龙岩学院学报
龍巖學院學報
룡암학원학보
JOURNAL OF LONGYAN UNIVERSITY
2015年
2期
89-93
,共5页
孔祥海%邱丰艳%丁力%陈小红
孔祥海%邱豐豔%丁力%陳小紅
공상해%구봉염%정력%진소홍
富贵籽%茎段%腋芽%诱导%增殖%试管苗
富貴籽%莖段%腋芽%誘導%增殖%試管苗
부귀자%경단%액아%유도%증식%시관묘
Ardisia crenata%stem segments%axillary buds%induction%growth and proliferation%rooting%test tube plantlets
以不同植物激素组合和不同发育阶段的茎段为实验因子,开展富贵籽茎段诱导腋芽生长及其增殖和试管苗培育的实验研究。结果:(1) MS + NAA 0?1 mg·L-1+ BA 0?50 mg·L-1的培养基配方较适合于从富贵籽无菌苗和当年萌发的嫩枝茎段诱导腋芽生长与增殖;(2) MS+NAA 0?1 mg·L-1+BA 1?50 mg·L-1的组合配方能较好的诱导富贵籽1年生茎段腋芽生长与增殖。(3)1/2MS+ NAA 0?50 mg·L-1+IBA 0?30 mg·L-11的组合配方用于不定芽体诱导生根和培育试管苗的效果最好。
以不同植物激素組閤和不同髮育階段的莖段為實驗因子,開展富貴籽莖段誘導腋芽生長及其增殖和試管苗培育的實驗研究。結果:(1) MS + NAA 0?1 mg·L-1+ BA 0?50 mg·L-1的培養基配方較適閤于從富貴籽無菌苗和噹年萌髮的嫩枝莖段誘導腋芽生長與增殖;(2) MS+NAA 0?1 mg·L-1+BA 1?50 mg·L-1的組閤配方能較好的誘導富貴籽1年生莖段腋芽生長與增殖。(3)1/2MS+ NAA 0?50 mg·L-1+IBA 0?30 mg·L-11的組閤配方用于不定芽體誘導生根和培育試管苗的效果最好。
이불동식물격소조합화불동발육계단적경단위실험인자,개전부귀자경단유도액아생장급기증식화시관묘배육적실험연구。결과:(1) MS + NAA 0?1 mg·L-1+ BA 0?50 mg·L-1적배양기배방교괄합우종부귀자무균묘화당년맹발적눈지경단유도액아생장여증식;(2) MS+NAA 0?1 mg·L-1+BA 1?50 mg·L-1적조합배방능교호적유도부귀자1년생경단액아생장여증식。(3)1/2MS+ NAA 0?50 mg·L-1+IBA 0?30 mg·L-11적조합배방용우불정아체유도생근화배육시관묘적효과최호。
Ardisia crenata is important ornamental and medicinal plants. The experimental effect research of the different combinations of plant hormones on induced growth and proliferation axillary buds of aseptic stems, yearly germinating shoots stems and one-year-old stem of perennial plants were carried out. The results were as follows:The medium formula of MS+NAA 0.1 mg· L-1( the same as below) + BA 0. 50 was suitable for induced growth and proliferation axillary buds of aseptic stems and yearly germinating shoots stems, while the medium formula of MS + NAA 0.10 + BA 1.50 was suitable for induced growth and proliferation axillary buds of 1-year old stems. The combination formula of 1/2 MS + NAA 0.50 +IBA 0.30 had best effect for adventitious bud rooting and the cultivation of test tube plantlets.