中华妇产科杂志
中華婦產科雜誌
중화부산과잡지
CHINESE JOUNAL OF OBSTETRICS AND GYNECOLOGY
2015年
4期
283-290
,共8页
庄白妹%罗欣%饶海英%李庆姝%刘西茹%漆洪波
莊白妹%囉訢%饒海英%李慶姝%劉西茹%漆洪波
장백매%라흔%요해영%리경주%류서여%칠홍파
先兆子痫%胎盘%核基质附着区结合蛋白质类%Wnt蛋白质类%β连环素%基质金属蛋白酶2%基质金属蛋白酶9
先兆子癇%胎盤%覈基質附著區結閤蛋白質類%Wnt蛋白質類%β連環素%基質金屬蛋白酶2%基質金屬蛋白酶9
선조자간%태반%핵기질부착구결합단백질류%Wnt단백질류%β련배소%기질금속단백매2%기질금속단백매9
Pre-eclampsia%Placenta%Matrix attachment region binding proteins%Wnt proteins%Beta catenin%Matrix metalloproteinase 2%Matrix metalloproteinase 9
目的：探讨富含AT序列的特异性结合蛋白1（SATB1）及蛋白（wnt）/β连环蛋白（β-catenin）信号通路相关分子在调控滋养细胞侵袭以及在子痫前期发病中的作用。方法收集2013年3月—2014年3月在重庆医科大学附属第一医院行人工流产术的20例孕8~10周孕妇的绒毛组织（早孕组）；同期在妇产科住院行水囊引产的18例中孕期（孕18~20周）孕妇的胎盘组织（中孕组）；同期行择期剖宫产术的20例健康足月妊娠孕妇的胎盘组织（正常足月组）；同期住院行选择性剖宫产术分娩的20例子痫前期孕妇（孕33~38周）的胎盘组织（子痫前期组）。采用免疫组化SP法检测各组绒毛或胎盘组织中SATB1及β-catenin的蛋白表达定位；采用蛋白印迹法检测各组绒毛或胎盘组织中SATB1及β-catenin的蛋白表达水平；采用细胞免疫荧光法检测SATB1及β-catenin在滋养细胞株HTR8/SVneo细胞中的定位表达，并对细胞中SATB1及β-catenin蛋白表达水平进行检测；采用免疫共沉淀法检测子痫前期组胎盘组织和缺氧/复氧组HTR8/SVneo细胞中的SATB1与β-catenin的相互关系；采用明胶酶谱法检测子痫前期组和缺氧/复氧组细胞中基质金属蛋白酶（MMP）2、9的活性水平。结果（1）正常足月组胎盘组织合体滋养细胞结节和纤维素样坏死很少见，毛细血管数目丰富；子痫前期组胎盘组织可见合体滋养细胞胞核空泡化明显，有较多纤维素样坏死，绒毛内微血管数目减少。（2）各组绒毛或胎盘组织中均可见SATB1棕黄色染色颗粒，子痫前期组胎盘组织中SATB1阳性染色强度较正常足月组明显减弱。（3）各组绒毛或胎盘组织中均可见β-catenin蛋白的表达，子痫前期组胎盘组织中β-catenin阳性染色强度较正常足月组减弱。（4）早孕组、中孕组、正常足月组及子痫前期组中SATB1蛋白表达水平分别为0.300±0.009、0.271±0.015、0.238±0.018及0.153±0.007；β-catenin蛋白表达水平分别为0.743±0.041、0.648±0.021、0.549±0.069及0.269±0.047。子痫前期组SATB1及β-catenin蛋白表达水平均较早孕组、中孕组、正常足月组明显下降，分别比较，差异均有统计学意义（P<0.05）。（5）常氧组及缺氧/复氧组SATB1蛋白定位于细胞滋养细胞的胞核中，缺氧/复氧组细胞的荧光强度较常氧组细胞明显减弱；常氧组及缺氧/复氧组β-catenin蛋白定位于细胞滋养细胞的胞核及胞质中，缺氧/复氧组细胞的荧光强度较常氧组细胞明显减弱。（6）常氧组细胞SATB1及β-catenin蛋白表达水平分别为0.213±0.005和0.797±0.081，而缺氧/复氧组分别为0.083±0.021和0.543±0.131。两组分别比较，差异均有统计学意义（P<0.05）。（7）子痫前期组胎盘组织中和缺氧/复氧组细胞中经SATB1抗体免疫共沉淀后的蛋白复合物中，均可检测到β-catenin蛋白的表达；同样，经β-catenin抗体免疫共沉淀后的蛋白复合物中，也均可检测到SATB1蛋白的表达。（8）子痫前期组胎盘组织中MMP-2、9活性水平分别为2.251±0.310、1.447±0.102，正常足月组分别为7.098±0.451、5.502±0.197，两组分别比较，差异有统计学意义（P<0.05）。缺氧/复氧组细胞中MMP-2、9活性水平分别为0.471±0.104、0.297±0.103，常氧组分别为0.842±0.209、0.595±0.100，缺氧/复氧组细胞明显低于常氧组，分别比较，差异均有统计学意义（P<0.05）。结论子痫前期胎盘组织中SATB1表达水平下降，可能是通过调控wnt/β-catenin信号通路而影响MMP-2、9的活性水平改变，使滋养细胞的侵袭力减弱，最终导致子痫前期的发生。
