CAJ | 학술논문

目的：研究加味四君子汤对碱烧伤食管组织中p53和Bcl-2表达水平的影响。方法采用不同浓度的氢氧化钠注入家兔食管，建立烧伤模型，对食管进行解剖学观察。采用高、低剂量的加味四君子汤对家兔灌胃，1次／天，20d后用氢氧化钠烧伤食管，显微镜下观察各组食管上皮细胞组织形态。采用免疫印迹法和定量反转录聚合酶链式反应检测食管细胞中p53和Bcl-2的蛋白和mRNA表达水平。结果食管上皮细胞的腐蚀程度与氢氧化钠浓度呈正相关。 Western blot和qRT-PCR结果表明，低、高剂量加味四君子汤可抑制化学性烧伤所引起p53的蛋白和mRNA表达水平的增加；而明显逆转化学性烧伤所引起Bcl-2的蛋白和mRNA表达水平的降低。结论加味四君子汤呈浓度依赖性缓解碱对食管的烧伤。提示加味四君子汤具有保护食管粘膜，预防食管化学性烧伤后癌变的功效。
목적：연구가미사군자탕대감소상식관조직중p53화Bcl-2표체수평적영향。방법채용불동농도적경양화납주입가토식관，건립소상모형，대식관진행해부학관찰。채용고、저제량적가미사군자탕대가토관위，1차／천，20d후용경양화납소상식관，현미경하관찰각조식관상피세포조직형태。채용면역인적법화정량반전록취합매련식반응검측식관세포중p53화Bcl-2적단백화mRNA표체수평。결과식관상피세포적부식정도여경양화납농도정정상관。 Western blot화qRT-PCR결과표명，저、고제량가미사군자탕가억제화학성소상소인기p53적단백화mRNA표체수평적증가；이명현역전화학성소상소인기Bcl-2적단백화mRNA표체수평적강저。결론가미사군자탕정농도의뢰성완해감대식관적소상。제시가미사군자탕구유보호식관점막，예방식관화학성소상후암변적공효。
Objective To establish burn-induced esophageal lesion model by adding NaOH with different concentrations in rabbits, and investigate the effect of modified sijunzi decoction on the p53 and Bcl-2.Methods After injection with different concentrations of NaOH, esophagus was dissected and observed anatomically.Rabbits were given a gavage of modified Sijunzi decoction daily for 20 days, and then were injected with NaOH.Esophageal epithelium isolated from each group was observed by hematoxylin-eosin staining.p53 and Bcl-2 protein and mRNA expression was measured with western blot and qRT-PCR, respectively.Results The degree of corrosion of esophageal epithelium was positively correlated with the concentration of NaOH.p53 protein and mRNA levels were increased after NaOH challenge; this increase was inhibited by treatment with modified Sijunzi decoction.Additionally, NaOH decreased Bcl-2 protein and mRNA, which was attenuated by modified Sijunzi decoction.Conclusion Modified sijunzi decoction can relieve the esophageal alkali burning in a dose-dependent manner, suggesting that modified Sijunzi decoction may be a useful strategy to treat chemical injuries in esophageal tissue.