CAJ | 학술논문

近年来，分析工作者采用超高效液相色谱（ UPLC）完成了许多过去不能完成的分离分析工作。但是在阐述UPLC原理时不少人却采用了 van Deemter方程。这是不对的。本文研究了 UPLC 色谱过程动力学，从热传导方程出发运用色谱动力学原理推导了包括考虑流动相摩擦生热影响的 UPLC 塔板高度方程 H =2γD m／u+2λdpu1／3u1／3+ω（Dm／dp）1／3+2ku（1+k）2（1+κ0）kd +30θ（κ0+κ0k+k）2d2puDmκ0（1+κ0）2（1+k）2+κi（κ0+κ0k+k）2d5／3p u2／33κ0ΩD2／3m（1+κ0）2（1+k）2+ r20（κ0+κ0k+k）u4（1+k）Dr · exp（-Kr20α）。上述方程右端最后一项代表了流动相摩擦生热对塔板高度的贡献。当流动相线速度较低时，流动相摩擦生热对塔板高度的贡献趋近于零，塔板高度方程还原成 Horvath 和 Lin 的方程；当流动相线速度较高时，由于流动相摩擦生热，柱轴心与边缘温差增加，流动相线速度径向分布差异导致柱效率降低，而柱轴心与边缘的温差与流动相线速度平方成正比。作者明确指出：UPLC 的柱效率与柱内径密切相关，采用细内径柱有利于实现高效率；过高的流动相线速度将导致柱效率崩溃。
근년래，분석공작자채용초고효액상색보（ UPLC）완성료허다과거불능완성적분리분석공작。단시재천술UPLC원리시불소인각채용료 van Deemter방정。저시불대적。본문연구료 UPLC 색보과정동역학，종열전도방정출발운용색보동역학원리추도료포괄고필류동상마찰생열영향적 UPLC 탑판고도방정 H =2γD m／u+2λdpu1／3u1／3+ω（Dm／dp）1／3+2ku（1+k）2（1+κ0）kd +30θ（κ0+κ0k+k）2d2puDmκ0（1+κ0）2（1+k）2+κi（κ0+κ0k+k）2d5／3p u2／33κ0ΩD2／3m（1+κ0）2（1+k）2+ r20（κ0+κ0k+k）u4（1+k）Dr · exp（-Kr20α）。상술방정우단최후일항대표료류동상마찰생열대탑판고도적공헌。당류동상선속도교저시，류동상마찰생열대탑판고도적공헌추근우령，탑판고도방정환원성 Horvath 화 Lin 적방정；당류동상선속도교고시，유우류동상마찰생열，주축심여변연온차증가，류동상선속도경향분포차이도치주효솔강저，이주축심여변연적온차여류동상선속도평방성정비。작자명학지출：UPLC 적주효솔여주내경밀절상관，채용세내경주유리우실현고효솔；과고적류동상선속도장도치주효솔붕궤。
In recent years,separation and analysis workers have made considerable progress on ultra performance liquid chromatography( UPLC). A lot of separation works have been com-pleted which have never been done before. But the theory about chromatographic dynamics of UPLC from the beginning up to now,is remaining in 1950’s. Some people explain the principle of UPLC by the van Deemter equation,but this is not right. In this paper,the dynamic process of UPLC has been studied. According to the chromatographic dynamics principle and starting from the heat conduction equation,the plate height equation of UPLC including the influence of mobile phase friction heat production has been deduced as follows: H = 2γD m/u +2λdpu1/3u1/3+ω(Dm/dp)1/3 + 2ku(1+k)2(1+κ0)kd + 30θ(κ0+κ0k+k)2d2puDmκ0(1+κ0)2(1+k)2 + κi(κ0+κ0k+k)2d5/3p u2/33κ0ΩD2/3m(1+κ0)2(1+k)2 +r20(κ0+κ0k+k)u 4(1+k)Dr exp(-Kr20α). The last item on the right represents the contribution of mobile phase friction heat. When the linear velocity of mobile phase is lower,the contribution of mo-bile phase friction heat tending to become zero,the plate height equation is reduced to the Hor-vath and Lin equation. When the linear velocity of mobile phase is high,the temperature differ-ence between the column axial center and edge is directly proportional to the mobile phase line-ar speed square,and the contribution of thermal effect will largely increased. In this paper,the author clearly pointed out that the column efficiency of UPLC has a direct bearing on the col-umn diameter. Using fine diameter column is helpful to implement column efficiency. Too high mobile phase velocity will lead to the column efficiency collapsed.