中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2015年
14期
1667-1670
,共4页
彭玲%邬超%杨晓红
彭玲%鄔超%楊曉紅
팽령%오초%양효홍
养鸟者肺%肺泡炎,外源性变应性%补体 C5%多态性,单核苷酸%疾病遗传易感性%维吾尔族
養鳥者肺%肺泡炎,外源性變應性%補體 C5%多態性,單覈苷痠%疾病遺傳易感性%維吾爾族
양조자폐%폐포염,외원성변응성%보체 C5%다태성,단핵감산%질병유전역감성%유오이족
Bird fancier's lung%Alveolitis,extrinsic allergic%Complement C5%Polymorphism,single nucleotide%Genetic predisposition to disease%Uygur nationality
目的：探讨补体 C5基因多态性与维吾尔族饲鸽者肺（PBL）易患性的关系。方法选取2012年1月—2013年12月在喀什、和田地区饲养鸽子年限≥1年并在新疆维吾尔自治区人民医院呼吸科病房和门诊确诊的维吾尔族 PBL 患者为病例组（n ＝32），选择饲养鸽子年限≥1年的维吾尔族未患 PBL 者为阴性对照组（n ＝30），选择同期未饲养鸽子且本院体检健康的维吾尔族人群为正常对照组（ n ＝30）。抽取受试者外周血抗凝，提取白细胞 DNA，采用MassARRAY Assay Design 实时 PCR 法进行 C5基因14个单核苷酸多态性（SNP）位点检测，对存在等位基因的 SNP 位点进行组间比较。结果 C5基因 rs17611、rs17612、rs17216529存在基因多态性，其中 rs17611、rs17216529位点存在GG、AA、GA 3种基因型，rs17612位点存在 CC、AA、CA 3种基因型。3组 C5基因 rs17611、rs17216529、rs17612基因型及等位基因频率比较，差异均无统计学意义（P ＞0.05）。结论补体 C5基因多态性可能不影响维吾尔族 PBL 的发病风险。
目的：探討補體 C5基因多態性與維吾爾族飼鴿者肺（PBL）易患性的關繫。方法選取2012年1月—2013年12月在喀什、和田地區飼養鴿子年限≥1年併在新疆維吾爾自治區人民醫院呼吸科病房和門診確診的維吾爾族 PBL 患者為病例組（n ＝32），選擇飼養鴿子年限≥1年的維吾爾族未患 PBL 者為陰性對照組（n ＝30），選擇同期未飼養鴿子且本院體檢健康的維吾爾族人群為正常對照組（ n ＝30）。抽取受試者外週血抗凝，提取白細胞 DNA，採用MassARRAY Assay Design 實時 PCR 法進行 C5基因14箇單覈苷痠多態性（SNP）位點檢測，對存在等位基因的 SNP 位點進行組間比較。結果 C5基因 rs17611、rs17612、rs17216529存在基因多態性，其中 rs17611、rs17216529位點存在GG、AA、GA 3種基因型，rs17612位點存在 CC、AA、CA 3種基因型。3組 C5基因 rs17611、rs17216529、rs17612基因型及等位基因頻率比較，差異均無統計學意義（P ＞0.05）。結論補體 C5基因多態性可能不影響維吾爾族 PBL 的髮病風險。
목적：탐토보체 C5기인다태성여유오이족사합자폐（PBL）역환성적관계。방법선취2012년1월—2013년12월재객십、화전지구사양합자년한≥1년병재신강유오이자치구인민의원호흡과병방화문진학진적유오이족 PBL 환자위병례조（n ＝32），선택사양합자년한≥1년적유오이족미환 PBL 자위음성대조조（n ＝30），선택동기미사양합자차본원체검건강적유오이족인군위정상대조조（ n ＝30）。추취수시자외주혈항응，제취백세포 DNA，채용MassARRAY Assay Design 실시 PCR 법진행 C5기인14개단핵감산다태성（SNP）위점검측，대존재등위기인적 SNP 위점진행조간비교。결과 C5기인 rs17611、rs17612、rs17216529존재기인다태성，기중 rs17611、rs17216529위점존재GG、AA、GA 3충기인형，rs17612위점존재 CC、AA、CA 3충기인형。3조 C5기인 rs17611、rs17216529、rs17612기인형급등위기인빈솔비교，차이균무통계학의의（P ＞0.05）。결론보체 C5기인다태성가능불영향유오이족 PBL 적발병풍험。
Objective To evaluate the association between C5 gene polymorphism and susceptibility to pigeon breeder's lung(PBL)in Uygur people. Methods We enrolled 32 Uygur patients who had bred pigeons for more than one year,came from KashiRegon and Hetian,and were diagnosed with PBL in the outpatient clinic and the Department of Pneumology,the People's Hospital of Xinjiang from January 2012 to December 2013 as the case group. 30 Uygur who had bred pigeons for more than one year and weren't diagnosed with PBL,were enrolled as negative control group. Another 30 healthy Uygur people who hadn't bred pigeons and received physical examination in the hospital in the same period were enrolled as the normal control group. Peripheral blood was sampled from the subjects,and leucocyte DNA was extracted from the samples. We conducted tests on 14 single nucleotide polymorphism( SNP)loci in C5 genes by real - time polymerase chain reaction( PCR)method of MassARRAY Assay Design. The three groups were compared in the SNP loci with allelic genes. Results Gene polymorphism was found in the loci of rs17611,rs17612 and rs17216529. Genotypes of GG,AA and GA were found in rs17611 and rs17216529;genotypes of CC, AA and CA were found in rs17612. No significant differences were found in rs17611, rs17216529 and rs17612 in genotype and allele frequency among three groups ( P > 0. 05 ). Conclusion C5 gene polymorphisms may not have on influence on the susceptibility to PBL in Uygur people.
