中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2015年
15期
1763-1767
,共5页
陈砚凝%刘尧%王小玲%李芳%刘月平
陳硯凝%劉堯%王小玲%李芳%劉月平
진연응%류요%왕소령%리방%류월평
食管肿瘤%蟾蜍令灵%核糖体蛋白质S6激酶类,70-kDa%原位移植瘤
食管腫瘤%蟾蜍令靈%覈糖體蛋白質S6激酶類,70-kDa%原位移植瘤
식관종류%섬서령령%핵당체단백질S6격매류,70-kDa%원위이식류
Esophageal neoplasms%Bufalin%Ribosomal protein S6 kinases,70 - kDa%Orthotopic transplanted tumor
背景食管癌是世界上最常见的消化道恶性肿瘤之一，近年研究发现，核糖体蛋白 S6激酶（P70S6K）与其发生密切相关。笔者前期的研究表明，蟾蜍令灵（Bufalin）有明显的抗肿瘤作用。目的探讨 Bufalin 对裸鼠食管鳞癌原位移植瘤中 P70S6K 表达的影响。方法 Nu/ Nu 健康裸鼠36只，雌雄各半，采用随机数字表法分为4组，每组9只，即对照组（0.9%氯化钠溶液）、Bufalin 低剂量（0.5 mg/ kg，BL）组、Bufalin 中剂量（1.0 mg/ kg，BM）组、Bufalin 高剂量（1.5 mg/ kg，BH）组。观察经 Bufalin 治疗后裸鼠肿瘤生长情况；反转录 PCR（RT - PCR）法检测裸鼠原位移植瘤中 P70S6K mRNA 的表达，Western blotting 及免疫组化法检测裸鼠原位移植瘤中 P70S6K 和p - P70S6K相对表达量。结果4组治疗前及治疗后第1天裸鼠体质量比较，差异无统计学意义（P >0.05）。4组治疗前肿瘤体积比较，差异无统计学意义（P >0.05）；治疗后第15天肿瘤体积比较，差异有统计学意义（P <0.05）。治疗后第15天，BM组、BH 组肿瘤体积均低于 BL 组和对照组（P <0.05）。3组肿瘤抑制率比较，差异有统计学意义（ P <0.05）。4组P70S6K mRNA 及 P70S6K 相对表达量比较，差异无统计学意义（F =0.634、0.119，P >0.05）。4组p - P70S6K相对表达量比较，差异有统计学意义（F =233.005，P <0.05）。结论 Bufalin 对裸鼠食管鳞癌原位移植瘤具有抑制作用；Bufalin 可以下调 P70S6K 的磷酸化水平，而 P70S6K 则不受影响，提示 Bufalin 可能通过抑制 P70S6K 活化而发挥作用。
揹景食管癌是世界上最常見的消化道噁性腫瘤之一，近年研究髮現，覈糖體蛋白 S6激酶（P70S6K）與其髮生密切相關。筆者前期的研究錶明，蟾蜍令靈（Bufalin）有明顯的抗腫瘤作用。目的探討 Bufalin 對裸鼠食管鱗癌原位移植瘤中 P70S6K 錶達的影響。方法 Nu/ Nu 健康裸鼠36隻，雌雄各半，採用隨機數字錶法分為4組，每組9隻，即對照組（0.9%氯化鈉溶液）、Bufalin 低劑量（0.5 mg/ kg，BL）組、Bufalin 中劑量（1.0 mg/ kg，BM）組、Bufalin 高劑量（1.5 mg/ kg，BH）組。觀察經 Bufalin 治療後裸鼠腫瘤生長情況；反轉錄 PCR（RT - PCR）法檢測裸鼠原位移植瘤中 P70S6K mRNA 的錶達，Western blotting 及免疫組化法檢測裸鼠原位移植瘤中 P70S6K 和p - P70S6K相對錶達量。結果4組治療前及治療後第1天裸鼠體質量比較，差異無統計學意義（P >0.05）。4組治療前腫瘤體積比較，差異無統計學意義（P >0.05）；治療後第15天腫瘤體積比較，差異有統計學意義（P <0.05）。治療後第15天，BM組、BH 組腫瘤體積均低于 BL 組和對照組（P <0.05）。3組腫瘤抑製率比較，差異有統計學意義（ P <0.05）。4組P70S6K mRNA 及 P70S6K 相對錶達量比較，差異無統計學意義（F =0.634、0.119，P >0.05）。4組p - P70S6K相對錶達量比較，差異有統計學意義（F =233.005，P <0.05）。結論 Bufalin 對裸鼠食管鱗癌原位移植瘤具有抑製作用；Bufalin 可以下調 P70S6K 的燐痠化水平，而 P70S6K 則不受影響，提示 Bufalin 可能通過抑製 P70S6K 活化而髮揮作用。
배경식관암시세계상최상견적소화도악성종류지일，근년연구발현，핵당체단백 S6격매（P70S6K）여기발생밀절상관。필자전기적연구표명，섬서령령（Bufalin）유명현적항종류작용。목적탐토 Bufalin 대라서식관린암원위이식류중 P70S6K 표체적영향。방법 Nu/ Nu 건강라서36지，자웅각반，채용수궤수자표법분위4조，매조9지，즉대조조（0.9%록화납용액）、Bufalin 저제량（0.5 mg/ kg，BL）조、Bufalin 중제량（1.0 mg/ kg，BM）조、Bufalin 고제량（1.5 mg/ kg，BH）조。관찰경 Bufalin 치료후라서종류생장정황；반전록 PCR（RT - PCR）법검측라서원위이식류중 P70S6K mRNA 적표체，Western blotting 급면역조화법검측라서원위이식류중 P70S6K 화p - P70S6K상대표체량。결과4조치료전급치료후제1천라서체질량비교，차이무통계학의의（P >0.05）。4조치료전종류체적비교，차이무통계학의의（P >0.05）；치료후제15천종류체적비교，차이유통계학의의（P <0.05）。치료후제15천，BM조、BH 조종류체적균저우 BL 조화대조조（P <0.05）。3조종류억제솔비교，차이유통계학의의（ P <0.05）。4조P70S6K mRNA 급 P70S6K 상대표체량비교，차이무통계학의의（F =0.634、0.119，P >0.05）。4조p - P70S6K상대표체량비교，차이유통계학의의（F =233.005，P <0.05）。결론 Bufalin 대라서식관린암원위이식류구유억제작용；Bufalin 가이하조 P70S6K 적린산화수평，이 P70S6K 칙불수영향，제시 Bufalin 가능통과억제 P70S6K 활화이발휘작용。
