临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2015年
5期
540-543,544
,共5页
尿毒症%急性冠状动脉综合征%激肽原,高分子量%中性粒细胞细胞外网络%接触系统
尿毒癥%急性冠狀動脈綜閤徵%激肽原,高分子量%中性粒細胞細胞外網絡%接觸繫統
뇨독증%급성관상동맥종합정%격태원,고분자량%중성립세포세포외망락%접촉계통
uremia%acute coronary syndrome%kininogen,high-molecular-weight%neutrophil extracellular traps%contact system
目的：探讨中性粒细胞细胞外网络(NETs)与接触系统在尿毒症急性冠状动脉综合征(ACS)中的作用。方法选择尿毒症患者44例,分为尿毒症 ACS 组[包括尿毒症 ACS 感染组(A1组)和尿毒症 ACS 非感染组(A2组)两个亚组]、尿毒症非 ACS 组[包括尿毒症非 ACS 感染组(B1组)和尿毒症非 ACS 非感染组(B2组)两个亚组],正常健康查体人群作为健康对照组。采用酶联免疫吸附测定(ELISA)试剂盒,分别检测血清中瓜氨酸化组蛋白 H3(CitH3)和高分子量激肽原(HK)的水平。结果①尿毒症 ACS 组、尿毒症非 ACS 组的血清 CitH3(229.35±131.51)μg/L、(175.40±91.72)μg/L 水平高于健康对照组(120.61±56.18)μg/L；HK(354.55±208.16)ng/L、(429.13±131.62)ng/L 低于健康对照组(562.81±138.80)ng/L,差异有统计学意义(均 P <0.05)。②与 B2组比较,A1组血清 CitH3水平增高(269.31±131.66)μg/L vs (142.65±56.69)μg/L；血清 HK 水平降低(272.84±158.44)ng/L vs (486.44±133.12)ng/L,差异有统计学意义(均 P <0.01)。③A1组中血清 CitH3水平与血小板呈负相关(r =-0.731,P <0.05)。结论 NETs 与接触系统激活在尿毒症患者 ACS 的发病过程中发挥重要作用。
目的：探討中性粒細胞細胞外網絡(NETs)與接觸繫統在尿毒癥急性冠狀動脈綜閤徵(ACS)中的作用。方法選擇尿毒癥患者44例,分為尿毒癥 ACS 組[包括尿毒癥 ACS 感染組(A1組)和尿毒癥 ACS 非感染組(A2組)兩箇亞組]、尿毒癥非 ACS 組[包括尿毒癥非 ACS 感染組(B1組)和尿毒癥非 ACS 非感染組(B2組)兩箇亞組],正常健康查體人群作為健康對照組。採用酶聯免疫吸附測定(ELISA)試劑盒,分彆檢測血清中瓜氨痠化組蛋白 H3(CitH3)和高分子量激肽原(HK)的水平。結果①尿毒癥 ACS 組、尿毒癥非 ACS 組的血清 CitH3(229.35±131.51)μg/L、(175.40±91.72)μg/L 水平高于健康對照組(120.61±56.18)μg/L；HK(354.55±208.16)ng/L、(429.13±131.62)ng/L 低于健康對照組(562.81±138.80)ng/L,差異有統計學意義(均 P <0.05)。②與 B2組比較,A1組血清 CitH3水平增高(269.31±131.66)μg/L vs (142.65±56.69)μg/L；血清 HK 水平降低(272.84±158.44)ng/L vs (486.44±133.12)ng/L,差異有統計學意義(均 P <0.01)。③A1組中血清 CitH3水平與血小闆呈負相關(r =-0.731,P <0.05)。結論 NETs 與接觸繫統激活在尿毒癥患者 ACS 的髮病過程中髮揮重要作用。
목적：탐토중성립세포세포외망락(NETs)여접촉계통재뇨독증급성관상동맥종합정(ACS)중적작용。방법선택뇨독증환자44례,분위뇨독증 ACS 조[포괄뇨독증 ACS 감염조(A1조)화뇨독증 ACS 비감염조(A2조)량개아조]、뇨독증비 ACS 조[포괄뇨독증비 ACS 감염조(B1조)화뇨독증비 ACS 비감염조(B2조)량개아조],정상건강사체인군작위건강대조조。채용매련면역흡부측정(ELISA)시제합,분별검측혈청중과안산화조단백 H3(CitH3)화고분자량격태원(HK)적수평。결과①뇨독증 ACS 조、뇨독증비 ACS 조적혈청 CitH3(229.35±131.51)μg/L、(175.40±91.72)μg/L 수평고우건강대조조(120.61±56.18)μg/L；HK(354.55±208.16)ng/L、(429.13±131.62)ng/L 저우건강대조조(562.81±138.80)ng/L,차이유통계학의의(균 P <0.05)。②여 B2조비교,A1조혈청 CitH3수평증고(269.31±131.66)μg/L vs (142.65±56.69)μg/L；혈청 HK 수평강저(272.84±158.44)ng/L vs (486.44±133.12)ng/L,차이유통계학의의(균 P <0.01)。③A1조중혈청 CitH3수평여혈소판정부상관(r =-0.731,P <0.05)。결론 NETs 여접촉계통격활재뇨독증환자 ACS 적발병과정중발휘중요작용。
Objective To investigate the mechanism of neutrophil extracellular traps(NETs)and contact system in uremic patients with acute coronary syndrome(ACS).Methods Uremic patients were divided into uremia ACS group (subdivided into uremia ACS infection group [group A1]and uremia ACS non-infection group [group A2])and uremia non-ACS group (subdivided into uremia non-ACS infection group[group B1]and uremia non-ACS non-infection group [group B2 ]),normal healthy patients were chosen as healthy control group.Serum citrullinated histone H3 (CitH3)and high molecular weight kininogen(HK)concentrations were detected with ELISA kits.Results ①Serum CitH3 levels in uremia ACS group (229.35±131.5 1)μg/L and uremia non-ACS group (1 75.40±91.72)μg/L were significantly higher than that of healthy control group (120.61±56.18)μg/L(P <0.05).Serum HK levels in uremia ACS group (354.55±208.1 6)ng/L and uremia non-ACS group (429.13±131.62)ng/L were significantly lower than that of healthy control group (562.81±138.80)ng/L(P <0.05).②Compared with group B2,serum CitH3 level of group A1 was significantly increased (269.31±131.66)μg/L vs (142.65±56.69)μg/L,respectively(P <0.01),and serum HK level of group A1 was significantly decreased (272.84 ± 1 58.44 )ng/L vs (486.44 ± 133.12 )ng/L, respectively(P < 0.01 ).③ Serum CitH3 level of group A1 showed a significant negative correlation with platelet account (r = -0.731,P < 0.05 ).Conclusion NETs and contact system activation played important roles in the mechanism of uremic patients with ACS.
