CAJ | 학술논문

目的：研究紫草素对人宫颈癌Hela细胞增殖的影响及其作用机制。方法用MTT法检测紫草素对人宫颈癌Hela细胞的增殖抑制作用；通过测定内源性活性氧、线粒体膜电位，探讨紫草素对Hela细胞氧化损伤的作用机制。结果紫草素抑制Hela细胞增殖且呈剂量依赖性。紫草素可剂量依赖性地升高Hela细胞内源性活性氧水平，降低线粒体膜电位，抗氧化剂NAC能显著性的降低紫草素的抗肿瘤活性。结论线粒体膜电位的改变和内源性活性氧的生成是紫草素抑制人宫颈癌Hela细胞增殖的作用机制之一。
목적：연구자초소대인궁경암Hela세포증식적영향급기작용궤제。방법용MTT법검측자초소대인궁경암Hela세포적증식억제작용；통과측정내원성활성양、선립체막전위，탐토자초소대Hela세포양화손상적작용궤제。결과자초소억제Hela세포증식차정제량의뢰성。자초소가제량의뢰성지승고Hela세포내원성활성양수평，강저선립체막전위，항양화제NAC능현저성적강저자초소적항종류활성。결론선립체막전위적개변화내원성활성양적생성시자초소억제인궁경암Hela세포증식적작용궤제지일。
Objective To study effect of Shikonin on human cervical cancer Hela cell growth suppression in vitro and its mechanism. Methods MTT assay was used to examine the growth inhibition of Shikonin in Hela cells.And then, the measurement of both ROS Levels and Mitochondrial Membrane Potential (ΔΨm ) were performed to clarify the mechanism of antitumor in Hela cells by Shikonin.Results Shikonin significantly inhibited the growth of Hela cells in a concentration-dependent manner.Shikonin increased generation of en-dogenous reactive oxygen species ( ROS) and decreased mitochondrial membrane potential.Furthermore, anti-oxidants N-acetylcysteine ( NAC) could significantly reduce the antitumor activity of SK in Hela cells.Conclusion These results suggest that mitochondrial aerobic respiration shift and endogenous ROS augmentation contribute to the action of Shikonin against Hela cells.