目的：探討富含AT序列的特異性結閤蛋白1（SATB1）及蛋白（wnt）/β連環蛋白（β-catenin）信號通路相關分子在調控滋養細胞侵襲以及在子癇前期髮病中的作用。方法收集2013年3月—2014年3月在重慶醫科大學附屬第一醫院行人工流產術的20例孕8~10週孕婦的絨毛組織（早孕組）；同期在婦產科住院行水囊引產的18例中孕期（孕18~20週）孕婦的胎盤組織（中孕組）；同期行擇期剖宮產術的20例健康足月妊娠孕婦的胎盤組織（正常足月組）；同期住院行選擇性剖宮產術分娩的20例子癇前期孕婦（孕33~38週）的胎盤組織（子癇前期組）。採用免疫組化SP法檢測各組絨毛或胎盤組織中SATB1及β-catenin的蛋白錶達定位；採用蛋白印跡法檢測各組絨毛或胎盤組織中SATB1及β-catenin的蛋白錶達水平；採用細胞免疫熒光法檢測SATB1及β-catenin在滋養細胞株HTR8/SVneo細胞中的定位錶達，併對細胞中SATB1及β-catenin蛋白錶達水平進行檢測；採用免疫共沉澱法檢測子癇前期組胎盤組織和缺氧/複氧組HTR8/SVneo細胞中的SATB1與β-catenin的相互關繫；採用明膠酶譜法檢測子癇前期組和缺氧/複氧組細胞中基質金屬蛋白酶（MMP）2、9的活性水平。結果（1）正常足月組胎盤組織閤體滋養細胞結節和纖維素樣壞死很少見，毛細血管數目豐富；子癇前期組胎盤組織可見閤體滋養細胞胞覈空泡化明顯，有較多纖維素樣壞死，絨毛內微血管數目減少。（2）各組絨毛或胎盤組織中均可見SATB1棕黃色染色顆粒，子癇前期組胎盤組織中SATB1暘性染色彊度較正常足月組明顯減弱。（3）各組絨毛或胎盤組織中均可見β-catenin蛋白的錶達，子癇前期組胎盤組織中β-catenin暘性染色彊度較正常足月組減弱。（4）早孕組、中孕組、正常足月組及子癇前期組中SATB1蛋白錶達水平分彆為0.300±0.009、0.271±0.015、0.238±0.018及0.153±0.007；β-catenin蛋白錶達水平分彆為0.743±0.041、0.648±0.021、0.549±0.069及0.269±0.047。子癇前期組SATB1及β-catenin蛋白錶達水平均較早孕組、中孕組、正常足月組明顯下降，分彆比較，差異均有統計學意義（P<0.05）。（5）常氧組及缺氧/複氧組SATB1蛋白定位于細胞滋養細胞的胞覈中，缺氧/複氧組細胞的熒光彊度較常氧組細胞明顯減弱；常氧組及缺氧/複氧組β-catenin蛋白定位于細胞滋養細胞的胞覈及胞質中，缺氧/複氧組細胞的熒光彊度較常氧組細胞明顯減弱。（6）常氧組細胞SATB1及β-catenin蛋白錶達水平分彆為0.213±0.005和0.797±0.081，而缺氧/複氧組分彆為0.083±0.021和0.543±0.131。兩組分彆比較，差異均有統計學意義（P<0.05）。（7）子癇前期組胎盤組織中和缺氧/複氧組細胞中經SATB1抗體免疫共沉澱後的蛋白複閤物中，均可檢測到β-catenin蛋白的錶達；同樣，經β-catenin抗體免疫共沉澱後的蛋白複閤物中，也均可檢測到SATB1蛋白的錶達。（8）子癇前期組胎盤組織中MMP-2、9活性水平分彆為2.251±0.310、1.447±0.102，正常足月組分彆為7.098±0.451、5.502±0.197，兩組分彆比較，差異有統計學意義（P<0.05）。缺氧/複氧組細胞中MMP-2、9活性水平分彆為0.471±0.104、0.297±0.103，常氧組分彆為0.842±0.209、0.595±0.100，缺氧/複氧組細胞明顯低于常氧組，分彆比較，差異均有統計學意義（P<0.05）。結論子癇前期胎盤組織中SATB1錶達水平下降，可能是通過調控wnt/β-catenin信號通路而影響MMP-2、9的活性水平改變，使滋養細胞的侵襲力減弱，最終導緻子癇前期的髮生。
목적：탐토부함AT서렬적특이성결합단백1（SATB1）급단백（wnt）/β련배단백（β-catenin）신호통로상관분자재조공자양세포침습이급재자간전기발병중적작용。방법수집2013년3월—2014년3월재중경의과대학부속제일의원행인공유산술적20례잉8~10주잉부적융모조직（조잉조）；동기재부산과주원행수낭인산적18례중잉기（잉18~20주）잉부적태반조직（중잉조）；동기행택기부궁산술적20례건강족월임신잉부적태반조직（정상족월조）；동기주원행선택성부궁산술분면적20례자간전기잉부（잉33~38주）적태반조직（자간전기조）。채용면역조화SP법검측각조융모혹태반조직중SATB1급β-catenin적단백표체정위；채용단백인적법검측각조융모혹태반조직중SATB1급β-catenin적단백표체수평；채용세포면역형광법검측SATB1급β-catenin재자양세포주HTR8/SVneo세포중적정위표체，병대세포중SATB1급β-catenin단백표체수평진행검측；채용면역공침정법검측자간전기조태반조직화결양/복양조HTR8/SVneo세포중적SATB1여β-catenin적상호관계；채용명효매보법검측자간전기조화결양/복양조세포중기질금속단백매（MMP）2、9적활성수평。결과（1）정상족월조태반조직합체자양세포결절화섬유소양배사흔소견，모세혈관수목봉부；자간전기조태반조직가견합체자양세포포핵공포화명현，유교다섬유소양배사，융모내미혈관수목감소。（2）각조융모혹태반조직중균가견SATB1종황색염색과립，자간전기조태반조직중SATB1양성염색강도교정상족월조명현감약。（3）각조융모혹태반조직중균가견β-catenin단백적표체，자간전기조태반조직중β-catenin양성염색강도교정상족월조감약。（4）조잉조、중잉조、정상족월조급자간전기조중SATB1단백표체수평분별위0.300±0.009、0.271±0.015、0.238±0.018급0.153±0.007；β-catenin단백표체수평분별위0.743±0.041、0.648±0.021、0.549±0.069급0.269±0.047。자간전기조SATB1급β-catenin단백표체수평균교조잉조、중잉조、정상족월조명현하강，분별비교，차이균유통계학의의（P<0.05）。（5）상양조급결양/복양조SATB1단백정위우세포자양세포적포핵중，결양/복양조세포적형광강도교상양조세포명현감약；상양조급결양/복양조β-catenin단백정위우세포자양세포적포핵급포질중，결양/복양조세포적형광강도교상양조세포명현감약。（6）상양조세포SATB1급β-catenin단백표체수평분별위0.213±0.005화0.797±0.081，이결양/복양조분별위0.083±0.021화0.543±0.131。