Background Esophageal cancer is one of the most common gastrointestinal malignant tumors in the world. According to related studies in recent years,P70S6K is closely related to the occurrence of esophageal cancer. Some previous studies indicate that Bufalin has significant anti - tumor effect. Objectjve To investigate the effects of Bufalin on P70S6K in orthotopic transplanted tumor of esophageal squamous cell carcinoma in nude mice. Methods 36 Nu/ Nu healthy nude mice(18 were male,and 18 were female)were euqally divided into four groups by random number table method:control group (treated with 0. 9% sodium chloride solution),Bufalin low dose group(BL group,treated with 0. 5 mg/ kg Bufalin),Bufalin middle dose group( BM group,treated with 1. 0 mg/ kg Bufalin)and Bufalin high dose group( BH group,treated with 1. 5 mg/ kg Bufalin). The growth of xenografted tumors after Bufalin treatment was observed;the expression of P70S6K mRNA in transplanted tumor in nude mice was examined by RT - PCR method;the expression level of P70S6K and activation level of P70S6K in transplanted tumors of nude mice were detected by western blot and immunohistochemical methods. Results There was no significant difference in body weight of nude mice among four groups before treatment and one day after treatment(P >0. 05). There was no significant difference in tumor volume among four groups before treatment( P > 0. 05 );there was significant difference in tumor volume among four groups 15 days after treatment(P < 0. 05). Tumor volume of mice in BM group and BH group was significantly lower than that of mice in BL group and control group 15 days after treatment,respectively(P <0. 05). There was significant difference in tumor inhibition ratio among three groups( P < 0. 05). There was no significant difference in relative expression levels of P70S6K mRNA and P70S6K among four groups(F = 0. 634,P > 0. 05). There was no significant difference in relative expression level of p - P70S6K among four groups( F = 233. 005,P < 0. 05). Conclusjon Bufalin has an anti - tumor effect on the orthotopic transplanted tumor of esophageal squamous cell carcinoma in nude mice;Bufalin can decrease the level of P70S6K phosphorylation,while P70S6K is not affected,which suggests that Bufalin may inhibit tumor growth through inhibiting the activation of P70S6K.
