江西农业大学学报
江西農業大學學報
강서농업대학학보
ACTA AGRICULTURAE UNIVERSITATIS JIANGXIENSIS
2015年
2期
308-313
,共6页
黄江南%刘林秀%武艳平%康昭风%韦启鹏%周泉勇%季华员%曾文%陈受金%谢金防
黃江南%劉林秀%武豔平%康昭風%韋啟鵬%週泉勇%季華員%曾文%陳受金%謝金防
황강남%류림수%무염평%강소풍%위계붕%주천용%계화원%증문%진수금%사금방
兴国灰鹅%光照%产蛋%催乳素%促卵泡素β%促黄体素
興國灰鵝%光照%產蛋%催乳素%促卵泡素β%促黃體素
흥국회아%광조%산단%최유소%촉란포소β%촉황체소
Xingguo grey geese%light exposure%egg production%PRL%FSHβ%LH
为研究光照时间对兴国灰鹅产蛋量及产蛋性状相关基因在下丘脑－垂体－性腺轴表达水平的影响，对兴国灰鹅分别进行长光照处理（试验组）和自然光照处理（对照组）。结果发现，整个试验阶段，对照组持续产蛋，产蛋率维持在10％以上，处理组在接受递增式长光照至40 d左右时，产蛋率下降到8％左右，并于试验第47天停产。基因表达水平方面，相对于对照组产蛋期鹅，试验组鹅下丘脑中催乳素（ PRL）的mRNA表达水平与输卵管和卵巢中促卵泡素（ FSHβ）的表达水平均显著升高，而卵巢中黄体生成素（ LH）的mRNA表达水平显著降低。这些结果表明长光照可通过影响 PRL的mRNA表达，从而导致FSHβ和LH表达比例失调，最终影响FSHβ和LH的协同作用，从而抑制兴国灰鹅的产蛋活动。
為研究光照時間對興國灰鵝產蛋量及產蛋性狀相關基因在下丘腦－垂體－性腺軸錶達水平的影響，對興國灰鵝分彆進行長光照處理（試驗組）和自然光照處理（對照組）。結果髮現，整箇試驗階段，對照組持續產蛋，產蛋率維持在10％以上，處理組在接受遞增式長光照至40 d左右時，產蛋率下降到8％左右，併于試驗第47天停產。基因錶達水平方麵，相對于對照組產蛋期鵝，試驗組鵝下丘腦中催乳素（ PRL）的mRNA錶達水平與輸卵管和卵巢中促卵泡素（ FSHβ）的錶達水平均顯著升高，而卵巢中黃體生成素（ LH）的mRNA錶達水平顯著降低。這些結果錶明長光照可通過影響 PRL的mRNA錶達，從而導緻FSHβ和LH錶達比例失調，最終影響FSHβ和LH的協同作用，從而抑製興國灰鵝的產蛋活動。
위연구광조시간대흥국회아산단량급산단성상상관기인재하구뇌－수체－성선축표체수평적영향，대흥국회아분별진행장광조처리（시험조）화자연광조처리（대조조）。결과발현，정개시험계단，대조조지속산단，산단솔유지재10％이상，처리조재접수체증식장광조지40 d좌우시，산단솔하강도8％좌우，병우시험제47천정산。기인표체수평방면，상대우대조조산단기아，시험조아하구뇌중최유소（ PRL）적mRNA표체수평여수란관화란소중촉란포소（ FSHβ）적표체수평균현저승고，이란소중황체생성소（ LH）적mRNA표체수평현저강저。저사결과표명장광조가통과영향 PRL적mRNA표체，종이도치FSHβ화LH표체비례실조，최종영향FSHβ화LH적협동작용，종이억제흥국회아적산단활동。
To examine the effect of long illumination on the egg production and expression level of PRL, FSHβ and LH in the hypothalamus?pituitary?gonadal axis of Xingguo grey geese,adult breeding geese were ei?ther exposed to natural photoperiod (controls),or subjected to treatments of long photoperiod throughout the experient.The results showed that,the laying rate remained above 10% in the normal illumination group,and the egg laying of treated geese quickly decreased after 40?day treatment of long illumination and ceased on day 47.The mRNA expression level of prolactin (PRL) in the hypothalamus of the long illumination group was sig?nificantly higher than that in the normal illumination group.The mRNA expression level of follicle stimulating hormone ( FSHβ) in the oviduct and ovaries of long illumination group was significantly higher than that in the normal illumination group. The mRNA expression level of luteinizing hormone ( LH) of the long illumination group was significantly lower than that in the normal illumination group. These results indicate that the in?creased expression of PRL and the imbalance of FSHβ/LH ratio may be involved in the suppressing of the re?productive activity of Xingguo grey geese under long term illumination.
