CAJ | 학술논문

目的探讨深圳地区隐匿性HBV感染相关的HBV分子病毒学特征.方法从2010年4月至2012年12月深圳市血液中心采集的310 167份无偿献血者标本中筛查HBsAg阴性HBV DNA阳性标本,荧光定量PCR检测HBV DNA水平,套式PCR扩增HBV S区基因,分析隐匿性HBV感染相关HBV的分子生物学特征.使用MEGA软件以邻接法构建系统进化树进行基因分型.结果筛查出121份HBsAg阴性HBV DNA阳性标本,99份确认为隐匿性HBV感染,其中69份可分型的样品中B基因型57份,C基因型12份.在主要亲水区(MHR)氨基酸的变异中,B基因型隐匿性HBV感染毒株突变频率最高的是F134I/L,出现12次;C基因型MHR区域未出现明显的氨基酸变异.其他S区蛋白序列中,B基因型10株出现P105R、10株出现Q101R/H/K、9株出现G102I、V177A、9株出现S39N等氨基酸置换;C基因型10株出现S53L氨基酸置换.结论 S蛋白氨基酸的置换、插入、终止可能影响HBV的复制与蛋白的表达,与隐匿性HBV感染的发生和发展密切相关.
목적 탐토심수지구은닉성HBV감염상관적HBV분자병독학특정.방법 종2010년4월지2012년12월심수시혈액중심채집적310 167빈무상헌혈자표본중사사HBsAg음성HBV DNA양성표본,형광정량PCR검측HBV DNA수평,투식PCR확증HBV S구기인,분석은닉성HBV감염상관HBV적분자생물학특정.사용MEGA연건이린접법구건계통진화수진행기인분형.결과 사사출121빈HBsAg음성HBV DNA양성표본,99빈학인위은닉성HBV감염,기중69빈가분형적양품중B기인형57빈,C기인형12빈.재주요친수구(MHR)안기산적변이중,B기인형은닉성HBV감염독주돌변빈솔최고적시F134I/L,출현12차;C기인형MHR구역미출현명현적안기산변이.기타S구단백서렬중,B기인형10주출현P105R、10주출현Q101R/H/K、9주출현G102I、V177A、9주출현S39N등안기산치환;C기인형10주출현S53L안기산치환.결론 S단백안기산적치환、삽입、종지가능영향HBV적복제여단백적표체,여은닉성HBV감염적발생화발전밀절상관.
Objective To investigate the molecular virological characteristics of occult hepatitis B virus (HBV) infection (OBI) in Shenzhen.Methods A total of 310 167 blood samples were collected from donors between April 2010 and December 2012 in Shenzhen Blood Center.Hepatitis B surface antigen (HBsAg) /HBV DNA+ samples were screened.HBV DNA was quantified by quantitative polymerase chain reaction (PCR).S region was tested by nested PCR.Molecular virological characteristics of OBI samples were further analyzed.Genotyping was conducted by MEGA software to construct the phylogenetic tree by neighbor-joint.Results Among 121 HBsAg-/HBV DNA+ samples,99 samples were identified as OBI.Genotyping was conducted in 69 samples,with 57 of genotype B and 12 of genotype C.F134I/L mutation in 12 samples in the major hydrophilic region (MHR) of amino acids was the most frequent mutation in B genotype strains,and there was no obvious amino acid mutation in C genotype strains.In other S fragment sequences,10 of the B genotype OBI stains had P105R,10 had Q101R/H/K,9 had G102I,V177A and 9 had S39N amino acid replacements.While 10 of the C genotype OBI strains had S53L amino acid substitution.Conclusions Mutation,insertion and termination of amino acids in S protein may influence HBV replication and protein expression,which is related with the emergence and development of OBI.