CAJ | 학술논문

铁观音茶树不同生育时期叶片为材料，采用Tripure试剂法、申能博彩试剂盒与Tripure试剂相结合的方法提取茶树叶片总RNA。结果表明，Tripure试剂法比结合法更适宜提取铁观音茶树RNA，该方法提取的RNA 28S、18S和5S rRNA条带清晰明亮；OD260／OD280值在1?7～2?0之间，说明该方法提取的RNA完整性和纯度好，可用于进行后续分子生物学实验。
철관음다수불동생육시기협편위재료，채용Tripure시제법、신능박채시제합여Tripure시제상결합적방법제취다수협편총RNA。결과표명，Tripure시제법비결합법경괄의제취철관음다수RNA，해방법제취적RNA 28S、18S화5S rRNA조대청석명량；OD260／OD280치재1?7～2?0지간，설명해방법제취적RNA완정성화순도호，가용우진행후속분자생물학실험。
In order to approach an effective method of total RNA extraction from Tieguanyin tea plant leaves at different developing stages, two methods ( Tripure method and Shennengbocai kit & Tripure combining method ) were used in this experiment. The result indicated that Tripure method was more suitable than Shennengbocai kit & Tripure combining method in the extraction of total RNA from tea plants. The total RNA extracted by Tripure method showed clear and bright bands of 28S, 18S and 5SrRNA. At the same time, the OD260/OD280 absorbance ratio is between 1.7 and 2.0, suggesting the integrity and purity of RNA isolated are good and the RNA can be used for further molecular biology experiments.