中国生化药物杂志
中國生化藥物雜誌
중국생화약물잡지
CHINESE JOURNAL OF BIOCHEMICAL PHARMACEUTICS
2015年
4期
13-15,18
,共4页
辛叶%田倩%曹海军%李长清
辛葉%田倩%曹海軍%李長清
신협%전천%조해군%리장청
凝血酶原复合物%抗凝血酶%肝素%促凝能力%抗凝能力
凝血酶原複閤物%抗凝血酶%肝素%促凝能力%抗凝能力
응혈매원복합물%항응혈매%간소%촉응능력%항응능력
prothrombin complex concentrates%antithrombin%heparin%procoagulation activities%anticoagulation activities
目的：探讨人凝血酶原复合物（ prothrombin complex concentrates，PCC）中不同水平抗凝血酶（ antithrombin，AT）和肝素的对其促凝和抗凝能力的影响。方法首先，制备仅含微量AT和肝素的PCC。其次，设定AT和肝素2个可变因素，每因素分别取3水平：0、0.4、0.8 IU／mL；0、2.5、5.0 IU／mL，设计正交试验。最后，分析AT和肝素对PCC促凝和抗凝能力的影响。结果 AT对PCC凝血酶抑制能力影响的极差高于肝素。随着AT剂量的增加，达峰时间逐渐延长。0.4 IU／mL和0.8 IU／mL AT的延迟时间相同。0.4 IU／mL AT的峰值最高。 AT剂量越大对凝血酶抑制能力影响越大，而0.4 IU／mL和0.8 IU／mL AT对抑制能力的影响差别较小。肝素对PCC凝血酶生成的影响的极差高于AT。随着肝素剂量的增加，延迟时间和达峰时间都随之延长，而峰值则随之下降，0 IU／mL 和2.5 IU／mL肝素对凝血酶生成的影响差别较小。肝素水平越高则对凝血酶抑制能力影响越大，而2.5 IU／mL和5.0 IU／mL肝素对凝血酶抑制能力影响差别较小。结论 AT对PCC抗凝能力的影响大于肝素，而肝素对PCC促凝能力的影响大于AT。添加中等水平的AT和肝素的PCC在促凝和抗凝功能之间取得了较好的平衡。
目的：探討人凝血酶原複閤物（ prothrombin complex concentrates，PCC）中不同水平抗凝血酶（ antithrombin，AT）和肝素的對其促凝和抗凝能力的影響。方法首先，製備僅含微量AT和肝素的PCC。其次，設定AT和肝素2箇可變因素，每因素分彆取3水平：0、0.4、0.8 IU／mL；0、2.5、5.0 IU／mL，設計正交試驗。最後，分析AT和肝素對PCC促凝和抗凝能力的影響。結果 AT對PCC凝血酶抑製能力影響的極差高于肝素。隨著AT劑量的增加，達峰時間逐漸延長。0.4 IU／mL和0.8 IU／mL AT的延遲時間相同。0.4 IU／mL AT的峰值最高。 AT劑量越大對凝血酶抑製能力影響越大，而0.4 IU／mL和0.8 IU／mL AT對抑製能力的影響差彆較小。肝素對PCC凝血酶生成的影響的極差高于AT。隨著肝素劑量的增加，延遲時間和達峰時間都隨之延長，而峰值則隨之下降，0 IU／mL 和2.5 IU／mL肝素對凝血酶生成的影響差彆較小。肝素水平越高則對凝血酶抑製能力影響越大，而2.5 IU／mL和5.0 IU／mL肝素對凝血酶抑製能力影響差彆較小。結論 AT對PCC抗凝能力的影響大于肝素，而肝素對PCC促凝能力的影響大于AT。添加中等水平的AT和肝素的PCC在促凝和抗凝功能之間取得瞭較好的平衡。
목적：탐토인응혈매원복합물（ prothrombin complex concentrates，PCC）중불동수평항응혈매（ antithrombin，AT）화간소적대기촉응화항응능력적영향。방법수선，제비부함미량AT화간소적PCC。기차，설정AT화간소2개가변인소，매인소분별취3수평：0、0.4、0.8 IU／mL；0、2.5、5.0 IU／mL，설계정교시험。최후，분석AT화간소대PCC촉응화항응능력적영향。결과 AT대PCC응혈매억제능력영향적겁차고우간소。수착AT제량적증가，체봉시간축점연장。0.4 IU／mL화0.8 IU／mL AT적연지시간상동。0.4 IU／mL AT적봉치최고。 AT제량월대대응혈매억제능력영향월대，이0.4 IU／mL화0.8 IU／mL AT대억제능력적영향차별교소。간소대PCC응혈매생성적영향적겁차고우AT。수착간소제량적증가，연지시간화체봉시간도수지연장，이봉치칙수지하강，0 IU／mL 화2.5 IU／mL간소대응혈매생성적영향차별교소。간소수평월고칙대응혈매억제능력영향월대，이2.5 IU／mL화5.0 IU／mL간소대응혈매억제능력영향차별교소。결론 AT대PCC항응능력적영향대우간소，이간소대PCC촉응능력적영향대우AT。첨가중등수평적AT화간소적PCC재촉응화항응공능지간취득료교호적평형。
Objective To study the influence of prothrombin complex concentrates (PCC) containing different levels of antithrombin (AT) and heparin on its procoagulation and anticoagulation activities.Methods PCC containing traces of heparin and AT were prepared.The design of orthogonal experiment: set AT and heparin two variable factors, each factor respectively from three levels: 0, 0.4, 0.8 IU/mL; 0, 2.5, 5.0 IU/mL.Their influence on the procoagulation and anticoagulation activities of PCC were analysed.Results The R value of the influence of AT on thrombin inhibitory capacities was higher than that of heparin.With the increase of AT level, time to peak gradually extended.Lag time of 0.4 IU/mL and 0.8 IU/mL AT was the same.The peak height of 0.4 IU/mL AT was highest.The higher the level of AT, the greater the impact on thrombin inhibitory capacities.0.4 IU/mL and 0.8 IU/mL AT had similar impact on thrombin inhibitory capacities.The R values of the influence of heparin on thrombin generation were higher than that of AT.With the increase of heparin level, lag time and time to peak were longer while the peak height declined sharply.0 IU/mL and 2.5 IU/mL heparin had similar influence on thrombin generation.The higher the level of heparin, the greater influence on thrombin inhibitory capacities.2.5 IU/mL and 5.0 IU/mL heparin had similar influence on thrombin inhibitory capacities.Conclusion AT has greater influence on the anticoagulation activities of PCC than that of heparin, while heparin has greater influence on the procoagulation activities of PCC than that of AT.PCC containing medium levels of AT and heparin is relatively balanced between procoagulation and anticoagulation.