량조분별비교，차이균유통계학의의（P<0.05）。（7）자간전기조태반조직중화결양/복양조세포중경SATB1항체면역공침정후적단백복합물중，균가검측도β-catenin단백적표체；동양，경β-catenin항체면역공침정후적단백복합물중，야균가검측도SATB1단백적표체。（8）자간전기조태반조직중MMP-2、9활성수평분별위2.251±0.310、1.447±0.102，정상족월조분별위7.098±0.451、5.502±0.197，량조분별비교，차이유통계학의의（P<0.05）。결양/복양조세포중MMP-2、9활성수평분별위0.471±0.104、0.297±0.103，상양조분별위0.842±0.209、0.595±0.100，결양/복양조세포명현저우상양조，분별비교，차이균유통계학의의（P<0.05）。결론자간전기태반조직중SATB1표체수평하강，가능시통과조공wnt/β-catenin신호통로이영향MMP-2、9적활성수평개변，사자양세포적침습력감약，최종도치자간전기적발생。
Objective To explore the role of specific AT rich sequence binding protein 1(SATB1) and wnt/β-catenin signaling pathways in the regulation of trophoblast invasion and its effect in the pathogenesis of preeclampsia. Methods From March 2013 to March 2014, 20 cases of human villous tissues (early pregnancy group) from women of 8-10 gestational weeks who received artificial abortion at the First Affiliated Hospital of Chongqing Medical University, 18 cases of placental tissues (mid-pregnancy group) from women of 18-20 gestational weeks who had labor induction by water bag, 20 cases of placental tissues (normal full-term group) from healthy full-term pregnancy women and 20 cases of placental tissues (preeclamptic group) from women with preeclampsia who received elective c-section in were collected. Immunohistochemical SP method was utilized to determine the position of SATB1 and beta-catenin in villous tissues or placental tissues. Western blot was performed to analyze the expression level of SATB1 and beta-catenin in villous tissues or placental tissues. Immunofluorescence assay was used to determine the location of SATB1 andβ-catenin in HTR8/SVneo cells. Western blot was performed to detect the expression level of SATB1 and beta-catenin in HTR8/SVneo cells cultured in normoxia and hypoxia reoxygenation(H/R) condition. Co-Immunoprecipitation detection was used to evaluate the interaction between SATB1 andβ-catenin in placental tissues in preeclamptic group and HTR8/SVneo cells in H/R group. Gelatin zymography analysis was used to measure the activity of matrix metalloproteinases(MMP)-2 and 9 in placental tissues from preeclamptic group and HTR8/SVneo cells in H/R group. Results (1) In the normal full-term group, rare syncytiotrophoblastic nodule, less fibrinoid necrosis and abundant numbers of capillary could be observed in placental tissues. In comparison, there were obvious vacuolation in the cytoblast of syncytiotrophoblast, rich fibrinoid necrosis and poor numbers of