CAJ | 학술논문

目的：探讨美洲大蠊（ Periplaneta americana L.）提取物对人肝癌耐药细胞株HepG2／ADM的作用及其机制。方法将HepG2细胞株应用浓度梯度方法培养出耐阿霉素（ adriamycin，ADM）的HepG2／ADM细胞株。通过免疫细胞化学和RT-PCR方法检测美洲大蠊提取物对P-糖蛋白（ P-glycoprotein，P-gp）、多药耐药相关蛋白（ multidrug resistance-associated protein，MRP）、肺耐药蛋白（ lung resistance protein，LRP）和乳腺耐药蛋白（ breast cancer resistance protein，BCRP）的影响。结果 HepG2／ADM细胞对ADM耐药倍数为10.4，显示较强的耐药性；对DDP、5-FU显示较弱的耐药性；免疫细胞化学法结果表明美洲大蠊提取物能下调HepG2／ADM细胞的P-gp和MRP1蛋白表达量，且具有统计学意义（P＜0.05）；RT-PCR结果表明美洲大蠊提取物仅对HepG2／ADM细胞的P-gp基因表达量下调有统计意义。结论美洲大蠊提取物能够有效逆转HepG2／ADM细胞的耐药性，其作用靶点与P-gp和MRP蛋白及P-gp基因有关。
목적：탐토미주대렴（ Periplaneta americana L.）제취물대인간암내약세포주HepG2／ADM적작용급기궤제。방법장HepG2세포주응용농도제도방법배양출내아매소（ adriamycin，ADM）적HepG2／ADM세포주。통과면역세포화학화RT-PCR방법검측미주대렴제취물대P-당단백（ P-glycoprotein，P-gp）、다약내약상관단백（ multidrug resistance-associated protein，MRP）、폐내약단백（ lung resistance protein，LRP）화유선내약단백（ breast cancer resistance protein，BCRP）적영향。결과 HepG2／ADM세포대ADM내약배수위10.4，현시교강적내약성；대DDP、5-FU현시교약적내약성；면역세포화학법결과표명미주대렴제취물능하조HepG2／ADM세포적P-gp화MRP1단백표체량，차구유통계학의의（P＜0.05）；RT-PCR결과표명미주대렴제취물부대HepG2／ADM세포적P-gp기인표체량하조유통계의의。결론미주대렴제취물능구유효역전HepG2／ADM세포적내약성，기작용파점여P-gp화MRP단백급P-gp기인유관。
Objective To investigate the effect of extracts from Periplaneta americana L.on reversing MDR in HepG2/ADM.Methods HepG2/ADM was induced by continuous stepwise exposer to ADM.To investigate the effect of extractive from Periplaneta americana L.on drug resistance-associated protein( P-gp、MRP、LRP和BCRP) by immunocytochemical method and RT-PCR method.Results HepG2/ADM cells to ADM shows a strong multiple drug resistance that is 10.4 times,For DDP,5-FU showed weak resistance.The extractive from Periplaneta americana L.can cut the expression of P-gp and MRP1 protein in HepG2/ADM from immunocytochemical method,and it has statistically significant.The extractive from Periplaneta americana L.can cut the expression of P-gp gene,and it has statistically significant.Conclusion The extract from Periplaneta americana L.can effectively reverse MDR in HepG2/ADM,which targets for P-gp and MRP1 protein and P-gp gene of HepG2/ADM cells.