CAJ | 학술논문

目的探讨HLA-DQB1基因多态性与乙型肝炎原发性肝癌的相关性,寻找乙型肝炎原发性肝癌的易感基因及抗性基因. 方法收集山西医科大学第一医院的118例乙型肝炎相关性肝癌患者资料,根据有无乙型肝炎家族史及HBV DNA阴性或阳性各分为两组,采用聚合酶链反应-直接碱基序列分析基因分型(PCR-SBT)技术,检测该人群HLA-DQB1基因型.直接计算各等位基因频率,组间比较用x2检验或U检验,借助SPSS16.0统计软件进行数据分析.结果 HLA-DQB1 *0202、*0301等位基因频率肝癌组(11.8％、29.3％)高于正常人群组(7.6％、21.1％),差异有统计学意义(u值分别为2.43、3.09,P值均＜0.05),RR分别为1.581、1.477.HLA-DQB1*0202、*0301等位基因频率有乙型肝炎家族史组(14.1％、29.5％)高于正常人群组(7.6％、21.1％),差异有统计学意义(u值分别为3.76、3.16,P值均＜0.05),RR分别为1.928、1.495;HLA-DQB1*0301等位基因频率HBV DNA阳性组(35.0％)高于HBV DNA阴性组的频率(23.3％),而HLA-DQB1*0302等位基因频率HBV DNA阳性组(1.7％)低于HBV DNA阴性组的频率(6.9％),差异有统计学意义(x2=5.543,P＜ 0.05;x2=4.604,P＜0.05),RR分别为1.775、0.229. 结论 HLA-DQB1*0202、*0301等位基因可能是乙型肝炎相关性原发性肝癌同时也可能是家族性乙型肝炎原发性肝癌的易感基因,且HLA-DQB1*0301等位基因可能与HBV DNA复制有关,进而导致肝癌;HLA-DQB1*0302等位基因可能与抑制HBV DNA的复制有关,从而减少肝癌发生.
목적 탐토HLA-DQB1기인다태성여을형간염원발성간암적상관성,심조을형간염원발성간암적역감기인급항성기인. 방법 수집산서의과대학제일의원적118례을형간염상관성간암환자자료,근거유무을형간염가족사급HBV DNA음성혹양성각분위량조,채용취합매련반응-직접감기서렬분석기인분형(PCR-SBT)기술,검측해인군HLA-DQB1기인형.직접계산각등위기인빈솔,조간비교용x2검험혹U검험,차조SPSS16.0통계연건진행수거분석.결과 HLA-DQB1 *0202、*0301등위기인빈솔간암조(11.8％、29.3％)고우정상인군조(7.6％、21.1％),차이유통계학의의(u치분별위2.43、3.09,P치균＜0.05),RR분별위1.581、1.477.HLA-DQB1*0202、*0301등위기인빈솔유을형간염가족사조(14.1％、29.5％)고우정상인군조(7.6％、21.1％),차이유통계학의의(u치분별위3.76、3.16,P치균＜0.05),RR분별위1.928、1.495;HLA-DQB1*0301등위기인빈솔HBV DNA양성조(35.0％)고우HBV DNA음성조적빈솔(23.3％),이HLA-DQB1*0302등위기인빈솔HBV DNA양성조(1.7％)저우HBV DNA음성조적빈솔(6.9％),차이유통계학의의(x2=5.543,P＜ 0.05;x2=4.604,P＜0.05),RR분별위1.775、0.229. 결론 HLA-DQB1*0202、*0301등위기인가능시을형간염상관성원발성간암동시야가능시가족성을형간염원발성간암적역감기인,차HLA-DQB1*0301등위기인가능여HBV DNA복제유관,진이도치간암;HLA-DQB1*0302등위기인가능여억제HBV DNA적복제유관,종이감소간암발생.
Objective To investigate the correlation between polymorphisms in human leukocyte antigen (HLA)-DQB 1 and primary liver cancer (PLC) with hepatitis B virus (HBV) and to search for susceptibility and resistance genes related to PLC with HBV.Methods One hundred and eighteen patients with HBV-related liver cancer were enrolled from the First Hospital of Shanxi Medical University.Patients were stratified by family history of hepatitis B (39 with;79 without) and HBV DNA positivity (60 positive,≥ 1 × 103 IU/mL;58 negative,＜ 1 × 103 IU/mL).The HLA-DQB 1 genotype was determined by PCR and direct nucleotide sequence analysis genotyping.Allele frequencies were calculated by the direct counting method.Betweengroup comparisons were carried out with the Chi-square test or Mann-Whitney U test.Results The allele frequencies of HLA-DQBl*0202 and HLA-DQBl*0301 were significantly higher in patients with hepatocellular carcinoma (HCC) than the control group (1 1.8％ and 29.3％ vs.7.6％ and 21.1％;U =2.43 and 3.09,P ＜ 0.05,RR =1.581 and 1.477).The allele frequencies of HLA-DQB1*0202 and HLADQB 1*0301 were significantly higher in patients with HCC and familial history of hepatitis B than in the normal population (14.1％ and 29.5％ vs.7.6％ and 21.1％;U =3.76 and 3.16,P ＜ 0.05,RR =1.928 and 1.495).The allele frequency of HLA-DQB 1*0301 was significantly higher in the HBV DNA positive group than in the HBV DNA negative group (35.0％ vs.23.3％;x2 =5.543,P ＜ 0.05,RR=1.775),while the frequency of HLA-DQB1*0302 was significantly lower in the HBV DNA positive group than in the HBV DNA negative group (10.9％ vs.14.7％;x2 =4.604,P ＜ 0.05,RR =0.229).Conclusion The HLA-DQB 1 *0202 and HLA-DQB 1*0301 alleles may represent susceptibility for PLC with hepatitis B as well as for familial hepatitis B liver cancer.The HLA-DQB 1*0301 allele may support replication of HBV DNA,facilitating progression to liver cancer.The HLA-DQB1*0302 allele may inhibit replication of HBV DNA and reduce the incidence of liver cancer.