CAJ | 학술논문

应用ISSR标记对山西省7个野生居群和1个人工栽培居群共60份西伯利亚远志种质进行遗传多样性分析。结果表明：从100对ISSR引物中共筛选出20条多态性好、条带稳定的引物，60份材料DNA共获得338个扩增位点，其中多态性位点334个，多态性比率为98.82%；有效等位基因数(Ne )、 Nei's基因多样性指数(H)及Shannon多样性信息指数(I)分别为1.4337、0.2656和0.4142，居群的遗传相似度在0.6552～0.9779，表明8个居群具有丰富的遗传多样性，这与其自身繁殖特点有关；遗传分化系数(Gst)和基因流(Nm)分别为0.5260和0.4505，表明居群内和居群间均有一定程度的分化，基因流水平较低；利用UPGMA法进行聚类分析，将8个居群划分为3类。研究结果为合理引种、驯化、保护和利用西伯利亚远志野生资源提供了重要的参考依据和数据支持。
응용ISSR표기대산서성7개야생거군화1개인공재배거군공60빈서백리아원지충질진행유전다양성분석。결과표명：종100대ISSR인물중공사선출20조다태성호、조대은정적인물，60빈재료DNA공획득338개확증위점，기중다태성위점334개，다태성비솔위98.82%；유효등위기인수(Ne )、 Nei's기인다양성지수(H)급Shannon다양성신식지수(I)분별위1.4337、0.2656화0.4142，거군적유전상사도재0.6552～0.9779，표명8개거군구유봉부적유전다양성，저여기자신번식특점유관；유전분화계수(Gst)화기인류(Nm)분별위0.5260화0.4505，표명거군내화거군간균유일정정도적분화，기인류수평교저；이용UPGMA법진행취류분석，장8개거군화분위3류。연구결과위합리인충、순화、보호화이용서백리아원지야생자원제공료중요적삼고의거화수거지지。
The genetic diversity of the sixty samples of Polygala sibirica L. (including seven natural populations and one cultivated population from Shanxi province) was analyzed by ISSR markers. The results showed that 20 effective primer pairs were selected to amplify 60 Polygala sibirica L. individuals. A total of 338 fragments were obtained by amplification of ISSR, and 334 among which were polymorphic, accounting for 98.82% of the total. The effective number of alleles (Ne ), Nei's genetic index(H) and Shannon information index(I) were 1.433 7、 0.265 6 and 0.414 2 respectively, and the genetic similarities of 8 populations were between 0.655 2~0.977 9. The results showed that genetic diversity of 8 populations was high, due to its reproductive features. The genetic differentiation and the gene flow (Nm)among populations (Gst) was 0.526 0 and 0.450 5 respectively, indicating that the genetic differentiations among populations and within populations were both high, whereas the gene flow among populations was low. Finally, the 8 populations of Polygala sibirica L. were clustered into 3 groups using UPGMA. The results of this research provided theoretical and data evidence for the introduction, domestication, conservation and exploitation of wild P. sibirica L. germplasm resources.