CAJ | 학술논문

目的：探讨 Alport 综合征（AS）差异性表达蛋白质主要参与的 GO 富集分析和 KEGG 通路，为 AS 的进一步机制研究奠定基础。方法：10例 AS 患者与10例健康对照者（NC）作为实验对象。分别收集实验参与者尿液200 mL，并从尿液中分离尿肾脏管细胞，将尿肾脏管细胞诱导分化成多潜能干细胞（iPSCs），运用 iTRAQ 技术找出2组之间差异性表达蛋白质，对其进行生物信息学分析。结果：在2组样本中发现35种蛋白质具有差异性表达，包括18种上调与17种下调。差异性表达蛋白质 GO 富集主要聚集在细胞分子、细胞组成与细胞生物学过程。嘌呤代谢通路与丝裂原激活的蛋白激酶是主要的 KEGG 信号传导通路。结论：差异性表达蛋白质的生物信息学有助于 AS发病机制研究，可作为机制研究参考和补充。
목적：탐토 Alport 종합정（AS）차이성표체단백질주요삼여적 GO 부집분석화 KEGG 통로，위 AS 적진일보궤제연구전정기출。방법：10례 AS 환자여10례건강대조자（NC）작위실험대상。분별수집실험삼여자뇨액200 mL，병종뇨액중분리뇨신장관세포，장뇨신장관세포유도분화성다잠능간세포（iPSCs），운용 iTRAQ 기술조출2조지간차이성표체단백질，대기진행생물신식학분석。결과：재2조양본중발현35충단백질구유차이성표체，포괄18충상조여17충하조。차이성표체단백질 GO 부집주요취집재세포분자、세포조성여세포생물학과정。표령대사통로여사렬원격활적단백격매시주요적 KEGG 신호전도통로。결론：차이성표체단백질적생물신식학유조우 AS발병궤제연구，가작위궤제연구삼고화보충。
Aim:To explore the different expression proteins in Alport syndrome(AS)and mainly par-ticipated in the Gene Ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG) pathways,which lay the foundation for further research of mechanism in patients with AS.Methods:The participants in this research consist of 10 AS patients and 10 healthy control (NC).Urine from AS pa-tients and NC were collected to separate the renal tubular cells,which were reprogrammed to generate hu-man induced pluripotent stem cells (iPSCs).The different expression proteins were found by means of i-sobaric tags for relative and absolute quantitation(iTRAQ)technology,and then bioinformatic analysis of these different expressed proteins.Results:Thirty-five proteins were identified to be different expressed (18 were up-regulated and 17 were down-regulated).The different expression proteins mainly enriched in cell molecular,cell part and cellular process.For the KEGG pathway,the different expression proteins mainly participated in puine metabolism and mitogen activated protein kinase(MAPK)signaling pathway. Conclusion:These data suggest that the significantly different expression of proteins may contribute to AS pathogenesis,which can be used as a mechanism of reference and supplement.