中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2015年
4期
35-38
,共4页
美洲大蠊%肝癌%多药耐药
美洲大蠊%肝癌%多藥耐藥
미주대렴%간암%다약내약
Periplaneta americana L.%hepatocellular carcinoma%multiple drug resistance
目的：探讨美洲大蠊体外逆转肝癌多药耐药（ multiple drug resistance，MDR）的效果。方法采用阿霉素（ adriamycin， ADM）浓度梯度递增法建立肝癌耐药细胞系HepG2／ADM；台盼蓝染色法测定细胞生长曲线、群体倍增时间改变；MTT法检测肝癌细胞系HepG2和肝癌耐药细胞系HepG2／ADM对4种化疗药物的敏感性；MTT法检测美洲大蠊CⅡ-3及脱脂膏对HepG2／ADM的非毒性剂量；MTT法检测美洲大蠊CⅡ-3、脱脂膏、ADM、CⅡ-3与ADM合用、脱脂膏与ADM合用对HepG2／ADM抑制率的差异。结果耐药细胞株倍增时间较亲本细胞明显延长，HepG2／ADM对多种化疗药物表现出耐药性，其中对ADM耐药指数最高，为15.25倍。当CⅡ-3及脱脂膏浓度分别为28.76，45.08μg／mL时对HepG2及HepG2／ADM 2种细胞株的生长抑制率均＜10％，无明显毒性作用。7.5、15、30μg／mL的CⅡ-3联合ADM （1／2 IC50）对耐药细胞的抑制率分别为（24.52±2.05）％、（34.78±3.08）％、（45.09±3.56）％；12.5、25、50μg／mL的脱脂膏联合ADM （1／2 IC50）对耐药细胞的抑制率分别为（19.28±2.56）％、（35.08±2.13）％、（43.85±3.05）％。结论建立的HepG2／ADM模型具有耐药细胞的基本生物学特性，美洲大蠊提取物能够抑制HepG2／ADM的生长，同时具有良好的逆转其耐药性的效果。
目的：探討美洲大蠊體外逆轉肝癌多藥耐藥（ multiple drug resistance，MDR）的效果。方法採用阿黴素（ adriamycin， ADM）濃度梯度遞增法建立肝癌耐藥細胞繫HepG2／ADM；檯盼藍染色法測定細胞生長麯線、群體倍增時間改變；MTT法檢測肝癌細胞繫HepG2和肝癌耐藥細胞繫HepG2／ADM對4種化療藥物的敏感性；MTT法檢測美洲大蠊CⅡ-3及脫脂膏對HepG2／ADM的非毒性劑量；MTT法檢測美洲大蠊CⅡ-3、脫脂膏、ADM、CⅡ-3與ADM閤用、脫脂膏與ADM閤用對HepG2／ADM抑製率的差異。結果耐藥細胞株倍增時間較親本細胞明顯延長，HepG2／ADM對多種化療藥物錶現齣耐藥性，其中對ADM耐藥指數最高，為15.25倍。噹CⅡ-3及脫脂膏濃度分彆為28.76，45.08μg／mL時對HepG2及HepG2／ADM 2種細胞株的生長抑製率均＜10％，無明顯毒性作用。7.5、15、30μg／mL的CⅡ-3聯閤ADM （1／2 IC50）對耐藥細胞的抑製率分彆為（24.52±2.05）％、（34.78±3.08）％、（45.09±3.56）％；12.5、25、50μg／mL的脫脂膏聯閤ADM （1／2 IC50）對耐藥細胞的抑製率分彆為（19.28±2.56）％、（35.08±2.13）％、（43.85±3.05）％。結論建立的HepG2／ADM模型具有耐藥細胞的基本生物學特性，美洲大蠊提取物能夠抑製HepG2／ADM的生長，同時具有良好的逆轉其耐藥性的效果。
목적：탐토미주대렴체외역전간암다약내약（ multiple drug resistance，MDR）적효과。방법채용아매소（ adriamycin， ADM）농도제도체증법건립간암내약세포계HepG2／ADM；태반람염색법측정세포생장곡선、군체배증시간개변；MTT법검측간암세포계HepG2화간암내약세포계HepG2／ADM대4충화료약물적민감성；MTT법검측미주대렴CⅡ-3급탈지고대HepG2／ADM적비독성제량；MTT법검측미주대렴CⅡ-3、탈지고、ADM、CⅡ-3여ADM합용、탈지고여ADM합용대HepG2／ADM억제솔적차이。결과내약세포주배증시간교친본세포명현연장，HepG2／ADM대다충화료약물표현출내약성，기중대ADM내약지수최고，위15.25배。당CⅡ-3급탈지고농도분별위28.76，45.08μg／mL시대HepG2급HepG2／ADM 2충세포주적생장억제솔균＜10％，무명현독성작용。7.5、15、30μg／mL적CⅡ-3연합ADM （1／2 IC50）대내약세포적억제솔분별위（24.52±2.05）％、（34.78±3.08）％、（45.09±3.56）％；12.5、25、50μg／mL적탈지고연합ADM （1／2 IC50）대내약세포적억제솔분별위（19.28±2.56）％、（35.08±2.13）％、（43.85±3.05）％。결론건립적HepG2／ADM모형구유내약세포적기본생물학특성，미주대렴제취물능구억제HepG2／ADM적생장，동시구유량호적역전기내약성적효과。
Objective To explore the effect of Periplaneta americana reversing multiple drug resistance (MDR) of hepatocellular carcinoma in vitro. Methods Drug resistance of Hepatocellular carcinoma cell line HepG2/ADM was established by Adriamycin ( ADM ) increasing concentration gradient method;determined cell growth curve, doubling time change using Trypan blue staining method;detected sensitivity of 4 kinds of chemotherapeutic drugs of HepG2 and HepG2/ADM by methyl thiazolyl tetrazolium method; detected the non toxic dose of Periplaneta americana CⅡ-3 and the skim cream of HepG2/ADM by MTT method; detected the inhibition rate differences of CⅡ-3, skim cream, ADM, CⅡ-3 and ADM, skim cream and ADM of hepatocellular carcinoma drug resistance cell line HepG2/ADM by MTT method.Results Drug-resistant cell line doubling time was extended obviously compared with parent cells,HepG2/ADM showed resistance to variety of chemotherapeutic drugs, ADM resistant index was especially high,that was 15.25 times.When the concentration of CⅡ-3 and skim cream were 28.76 and 45.08μg/mL respectively, the growth inhibition rate of HepG2 and HepG2/ADM two cell lines were<10%,no obvious toxic effect.The inhibition rate of the drug-resistant cell of 7.5,15,30μg/mL concentration CⅡ-3 joint with ADM concentration (1/2 IC50 ) were (24.52 ±2.05)%,( 34.78 ±3.08)%,( 45.09 ±3.56)% respectively; The inhibition rate of the drug-resistant cell of 12.5,25,50μg/mL concentration skimmed cream joint with ADM concentration (1/2 IC50 ) were (19.28 ±2.56)%,(35.08 ±2.13)%,(43.85 ±3.05)%respectively.Conclusion The cell line HepG2/ADM established has basic multidrug-resistant biological characteristics.Periplaneta americana extract can inhibit the growth of HepG2/ADM,and has good effect to reverse drug resistance at the same time.
