中国药物评价
中國藥物評價
중국약물평개
Chinese Journal of Drug Evaluation
2015年
2期
83-86
,共4页
陈惠玲%胡珊梅%陈洁%李玲玲%黄惠琼
陳惠玲%鬍珊梅%陳潔%李玲玲%黃惠瓊
진혜령%호산매%진길%리령령%황혜경
大七厘散%血竭素%大黄%当归%冰片%三七%骨碎补%质量标准
大七釐散%血竭素%大黃%噹歸%冰片%三七%骨碎補%質量標準
대칠전산%혈갈소%대황%당귀%빙편%삼칠%골쇄보%질량표준
Daqili Powder%Dracorhodin%Rhei Radix et Rhizoma%Angelicae sinensis Radix%Borneolum Syntheticum%Notoginseng Radix et Rhizoma%Drynariae Rhizoma%Quality control
目的：研究大七厘散质量标准。方法：建立大黄、当归、冰片、三七及骨碎补的薄层鉴别项。采用HPLC法对血竭中的主要成分血竭素进行含量测定，供试品溶液以12％磷酸甲醇溶液为溶媒的制备，流动相：乙腈-0.05 mol· L－1磷酸二氢钠溶液（50∶50）；检测波长440 nm。结果：血竭素在0.774～77.415μg· mL－1范围内呈良好的线性关系， r ＝0.9999。加样回收率为100.2％，相对标准偏差（RSD）＝1.8％（n＝9）。结论：本法分离效果好，准确可靠，适用于大七厘散的质量控制。
目的：研究大七釐散質量標準。方法：建立大黃、噹歸、冰片、三七及骨碎補的薄層鑒彆項。採用HPLC法對血竭中的主要成分血竭素進行含量測定，供試品溶液以12％燐痠甲醇溶液為溶媒的製備，流動相：乙腈-0.05 mol· L－1燐痠二氫鈉溶液（50∶50）；檢測波長440 nm。結果：血竭素在0.774～77.415μg· mL－1範圍內呈良好的線性關繫， r ＝0.9999。加樣迴收率為100.2％，相對標準偏差（RSD）＝1.8％（n＝9）。結論：本法分離效果好，準確可靠，適用于大七釐散的質量控製。
목적：연구대칠전산질량표준。방법：건립대황、당귀、빙편、삼칠급골쇄보적박층감별항。채용HPLC법대혈갈중적주요성분혈갈소진행함량측정，공시품용액이12％린산갑순용액위용매적제비，류동상：을정-0.05 mol· L－1린산이경납용액（50∶50）；검측파장440 nm。결과：혈갈소재0.774～77.415μg· mL－1범위내정량호적선성관계， r ＝0.9999。가양회수솔위100.2％，상대표준편차（RSD）＝1.8％（n＝9）。결론：본법분리효과호，준학가고，괄용우대칠전산적질량공제。
Objective:To establish quality control for Daqili Powder.Methods: Rhei Radix et Rhizoma, Angelicae sinensis Radix, Borneolum Syntheticum,Notoginseng Radix et Rhizoma, Drynariae Rhizoma in Daqili Powder were identified by TLC,and draorhodin was determined by HPLC.Test solution was made with 12%phosphoric acid in methanol,Use a mixture of acetonitrile and 0.05 mol· L-1 so-dium dihydrogen phosphate solution (50∶50) as the mobile phase.As detector a spectrophotometer set at 440 nm.Results:The calibration curves were linear within the range of 0.774~77.415 μg· mL-1 ( r=0.9999 ).The average recovery was 100.2% with RSD was 1.8%( n=9).Conclusion:The method is sensitive and accurate,and it is suitable for the quality control of Daqili Powder.
