显减少(P<0.05);Western blot结果显示,与NS组、Boc组相比,ATL组、BML-111组p-ERK蛋白表达降低;Boc组与NS组表达无差异。结论切口模型术后后足出现热缩足潜伏期缩短、机械缩足反应阈值减低,鞘内注射阿司匹林诱生型脂氧素 A4可以减轻痛觉过敏,降低脊髓背角神经元中促炎症因子的表达,抑制脊髓p-ERK的表达。
目的:觀察脊髓水平阿司匹林誘生型脂氧素A4(ATL)對切口疼痛大鼠熱痛敏、機械痛敏的影響,探討其是否具有抗傷害性感受作用及其作用機製。方法雄性SD大鼠72隻製作動物切口疼痛模型,隨機分為4組:生理鹽水組(NS組),ATL組,脂氧素受體激動劑組(BML-111組),脂氧素受體拮抗劑組(BOC-2組),每組18隻,于手術前(T0),術後2 h(T2)、4 h(T4)、6 h(T6)、8 h(T8)、10 h(T10)通過熱闆實驗、探針刺激實驗評估對大鼠熱痛敏、機械痛敏的影響。實時PCR檢測脊髓炎癥因子(TNF-α、IL-6)mRNA的錶達,Western blot檢測腰段椎體p-ERK的錶達。結果組內與T0比較髮現,四組切口疼痛模型後2 h,大鼠TWL、MWT均減少(P<0.05), ATL和BML-111組內T4、T6、T8、T10與T2比較,TWL、MWT均增加(P<0.05),NS和Boc-2組內T4、T6、T8、T10與T2比較,TWL、MWT無明顯變化。ATL和BML-111兩組與NS組組間比較,T4、T6、T8、T10的TWL、MWT均增加(P<0.05)。RT-PCR顯示,與 NS 組、Boc-2組比較,ATL組和BML-111組TNF-α、IL-6脊髓mRNA錶達明顯減少(P<0.05);Western blot結果顯示,與NS組、Boc組相比,ATL組、BML-111組p-ERK蛋白錶達降低;Boc組與NS組錶達無差異。結論切口模型術後後足齣現熱縮足潛伏期縮短、機械縮足反應閾值減低,鞘內註射阿司匹林誘生型脂氧素 A4可以減輕痛覺過敏,降低脊髓揹角神經元中促炎癥因子的錶達,抑製脊髓p-ERK的錶達。
목적:관찰척수수평아사필림유생형지양소A4(ATL)대절구동통대서열통민、궤계통민적영향,탐토기시부구유항상해성감수작용급기작용궤제。방법웅성SD대서72지제작동물절구동통모형,수궤분위4조:생리염수조(NS조),ATL조,지양소수체격동제조(BML-111조),지양소수체길항제조(BOC-2조),매조18지,우수술전(T0),술후2 h(T2)、4 h(T4)、6 h(T6)、8 h(T8)、10 h(T10)통과열판실험、탐침자격실험평고대대서열통민、궤계통민적영향。실시PCR검측척수염증인자(TNF-α、IL-6)mRNA적표체,Western blot검측요단추체p-ERK적표체。결과조내여T0비교발현,사조절구동통모형후2 h,대서TWL、MWT균감소(P<0.05), ATL화BML-111조내T4、T6、T8、T10여T2비교,TWL、MWT균증가(P<0.05),NS화Boc-2조내T4、T6、T8、T10여T2비교,TWL、MWT무명현변화。ATL화BML-111량조여NS조조간비교,T4、T6、T8、T10적TWL、MWT균증가(P<0.05)。RT-PCR현시,여 NS 조、Boc-2조비교,ATL조화BML-111조TNF-α、IL-6척수mRNA표체명현감소(P<0.05);Western blot결과현시,여NS조、Boc조상비,ATL조、BML-111조p-ERK단백표체강저;Boc조여NS조표체무차이。결론절구모형술후후족출현열축족잠복기축단、궤계축족반응역치감저,초내주사아사필림유생형지양소 A4가이감경통각과민,강저척수배각신경원중촉염증인자적표체,억제척수p-ERK적표체。
Objective To investigated whether aspirin-triggered lipoxin (ATL) could decrease thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) administered by intrathecal in a rat model of incision pain and the mechanism. Methods Seventy-two male Sprague-Dawley rats were randomly allocated into 4 groups (n=18/group): NS group, ATL group, BML-111 Group and Boc-2 group. All the SD rats were implanted with a catheter intrathecally. The effect of intrathecal administration of all drugs on therma land mechanical threshold were evaluated by hot plate and Frey filaments experiment from preoperation to the postoperative 2nd hour, 6th hour, 8th hour and 10th hour. The expressed of TNF-αand IL-6 mRNA will be assessed by Real-time PCR. Western blot will be carried out for the protein level of phosphorylated extracellular signal-regulated kinase (p-ERK). Results Compared with baseline, the values of TWL and MWT were significantly lower in all groups, but increased at T4, T6, T8, T10 compared with T2 in ATL group and BML-111 group, nor in NS group and Boc-2 group. A comparison of NS group, Boc-2 group and ATL group, BML-111 group indicated that the expression of TNF-αand IL-6 mRNA decreased in the latter two groups. The expressed protein level of ERK showed a marked decrease in ATL and BML-111 group by Western blot, no significant difference existed between NS group and Boc-2 group. Conclusions Aspirin-triggered lipoxin (ATL) elicited antinociceptive effect by decreasing thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) and attenuating the expressed of TNF-α and IL-6 mRNA and protein level of p-ERK administered by intrathecal in a rat model of incision pain.