CAJ | 학술논문

目的：研究DC-CIK细胞和不同浓度顺铂对乳腺癌MCF-7细胞的增殖抑制作用。方法将DC细胞和CIK细胞共同培养得到的DC-CIK细胞与顺铂分别作用于MCF-7细胞，共同培养12h、24 h和48 h，MTT法检测MCF-7细胞的增殖抑制情况，对比DC-CIK细胞和顺铂对MCF-7细胞的增殖抑制效果。结果与DC-CIK细胞共培养12 h、24 h与48 h，MCF-7细胞均出现一定的凋亡，增殖受到明显的抑制；与5×106个DC-CIK细胞共培养12h与40 ng/mL顺铂对其作用12 h的抑制效果相同，共培养24 h与50 ng/mL顺铂对其作用24 h的效果相同，共培养48 h与60 ng/mL顺铂对其作用24 h的效果相同。结论该研究为DC-CIK法治疗乳腺癌作出了实验室基础性研究，为临床DC-CIK细胞抑制乳腺癌细胞提供了理论依据。
목적：연구DC-CIK세포화불동농도순박대유선암MCF-7세포적증식억제작용。방법장DC세포화CIK세포공동배양득도적DC-CIK세포여순박분별작용우MCF-7세포，공동배양12h、24 h화48 h，MTT법검측MCF-7세포적증식억제정황，대비DC-CIK세포화순박대MCF-7세포적증식억제효과。결과여DC-CIK세포공배양12 h、24 h여48 h，MCF-7세포균출현일정적조망，증식수도명현적억제；여5×106개DC-CIK세포공배양12h여40 ng/mL순박대기작용12 h적억제효과상동，공배양24 h여50 ng/mL순박대기작용24 h적효과상동，공배양48 h여60 ng/mL순박대기작용24 h적효과상동。결론해연구위DC-CIK법치료유선암작출료실험실기출성연구，위림상DC-CIK세포억제유선암세포제공료이론의거。
Objective To investigate the in-vitro inhibitory efects of DC(dendritic cel1)-CIK (cytokine-induced killer cel1)cocul-tured cells and cis-platinum against breast cancer cell line MCF-7 cells. Methods In this study, the DC cells and CIK cells co-cultured DC-CIK cells and in MCF-7 cells co-cultured for 12, 24 and 48 hours, using the MTT assay MCF-7 cell proliferation inhibition and using different concentrations the chemotherapy drug cisplatin role in MCF-7 cells, using the same MTT assay MCF-7 cell proliferation inhibition. Then compare the inhibitory effect of DC-CIK cells and the chemotherapy drug cisplatin pro-liferation of MCF-7 cells. Results Addition of DC-CIK cells using MTT assay DC-CIK role in the MCF-7 cell proliferation of MCF-7 before and after the show, regardless of the role of 12, 24 or 48 hours, MCF-7 cells apoptosis, proliferation was significant-ly inhibited. DC-CIK and cisplatin on MCF-7 proliferation inhibition comparison, 5 ×106 cells proliferation of MCF-7 cells 12h inhibition with 40 ng/mL concentration of cisplatin on the proliferation of MCF-7 cells for 12h inhibition equivalent for 24h inhib-ited with 50 ng/mL cisplatin equivalent to the inhibition of proliferation of MCF-7 cells 24h, 48h role in the inhibition of 60 ng /ml of cisplatin on the proliferation of MCF-7 cells the 24h of inhibition basic equivalent. The results show that the DC-CIK cells to inhibit the proliferation of MCF-7 cells. Conclusion This study DC-CIK therapy for breast cancer made a laboratory for basic research. In the clinical treatment of breast cancer has a theoretical and experimental basis.