江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2015年
2期
314-317
,共4页
李海琴%刘林秀%季华员%武艳平%康昭风
李海琴%劉林秀%季華員%武豔平%康昭風
리해금%류림수%계화원%무염평%강소풍
地方品种鸡%禽白血病病毒%J-亚群%感染%净化
地方品種鷄%禽白血病病毒%J-亞群%感染%淨化
지방품충계%금백혈병병독%J-아군%감염%정화
local chicken breeds%avian leucosis virus%ALV-J%infection%eradication
为了解江西省地方品种鸡群中禽白血病病毒的感染状况,利用针对禽白血病病毒( ALV) J亚群抗体和群特异性P27抗原的商品化ELISA检测试剂盒,对江西省12个种鸡企业的6个地方品种鸡进行禽白血病感染情况调查。结果显示江西几个地方品种鸡ALV的感染相当严重,迫切需要净化。但不同品种鸡的P27抗原的检出率与ALV-J抗体的检出率有一定差异。 P27抗原阳性率最高的为JXDF4品种鸡(36.67%),其次为JXDF5、JXDF1、JX?DF3、JXDF6、JXDF2品种鸡。与P27抗原检出情况类似,JXDF4、JXDF5的ALV-J抗体阳性率相对较高,分别为15%、30%,而其他4个地方品种的鸡群ALV-J抗体阳性率相对较低(≦10%)。调查还显示,同一品种鸡在不同种鸡场之间的ALV-J感染率差异较大。试验结果为各种鸡场开展净化工作提供了科学依据。
為瞭解江西省地方品種鷄群中禽白血病病毒的感染狀況,利用針對禽白血病病毒( ALV) J亞群抗體和群特異性P27抗原的商品化ELISA檢測試劑盒,對江西省12箇種鷄企業的6箇地方品種鷄進行禽白血病感染情況調查。結果顯示江西幾箇地方品種鷄ALV的感染相噹嚴重,迫切需要淨化。但不同品種鷄的P27抗原的檢齣率與ALV-J抗體的檢齣率有一定差異。 P27抗原暘性率最高的為JXDF4品種鷄(36.67%),其次為JXDF5、JXDF1、JX?DF3、JXDF6、JXDF2品種鷄。與P27抗原檢齣情況類似,JXDF4、JXDF5的ALV-J抗體暘性率相對較高,分彆為15%、30%,而其他4箇地方品種的鷄群ALV-J抗體暘性率相對較低(≦10%)。調查還顯示,同一品種鷄在不同種鷄場之間的ALV-J感染率差異較大。試驗結果為各種鷄場開展淨化工作提供瞭科學依據。
위료해강서성지방품충계군중금백혈병병독적감염상황,이용침대금백혈병병독( ALV) J아군항체화군특이성P27항원적상품화ELISA검측시제합,대강서성12개충계기업적6개지방품충계진행금백혈병감염정황조사。결과현시강서궤개지방품충계ALV적감염상당엄중,박절수요정화。단불동품충계적P27항원적검출솔여ALV-J항체적검출솔유일정차이。 P27항원양성솔최고적위JXDF4품충계(36.67%),기차위JXDF5、JXDF1、JX?DF3、JXDF6、JXDF2품충계。여P27항원검출정황유사,JXDF4、JXDF5적ALV-J항체양성솔상대교고,분별위15%、30%,이기타4개지방품충적계군ALV-J항체양성솔상대교저(≦10%)。조사환현시,동일품충계재불동충계장지간적ALV-J감염솔차이교대。시험결과위각충계장개전정화공작제공료과학의거。
To investigate the status of avian leukosis virus( ALV) infection in Jiangxi local chicken breeds, 6 local chicken breeds from 12 poultry enterprises in Jiangxi Province were tested by two commercial kits for the detection of antibodies against subgroup J,and the group specific antigen P27.The results showed that the infection of ALV was very serious in these tested breeds needing urgent purification,but there were some differ?ences in ALV antigen detection rate and ALV?J antibody detection rate among different breeds,the ALV anti?gen positive of JXDF4 was the highest(36.67%),followed by JXDF5,JXDF1,JXDF3,JXDF6,JXDF2.Same as the situation of ALV antigen detection rate,the antibody detection rates of JXDF4,JXDF5 (15.5%,30%)were higher than those of the other four breeds(≦10%) . The results also showed that there were significant differ?ences in ALV?J antigen detection rate in the same breeds in different fields.The results could be helpful for e?radication program.