CAJ | 학술논문

目的：评价肝靶向干扰素（ interferon-circumsporozoite protein，IFN-CSP）的免疫毒性作用。方法实验设IFN-CSP低、中、高剂量组（分别为1.87×107 IU／kg，5．6×107 IU／kg，1．68×108 IU／kg），溶剂对照组（生理盐水），阳性对照组（环磷酰胺），小鼠经皮下注射给药2周，迟发型超敏反应检测细胞免疫功能，溶血空斑试验检测体液免疫的功能。结果环磷酰胺阳性对照组与溶剂对照组的小鼠比较，肿胀度、脾指数、胸腺指数明显下降（P＜0.05），低、中、高剂量组与溶剂对照组相比，肿胀度、脾指数、胸腺指数没有显著差异。环磷酰胺阳性对照组的空斑数与溶剂对照组相比明显下降（P＜0．05），而低、中、高剂量组与溶剂对照组相比，空斑数没有显著差异。结论 IFN-CSP对小鼠的细胞免疫功能及体液免疫功能均无明显影响，这为进一步的安全性评价提供了依据。
목적：평개간파향간우소（ interferon-circumsporozoite protein，IFN-CSP）적면역독성작용。방법실험설IFN-CSP저、중、고제량조（분별위1.87×107 IU／kg，5．6×107 IU／kg，1．68×108 IU／kg），용제대조조（생리염수），양성대조조（배린선알），소서경피하주사급약2주，지발형초민반응검측세포면역공능，용혈공반시험검측체액면역적공능。결과배린선알양성대조조여용제대조조적소서비교，종창도、비지수、흉선지수명현하강（P＜0.05），저、중、고제량조여용제대조조상비，종창도、비지수、흉선지수몰유현저차이。배린선알양성대조조적공반수여용제대조조상비명현하강（P＜0．05），이저、중、고제량조여용제대조조상비，공반수몰유현저차이。결론 IFN-CSP대소서적세포면역공능급체액면역공능균무명현영향，저위진일보적안전성평개제공료의거。
Objective To evaluate the immunotoxicity effect of Liver targeting interferon (IFN -CSP) on mice.Methods Mice were randomly divided into five groups:low, middle and high dose of IFN-CSP, solvent control group(saline) and Positive control group (cyclophosphamide).They were injected subcutaneously for 2 weeks.Delayed hypersensitivity test was used to determine the cell immunefunction and plaque forming cell assay was used to determine the humoral immune function.Results There was no significant difference of the the index of immune organ and the ear swelling degree between IFN-CSP groups and control group.There was also no significant difference on hemolytic plaque test between them.Conclusion IFN-CSP has no significant effect on both cellular immunity function and humoral immune function of mice, this results will provides the basis for further safety evaluation.