CAJ | 학술논문

目的：探讨建立兔高甲状腺素心房颤动（房颤）易患模型，并检测左心房电生理指标变化。方法日本大耳兔49只随机分为3组，对照组（10只，每日注射生理盐水）、撤药组（20只，每日注射甲状腺素50μg／kg，2月后撤药改注射等剂量生理盐水）、持续给药组（19只，每日注射甲状腺素50μg／kg），实验时间均为4月。收集各组左心房有效不应期（AERP）、激动传导速度（CV）、激动波长（WL）和房颤诱发率等数据。结果干预2月后撤药组、持续给药组较对照组AERP200、AERP150均缩短（P＜0.05），4月后持续给药组较撤药组、对照组AERP200、AERP150明显缩短（P＜0.01）；干预2月后撤药组、持续给药组较对照组CV减慢（P＜0.05），4月后持续给药组较撤药组、对照组CV明显减慢（P＜0.01）；干预2月后撤药组、持续给药组较对照组WL缩短（P＜0.05），4月后持续给药组较撤药组、对照组CV明显缩短（ P＜0.01）；对照组没有诱发出房颤，4月后持续给药组房颤诱发率显著增高，撤药组下降。结论制作高甲状腺素兔房颤易患模型切实可行，并伴有左心房电生理指标改变，为进一步研究甲亢合并房颤发病机制提供动物实验基础。
목적：탐토건립토고갑상선소심방전동（방전）역환모형，병검측좌심방전생리지표변화。방법일본대이토49지수궤분위3조，대조조（10지，매일주사생리염수）、철약조（20지，매일주사갑상선소50μg／kg，2월후철약개주사등제량생리염수）、지속급약조（19지，매일주사갑상선소50μg／kg），실험시간균위4월。수집각조좌심방유효불응기（AERP）、격동전도속도（CV）、격동파장（WL）화방전유발솔등수거。결과간예2월후철약조、지속급약조교대조조AERP200、AERP150균축단（P＜0.05），4월후지속급약조교철약조、대조조AERP200、AERP150명현축단（P＜0.01）；간예2월후철약조、지속급약조교대조조CV감만（P＜0.05），4월후지속급약조교철약조、대조조CV명현감만（P＜0.01）；간예2월후철약조、지속급약조교대조조WL축단（P＜0.05），4월후지속급약조교철약조、대조조CV명현축단（ P＜0.01）；대조조몰유유발출방전，4월후지속급약조방전유발솔현저증고，철약조하강。결론제작고갑상선소토방전역환모형절실가행，병반유좌심방전생리지표개변，위진일보연구갑항합병방전발병궤제제공동물실험기출。
Objective To investigate about establishment the animal model of atrial fibrillation(AF) by high thyroxine and electrophysiological study of left atrium.Methods 49 rabbits were randomly divided into three groups, control group (10, injection of saline), withdrawal group (20, injection of levo-thyroxine 50μg/kg, change to inject isodose saline after two months), continuous dosing group (19, injection of levo-thyroxine 50μg/kg everyday).the data of left atrium effective refractory period(AERP), conduction velocity(CV), wavelength(WL) and AF induced ratio were collected after four months.Results The withdrawal group and continuous dosing group AERP200, AERP150 were more shorter than the control group after two months(P<0.05), The continuous dosing group AERP200, AERP150 was shorter significantly than withdrawal group and control group after four months ( P<0.01 ).The withdrawal group and continuous dosing group CV were slower than control group after two months ( P<0.05 ).The continuous dosing group CV was slower significantly than withdrawal group and control group after four months ( P<0.01 ).The withdrawal group and continuous dosing group WL were shorter than control group after two months(P<0.05), The continuous dosing group WL was shorter significantly than withdrawal group and control group after four months (P<0.01).The AF induced ratio in the continuous dosing group increased significantly(P<0.01). After four months, but the withdrawal group decreased, the control group did not induce AF.Conclusion It's feasible to establish the rabbit model of AF by high thyroxine, with left atrium electrophysiological changes, which provides animal model for further to study the pathogenesis of AF cause of hyperthyroidism.