CAJ | 학술논문

本研究以波尔山羊为实验动物，旨在观察钼镉联合胁迫对山羊肾脏细胞凋亡相关基因表达的影响。选用63头健康波尔山羊随机分成7组，每组3个重复，选用七钼酸铵（[（NH4）6Mo7O24·4H2O]）作为实验钼源，氯化镉（CdCl2）作为实验镉源，按每kg山羊体质量添加钼、镉对照组（Mo 0 mg／kg＋Cd 0 mg／kg），低镉低钼组（Mo 15 mg／kg＋Cd 0．5 mg／kg），低镉中钼组（ Mo 30 mg／kg＋Cd 0．5 mg／kg），低镉高钼组（ Mo 45 mg／kg＋Cd 0．5 mg／kg），高镉低钼组（Mo 15 mg／kg＋Cd 1．0 mg／kg），高镉中钼组（Mo 30 mg／kg＋Cd 1．0 mg／kg），高镉高钼组（Mo 45 mg／kg＋Cd 1．0 mg／kg），实验期50 d。并于当天、第25、50天每组各剖杀3头山羊取肾组织进行相关实验，观察不同剂量钼、镉对山羊肾脏细胞凋亡相关基因表达等的影响。结果显示，与对照组相比，在实验第25、50天时，实验组山羊肾脏bcl－2 mRNA 表达量下降（P＜0．01），bax mRNA、caspase－3 mRNA 和 cytc mRNA 表达量上升（P＜0．05或P＜0．01）；山羊钼镉联合胁迫导致肾线粒体抗氧化功能降低、自由基含量升高，导致细胞促凋亡基因表达量上升，抗凋亡基因的表达量下降，提示钼镉联合胁迫对山羊肾脏有明显损伤。
본연구이파이산양위실험동물，지재관찰목력연합협박대산양신장세포조망상관기인표체적영향。선용63두건강파이산양수궤분성7조，매조3개중복，선용칠목산안（[（NH4）6Mo7O24·4H2O]）작위실험목원，록화력（CdCl2）작위실험력원，안매kg산양체질량첨가목、력대조조（Mo 0 mg／kg＋Cd 0 mg／kg），저력저목조（Mo 15 mg／kg＋Cd 0．5 mg／kg），저력중목조（ Mo 30 mg／kg＋Cd 0．5 mg／kg），저력고목조（ Mo 45 mg／kg＋Cd 0．5 mg／kg），고력저목조（Mo 15 mg／kg＋Cd 1．0 mg／kg），고력중목조（Mo 30 mg／kg＋Cd 1．0 mg／kg），고력고목조（Mo 45 mg／kg＋Cd 1．0 mg／kg），실험기50 d。병우당천、제25、50천매조각부살3두산양취신조직진행상관실험，관찰불동제량목、력대산양신장세포조망상관기인표체등적영향。결과현시，여대조조상비，재실험제25、50천시，실험조산양신장bcl－2 mRNA 표체량하강（P＜0．01），bax mRNA、caspase－3 mRNA 화 cytc mRNA 표체량상승（P＜0．05혹P＜0．01）；산양목력연합협박도치신선립체항양화공능강저、자유기함량승고，도치세포촉조망기인표체량상승，항조망기인적표체량하강，제시목력연합협박대산양신장유명현손상。
This experiment was conducted to determine the effect of combining stress of molybdenum and cadmium on the expressions of apoptosis related genes in the kidney of goat.63 goats were randomly assigned into seven different groups, and three repeated of each group. [ ( NH4 ) 6 Mo7 O24 · 4H2 O ] was used as the source of molybdenum,and CdCl2 as the source of cadmium.The treatments consisted of the control group (Mo 0 mg/kg+Cd 0 mg/kg),the low cadmium?low molybdenum group (Mo 15 mg/kg+Cd 0.5 mg/kg),the low cad?mium?middle molybdenum group ( Mo 30 mg/kg+Cd 0. 5 mg/kg ) , the low cadmium?high molybdenum group ( Mo 45 mg/kg+Cd 0.5 mg/kg) ,the high cadmium?low molybdenum group ( Mo 15 mg/kg+Cd 1.0 mg/kg) ,the high cadmium?middle molybdenum group ( Mo 30 mg/kg+Cd 1.0 mg /kg) and the high cadmium?high mo?lybdenum group (Mo 45 mg/kg+Cd 1.0 mg/kg) for 50 days under normal condition.Kidney tissue samples were collected on days 0,25 and 50 to determine the activities of the expressions of apoptosis related genes in the kidney.Compared with those of the control group,the expressions of bcl?2 mRNA decreased (P<0.01),the expressions of bax mRNA,caspase?3 mRNA and cytc mRNA increased( P<0.05 and P<0.01) in the experi?mental groups on the 25th and 50th day.The results indicated that the combined stree of molybdenum and cad?mium could increase the expressions of promote apoptosis genes and decrease the expressions of anti?apoptosis genes in the kidney of goat,which suggests that the combined stress of molybdenum and cadmium causes sig?nificant damages in the kidney of goat.