中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
9期
1617-1620
,共4页
脓毒症%大鼠%肠内免疫营养%免疫营养素%肠集合淋巴结
膿毒癥%大鼠%腸內免疫營養%免疫營養素%腸集閤淋巴結
농독증%대서%장내면역영양%면역영양소%장집합림파결
Sepsis%Rats%Immune enteral nutrition%Immunonutrient%Peyer patches
目的：研究免疫营养素（鸟氨酸-α-酮戊二酸，OKG）强化的肠内营养对腹腔重症感染大鼠肠道免疫功能的影响。方法雄性SD大鼠20只，在行CLP术造成腹腔重症感染后随机分为普通肠内营养组（EN组，n=10）和肠内免疫营养组（EIN组，n=10）。EN组灌喂普通肠内营养液， EIN组灌喂等氮等热量的添加了鸟氨酸-α-酮戊二酸的肠内营养液（EIN）。2组营养剂糖、脂肪、蛋白质供热比为32%∶50%∶18%。术后早期开始灌喂并持续7 d，每日每只大鼠摄入能量标准量为882 kJ·kg-1·d-1。7 d后麻醉处死两组大鼠，取全段小肠，计数总的肠集合淋巴结（PP结）细胞数，流式细胞仪检测PP结的T和B细胞的数目，光镜下观察肠黏膜组织形态学变化，测量肠绒毛高度。结果与普通肠内营养组相比，EIN组总的PP结细胞数、T细胞、B细胞数水平及空肠绒毛高度均高于EN组，差异均有统计学意义（P＜0.05）。结论免疫营养物质OKG强化的肠内营养可以增加脓毒症模型大鼠的肠道PP结及T、B细胞数目，改善其肠道上皮组织结构，增强其免疫功能。
目的：研究免疫營養素（鳥氨痠-α-酮戊二痠，OKG）彊化的腸內營養對腹腔重癥感染大鼠腸道免疫功能的影響。方法雄性SD大鼠20隻，在行CLP術造成腹腔重癥感染後隨機分為普通腸內營養組（EN組，n=10）和腸內免疫營養組（EIN組，n=10）。EN組灌餵普通腸內營養液， EIN組灌餵等氮等熱量的添加瞭鳥氨痠-α-酮戊二痠的腸內營養液（EIN）。2組營養劑糖、脂肪、蛋白質供熱比為32%∶50%∶18%。術後早期開始灌餵併持續7 d，每日每隻大鼠攝入能量標準量為882 kJ·kg-1·d-1。7 d後痳醉處死兩組大鼠，取全段小腸，計數總的腸集閤淋巴結（PP結）細胞數，流式細胞儀檢測PP結的T和B細胞的數目，光鏡下觀察腸黏膜組織形態學變化，測量腸絨毛高度。結果與普通腸內營養組相比，EIN組總的PP結細胞數、T細胞、B細胞數水平及空腸絨毛高度均高于EN組，差異均有統計學意義（P＜0.05）。結論免疫營養物質OKG彊化的腸內營養可以增加膿毒癥模型大鼠的腸道PP結及T、B細胞數目，改善其腸道上皮組織結構，增彊其免疫功能。
목적：연구면역영양소（조안산-α-동무이산，OKG）강화적장내영양대복강중증감염대서장도면역공능적영향。방법웅성SD대서20지，재행CLP술조성복강중증감염후수궤분위보통장내영양조（EN조，n=10）화장내면역영양조（EIN조，n=10）。EN조관위보통장내영양액， EIN조관위등담등열량적첨가료조안산-α-동무이산적장내영양액（EIN）。2조영양제당、지방、단백질공열비위32%∶50%∶18%。술후조기개시관위병지속7 d，매일매지대서섭입능량표준량위882 kJ·kg-1·d-1。7 d후마취처사량조대서，취전단소장，계수총적장집합림파결（PP결）세포수，류식세포의검측PP결적T화B세포적수목，광경하관찰장점막조직형태학변화，측량장융모고도。결과여보통장내영양조상비，EIN조총적PP결세포수、T세포、B세포수수평급공장융모고도균고우EN조，차이균유통계학의의（P＜0.05）。결론면역영양물질OKG강화적장내영양가이증가농독증모형대서적장도PP결급T、B세포수목，개선기장도상피조직결구，증강기면역공능。
Objective To investigate the effects of immune nutrients (OKG) enriched enteral nutrition on intestinal immune function in severe intra bdominal infection rats. Methods After sepsis rats was induced by cecal ligation and puncture (CLP), 20 male SD rats were randomly divided into normal enteral nutrition group (group EN, n=10) and enteral immune nutrition group (group EIN, n=10). EN group was fed with normal enteral nutrition liquid. EIN group was given OKG-enriched enteral nutrition. The two groups rats received isocaloric, isonitrogenous, and isovolumic enteral nutrition. The relative proportions of energy in both groups were 32%carbohydrate, 50%fat and 18%protein. The energy requirement of each rat was calculated according to 882 kJ·kg-1·d-1. The rats were feed in the early postoperative period. Continuous feeding for 7 days, all rats were killed. Collected whole section intestine and counted total cell numbers in peyer’s patches. Used flow cytometry to measure the numbers of T and B cells in peyer’s patches. Changes in the morphology of intestinal mucosa was observed by electron microscopy. Measured the jejunum villus height. Results EIN group compared with the normal enteral nutrition group, the numbers of total cells, T and B cells in Peyer’s patches were significantly higher. The difference was statistical significance (P<0.05). Conclusion OKG-enriched enteral nutrition can increase the numbers of total cells, T and B cells in peyer’s patches in severe intra bdominal infection rats, improve the structure of intestinal epithelial tissue, improve the rats intestine immune function.
