CAJ | 학술논문

目的测定不同缺铁程度足月孕妇血清铁调素的浓度及胎盘中膜铁转运蛋白1(FPN1)mRNA的表达水平,探讨其在母婴铁转运中的作用及调控机制.方法以55例足月孕妇为研究对象,检测母婴HGB、血清铁(SI)及血清铁蛋白(SF)水平.采用RT-PCR方法检测胎盘组织FPN1 mRNA的表达水平,应用双抗夹心生物素-亲和素-酶联免疫吸附试验检测血清铁调素浓度.比较不同铁状态孕妇FPN1 mRNA及铁调素浓度的差异.结果不同缺铁程度组孕妇相应新生儿脐血HGB、SI、SF水平比较差异均无统计学意义(P值均＞ 0.05).正常组、铁缺乏组及缺铁性贫血(IDA)组孕妇血清铁调素浓度分别为(193.637±52.219)、(176.523±43.875)及(147.623±37.768)μg/L,差异有统计学意义(F=3.872,P=0.027);胎盘FPN1 mRNA的表达水平分别为0.462±0.077、0.507±0.074及0.551±0.104,差异有统计学意义(F=4.767,P=0.013).孕妇血清铁调素水平与胎盘FPN1 mRNA表达水平呈负相关(r=-0.383,P=0.004).结论不同缺铁程度孕妇相应新生儿铁状态无明显差异,随着母体缺铁程度的加重,血清铁调素浓度下调,胎盘FPN1 mRNA表达上调.
목적 측정불동결철정도족월잉부혈청철조소적농도급태반중막철전운단백1(FPN1)mRNA적표체수평,탐토기재모영철전운중적작용급조공궤제.방법 이55례족월잉부위연구대상,검측모영HGB、혈청철(SI)급혈청철단백(SF)수평.채용RT-PCR방법검측태반조직FPN1 mRNA적표체수평,응용쌍항협심생물소-친화소-매련면역흡부시험검측혈청철조소농도.비교불동철상태잉부FPN1 mRNA급철조소농도적차이.결과 불동결철정도조잉부상응신생인제혈HGB、SI、SF수평비교차이균무통계학의의(P치균＞ 0.05).정상조、철결핍조급결철성빈혈(IDA)조잉부혈청철조소농도분별위(193.637±52.219)、(176.523±43.875)급(147.623±37.768)μg/L,차이유통계학의의(F=3.872,P=0.027);태반FPN1 mRNA적표체수평분별위0.462±0.077、0.507±0.074급0.551±0.104,차이유통계학의의(F=4.767,P=0.013).잉부혈청철조소수평여태반FPN1 mRNA표체수평정부상관(r=-0.383,P=0.004).결론 불동결철정도잉부상응신생인철상태무명현차이,수착모체결철정도적가중,혈청철조소농도하조,태반FPN1 mRNA표체상조.
Objective To detect the concentration of serum hepcidin and the mRNA expression level of ferroportinl (FPN1) in the placenta membrane from full term pregnant women with different degree of iron deficiency,and explore their roles for iron transport in placental.Methods The concentration of HGB,serum iron (SI) and serum ferritin (SF) of mothers and infants were detected in 55 full term pregnant women and neonates.The expression level of FPN1 mRNA in placental was detected by the RT-PCR technique.The concentration of serum hepcidin was detected by double antibody sandwich biotin avidin enzyme-linked immunosorbent assay.The serum hepcidin level and the FPN1 mRNA expression in the full term placenta from different maternal iron status were compared in three groups.Results There were no significant differences in the cord blood HGB,SI and SF of newborns from pregnant women with different iron status (P ＞ 0.05).The concentration of serum hepcidin of pregnant women among normal,iron deficiency and mild iron deficiency anemia were (193.637±52.219),(176.523± 43.875),and (147.623 ± 37.768) μg/L respectively,with statistical significance (F=3.872,P=0.027).The expression levels of FPN1 mRNA among three groups were 0.462±0.077,0.507±0.074 and 0.551±0.104 respectively,with statistical significance (F=4.767,P=0.013).A negative correlation between maternal serum hepcidin and placental FPN1 mRNA (r=-0.383,P=0.004) was identified.Conclusion There were no significant differences in the iron status of corresponding newborns from pregnant women with different iron status.With the severity of maternal iron deficiency,the concentration of serum hepcidin was down-regulated,while the expression of FPN1 mRNA in placenta was up-regulated.