villous capillary in placental tissues from preeclamptic group. (2) SATB1 could be found by immunochemical staining in placenta or villous tissues from all the groups. The staining intensity of SATB1 were more weakening in preeclamptic group than in the normal full-term group. (3) β-catenin could be found by immunochemical staining in placenta or villous tissues from all the groups. The staining intensity of β-catenin were more weakening in preeclamptic group than in the normal full-term group. (4) The protein expression levels of SATB1 in early pregnancy group, mid-pregnancy group, normal full-term group and preeclamptic group were 0.300 ± 0.009, 0.271 ± 0.015, 0.238 ± 0.018 and 0.153 ± 0.007, respectively. The protein levels of β-catenin among the above groups were 0.743±0.041, 0.648±0.021, 0.549±0.069 and 0.269±0.047, respectively. Both the expression of SATB1 andβ-catenin protein were significant decreased in placental tissues from preeclamptic group compared with the other three groups. (5) The SATB1 andβ-catenin protein was located in nucleus of trophoblast and a small amount was in the cytoplasm. The fluorescence intensity of both SATB1 and β-catenin in the H/R group were significantly decreasing when compared to the normoxia group. (6) HTR8/SVneo cells in H/R group showed a significant decrease in both SATB1 andβ-catenin protein levels when compared to the normoxia group. The protein level of SATB1 in the normoxia group was 0.213 ± 0.005, while was 0.083 ± 0.021 in the H/R group. The protein level ofβ-catenin in the normoxia group was 0.797±0.081, and was 0.543±0.131 in the H/R group. (7) There was an interaction between SATB1 and β-catenin in placental tissues from the preeclamptic group and HTR8/SVneo cells exposed by H/R. (8) The enzymatic activity of MMP-2 and MMP-9 protein were decreased significantly in placental tissues from the preeclamptic group (2.251±0.310, 1.447 ± 0.102, respectively) when compared to the normal full-term group (7.098 ± 0.451, 5.502 ± 0.197, respectively). MMP-2 and MMP-9 were significantly decreased in the H/R group (0.471 ± 0.104, 0.297 ± 0.103, respectively) when compared to the normoxia group (0.842 ± 0.209, 0.595 ± 0.100, respectively). Conclusion The expression of SATB1 decreased in the placenta of preeclampsia. This may influence the activity of MMP-2 and 9 by regulating Wnt/β-catenin signaling pathways, affect trophoblast invasion and eventually result in preeclampsia.
