中外医疗
中外醫療
중외의료
CHINA FOREIGN MEDICAL TREATMENT
2015年
8期
192-193
,共2页
实时荧光定量逆转录-聚合酶链反应%细胞培养法%流感病毒
實時熒光定量逆轉錄-聚閤酶鏈反應%細胞培養法%流感病毒
실시형광정량역전록-취합매련반응%세포배양법%류감병독
Real-time quantitative reverse transcriotion-polymerase chain reaction(RT-PCR)%Cell culture%Flu virus
目的：比较实时荧光定量逆转录-聚合酶链反应(RT-PCR)法与细胞培养法检测流行性感冒病毒的差异。方法采用实时荧光定量RT-PCR及经典的狗肾传代细胞病毒分离2种方法,同时对流感监测点送检的80份疑似流感标本检测流感病毒。结果细胞培养病毒分离的阳性数为26份,实时荧光定量RT-PCR的阳性数为36份，χ2=8.1， P<0.005。结论通过该实验，验证了实时荧光定量RT-PCR的确快速敏感,适用于实验室快速诊断。
目的：比較實時熒光定量逆轉錄-聚閤酶鏈反應(RT-PCR)法與細胞培養法檢測流行性感冒病毒的差異。方法採用實時熒光定量RT-PCR及經典的狗腎傳代細胞病毒分離2種方法,同時對流感鑑測點送檢的80份疑似流感標本檢測流感病毒。結果細胞培養病毒分離的暘性數為26份,實時熒光定量RT-PCR的暘性數為36份，χ2=8.1， P<0.005。結論通過該實驗，驗證瞭實時熒光定量RT-PCR的確快速敏感,適用于實驗室快速診斷。
목적：비교실시형광정량역전록-취합매련반응(RT-PCR)법여세포배양법검측류행성감모병독적차이。방법채용실시형광정량RT-PCR급경전적구신전대세포병독분리2충방법,동시대류감감측점송검적80빈의사류감표본검측류감병독。결과세포배양병독분리적양성수위26빈,실시형광정량RT-PCR적양성수위36빈，χ2=8.1， P<0.005。결론통과해실험，험증료실시형광정량RT-PCR적학쾌속민감,괄용우실험실쾌속진단。
Objective Comparison the difference detection of influenza viruses between real-time quantitative reverse transcrio-tion-polymerase chain reaction (RT-PCR) and cell culture. Methods Using real-time quantitative RT-PCR and classic MDCK cells virus isolation two ways, censorship influenza surveillance point 80 copies of suspected flu specimens of influenza virus si-multaneously. Results Consequence The positive of virus isolation cells is 26 copies. The positive of real-time quantitative RT-PCR is 36 copies, χ2=8.1, P<0.005. Conclusion Through this experiment, verifying the real-time quantitative RT-PCR is really fast sensitive and suitable for rapid diagnostic laboratory.
