CAJ | 학술논문

针对微藻采收成本高的难题,以一株微拟球藻( Nannochloropsis sp.)为研究材料,采用响应面法优化该藻株的高pH沉降采收技术,并初步评估其培养基的循环利用。结果显示,藻株培养液的细胞密度、沉降pH及处理时间3种因素对其生物量采收率( BRR)具有显著影响。预测最优采收参数为：藻细胞光密度OD750=2.08；沉降pH=12.20；处理时间12 h,预测BRR为98.97%,验证实验实测BRR为98.31%。采收藻细胞后的上清液经过滤、pH调整及营养盐补充后,探究高pH采收后的培养基上清液的循环利用。与新鲜培养基相比,高pH采收回收培养基中的藻细胞生长和油脂含量有小幅度的降低,但总脂分级特性及脂肪酸组成无显著差异。该藻株的高pH采收及其培养基的回收利用方法具有可行性。
침대미조채수성본고적난제,이일주미의구조( Nannochloropsis sp.)위연구재료,채용향응면법우화해조주적고pH침강채수기술,병초보평고기배양기적순배이용。결과현시,조주배양액적세포밀도、침강pH급처리시간3충인소대기생물량채수솔( BRR)구유현저영향。예측최우채수삼수위：조세포광밀도OD750=2.08；침강pH=12.20；처리시간12 h,예측BRR위98.97%,험증실험실측BRR위98.31%。채수조세포후적상청액경과려、pH조정급영양염보충후,탐구고pH채수후적배양기상청액적순배이용。여신선배양기상비,고pH채수회수배양기중적조세포생장화유지함량유소폭도적강저,단총지분급특성급지방산조성무현저차이。해조주적고pH채수급기배양기적회수이용방법구유가행성。
High-cost harvesting technology is a limitation for industrial cultivation of microglgae. This report deals with the optimization of the high-pH-induced flocculation of a strain of Nannochloropsis sp. using the response surface methodology ( RSM ) , with which a three-variable, three-level experiment Box-Behnken design ( BBD) was developed to examine the effect of initial microalgal cell density ( OD750 ) , pH value and treating time on harvesting efficiency ( biomass recovery rate, BRR) of the strain. The results showed that all the three factors significantly influenced the BRR, and the optimum flocculation parameters were as follows:OD750:2. 08, flocculating pH:12. 20, and treatment time:12 h. Under the optimal condition, the value of BRR attained 98. 31%, agreed well with the predicted value (98. 97%). The feasibility of reusing the culture medium after high PH harvesting was further studied after the harvested supernatant treated with pH neutralization and nutrients supplementation. The results indicated that compared with the fresh medium, the medium made with high-pH harvesting supernatant resulted in a slight decrease in growth yield and lipid content, and no obvious changes in lipid distribution and fatty acid profiles, illustrating that reuse of culture medium after high pH-harvesting was acceptable for Nannochloropsis sp. production.