中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2015年
9期
1608-1612
,共5页
结肠炎%内质网应激%葡萄糖调节蛋白78%葡聚糖硫酸钠
結腸炎%內質網應激%葡萄糖調節蛋白78%葡聚糖硫痠鈉
결장염%내질망응격%포도당조절단백78%포취당류산납
Colitis%Endoplasmic reticulum stress%Glucose regulated protein 78%Dextran sulphate sodium
目的：探讨内质网应激标志蛋白GRP78在小鼠实验性结肠炎组织中的表达情况。方法将8周龄C57BL/6小鼠随机分为对照组、葡聚糖硫酸钠（DSS）组，每组7只，对照组正常饮水，DSS组饮用含2.5% DSS的饮水，共6 d建立小鼠急性结肠炎模型。通过疾病活动指数评分（DAI）、测量结肠长度、HE染色病理评分、炎性指标TNFα和IL-6 mRNA检测来评估结肠炎症损伤程度。采用免疫组织化学法和实时定量PCR法检测结肠组织中GRP78蛋白和mRNA表达情况。结果对照组未见肠炎表现，DSS组小鼠出现典型结肠炎表现。DSS组炎性指标 TNFα和IL-6 mRNA均较对照组显著升高。GRP78蛋白和mRNA在对照组高表达，在DSS 组表达显著减少（P＜0.05）。结论 GRP78在维持肠道正常生理功能中发挥重要作用，炎症状态下其表达受抑制。
目的：探討內質網應激標誌蛋白GRP78在小鼠實驗性結腸炎組織中的錶達情況。方法將8週齡C57BL/6小鼠隨機分為對照組、葡聚糖硫痠鈉（DSS）組，每組7隻，對照組正常飲水，DSS組飲用含2.5% DSS的飲水，共6 d建立小鼠急性結腸炎模型。通過疾病活動指數評分（DAI）、測量結腸長度、HE染色病理評分、炎性指標TNFα和IL-6 mRNA檢測來評估結腸炎癥損傷程度。採用免疫組織化學法和實時定量PCR法檢測結腸組織中GRP78蛋白和mRNA錶達情況。結果對照組未見腸炎錶現，DSS組小鼠齣現典型結腸炎錶現。DSS組炎性指標 TNFα和IL-6 mRNA均較對照組顯著升高。GRP78蛋白和mRNA在對照組高錶達，在DSS 組錶達顯著減少（P＜0.05）。結論 GRP78在維持腸道正常生理功能中髮揮重要作用，炎癥狀態下其錶達受抑製。
목적：탐토내질망응격표지단백GRP78재소서실험성결장염조직중적표체정황。방법장8주령C57BL/6소서수궤분위대조조、포취당류산납（DSS）조，매조7지，대조조정상음수，DSS조음용함2.5% DSS적음수，공6 d건립소서급성결장염모형。통과질병활동지수평분（DAI）、측량결장장도、HE염색병리평분、염성지표TNFα화IL-6 mRNA검측래평고결장염증손상정도。채용면역조직화학법화실시정량PCR법검측결장조직중GRP78단백화mRNA표체정황。결과대조조미견장염표현，DSS조소서출현전형결장염표현。DSS조염성지표 TNFα화IL-6 mRNA균교대조조현저승고。GRP78단백화mRNA재대조조고표체，재DSS 조표체현저감소（P＜0.05）。결론 GRP78재유지장도정상생리공능중발휘중요작용，염증상태하기표체수억제。
Objective To investigate the expression of ER stress marker protein GRP78 in experimental colitis mice. Methods The 8-week-old C57BL/6 mice were randomly divided into control group and Dextran sulfate sodium (DSS) group. n=7 for each group, mice in control group was given with only drinking water, acute colitis model was established by given with drinking water dissolved with 2.5%DSS to DSS group for 6 days. Disease Activity Index (DAI), colon length and inflammatory scores of colon were observed, inflammatory indicators were measured. The protein and mRNA expressions of GRP78 in colon tissue of mice were evaluated by immunohistochemistry and quantitative real-time PCR respectively. Results There was no colitis in control group, severe colitis were found in DSS group. Inflammatory indicators TNFα and IL-6 mRNA were increased significantly compared to the control group. GRP78 protein and mRNA was highly expressed in normal colon tissues, while in DSS group reduced significantly. Conclusion GRP78 is mainly involved in the functional and structural maintenance of normal colonic epithelium, expression is suppressed under inflammatory condition.
