CAJ | 학술논문

目的：探究大黄素对有机磷中毒诱发呼吸衰竭的干预作用。方法60只清洁级雄性Wistar大鼠随机分为：正常对照组，模型对照组，阳性药组及大黄素组，每组各15只。除正常对照组外大鼠均腹腔麻醉，予以右颈总动脉插管，待大鼠清醒开始染毒造模。比较染毒前，呼吸衰竭时，干预5、10、30 min大鼠呼吸频率，血气分析各项指标水平及大鼠血清氧自由基。结果大鼠染毒后出现张口呼吸、呼吸频率减慢等呼吸困难症状，出现紫绀、测动脉血气PaO2＜60 mmHg提示呼吸衰竭模型复制成功。染毒后大鼠呼吸频率下降，与阳性药组比较，大黄素组10、30 min呼吸频率较高（ P＜0.05）；呼吸衰竭时，各组PaO2、SaO2、BE值下降，PaCO2值上升，与阳性药组比较，大黄素组治疗10、30 min PaO2、PaCO2、SaO2以及BE较高，差异具有统计学意义（ P＜0.05）；染毒前，呼吸衰竭时各组大鼠氧自由基水平无统计学差异，与阳性药组比较，大黄素组干预30 min后，SOD及MDA值较高（ P＜0.05）。结论大黄素能够提高有机磷中毒诱发呼吸衰竭机体呼吸频率，改善血气分析各项指标，提高机体氧自由基水平。
목적：탐구대황소대유궤린중독유발호흡쇠갈적간예작용。방법60지청길급웅성Wistar대서수궤분위：정상대조조，모형대조조，양성약조급대황소조，매조각15지。제정상대조조외대서균복강마취，여이우경총동맥삽관，대대서청성개시염독조모。비교염독전，호흡쇠갈시，간예5、10、30 min대서호흡빈솔，혈기분석각항지표수평급대서혈청양자유기。결과대서염독후출현장구호흡、호흡빈솔감만등호흡곤난증상，출현자감、측동맥혈기PaO2＜60 mmHg제시호흡쇠갈모형복제성공。염독후대서호흡빈솔하강，여양성약조비교，대황소조10、30 min호흡빈솔교고（ P＜0.05）；호흡쇠갈시，각조PaO2、SaO2、BE치하강，PaCO2치상승，여양성약조비교，대황소조치료10、30 min PaO2、PaCO2、SaO2이급BE교고，차이구유통계학의의（ P＜0.05）；염독전，호흡쇠갈시각조대서양자유기수평무통계학차이，여양성약조비교，대황소조간예30 min후，SOD급MDA치교고（ P＜0.05）。결론대황소능구제고유궤린중독유발호흡쇠갈궤체호흡빈솔，개선혈기분석각항지표，제고궤체양자유기수평。
Objective To explore the intervention effect of emodin on organophosphorus poisoning induced respiratory failure.Methods 60 male Wistar rats of clean grade were randomly divided into:normal control group, model control group, positive drug group and emodin group, with 15 rats in each group.Except the normal control group rats were given intraperitoneal anesthesia, the right common carotid artery intubation, when rats stayed awake began a septic model.Blood gas analysis and serum level of oxygen free radicals and respiratory rate were compared before poisoning, respiratory failure, intervention of 5, 10, 30 min.Results Mouth breathing, slow respiratory frequency and cyanosis, appeared after exposure.Respiratory frequency decreased after exposure , compared with the positive drug group, respiratory frequency of emodin group 10 min and 30 min was higher ( P<0.05), PaO2, SaO2, BE decreased, PaCO2 increased after respiratory failure, Compared with the positive drug group, PaO2, PaCO2, SaO2 and BE of emodin group for the treatment of 10 min, 30 min was higher,(P <0.05).The level of oxygen free radicals in rats of each group had no significant difference before the exposure and the respiratory failure.Compared with the positive drug group, SOD and MDA of emodin group in 30 min after intervention were higher,( P<0.05 ) .Conclusion Emodin can improve the respiratory frequency of organic phosphorus poisoning induced respiratory failure ,improve blood gas analysis of the indicators and the level of oxygen free radicals.