CAJ | 학술논문

目的：探讨烯酯酰辅酶A水解酶1(enoyl coenzyme A hydratase 1, Ech1)表达变化在肝癌中的意义及Ech1的亚细胞定位与肿瘤恶性生物学行为的关系。方法应用免疫组化法检测20例正常肝组织和30例原发性肝癌组织中Ech1的表达；应用亚细胞分离法检测Ech1在Hca-F和FEch1下调细胞的四种不同亚细胞组分中的表达。结果 Ech1在原发性肝癌中的表达高于正常肝组织,差异有统计学意义(P<0．05)；Ech1表达与患者年龄、AFP值及是否伴有肝硬化或伴有病毒性肝炎差异无统计学意义(P>0．05)。 Ech1在Hca-F和FEch1下调细胞的四种亚细胞组分中表达均定位于细胞质和细胞膜,并且细胞质表达强于细胞膜,细胞核和细胞骨架部分中不表达。结论 Ech1在肝癌组织中的表达明显高于正常肝组织,提示其可能与肝癌的发生、发展有关；体外培养细胞中Ech1表达调控对其亚细胞定位的改变没有影响,Ech1对肿瘤恶性生物学行为的影响可能不是通过其亚细胞定位改变而实现的。
목적：탐토희지선보매A수해매1(enoyl coenzyme A hydratase 1, Ech1)표체변화재간암중적의의급Ech1적아세포정위여종류악성생물학행위적관계。방법응용면역조화법검측20례정상간조직화30례원발성간암조직중Ech1적표체；응용아세포분리법검측Ech1재Hca-F화FEch1하조세포적사충불동아세포조분중적표체。결과 Ech1재원발성간암중적표체고우정상간조직,차이유통계학의의(P<0．05)；Ech1표체여환자년령、AFP치급시부반유간경화혹반유병독성간염차이무통계학의의(P>0．05)。 Ech1재Hca-F화FEch1하조세포적사충아세포조분중표체균정위우세포질화세포막,병차세포질표체강우세포막,세포핵화세포골가부분중불표체。결론 Ech1재간암조직중적표체명현고우정상간조직,제시기가능여간암적발생、발전유관；체외배양세포중Ech1표체조공대기아세포정위적개변몰유영향,Ech1대종류악성생물학행위적영향가능불시통과기아세포정위개변이실현적。
Purpose To study the expression of Ech1 in hepatocarcinoma and its clinical significance, and to explore the relationship between subcellular location of Ech1 and malignant biologic behaviour of hepatocarcinoma. Methods Immunohistochemical analysis was used to detect Ech1 expression in the 20 cases of normal liver tissue and the 30 cases of hepatocarcinoma. Subcellular location of Ech1 in Hca-F and FEch1-down cell was observed with subcellular protein extraction. Results The expression of Ech1 in primary hepa-tocarcinoma was increased compared to that of normal liver tissues ( P<0. 05 ) , while there was no significant difference between the expression of Ech1 with the age, AFP, and with or without liver cirrhosis or hepatitis virus in hepatocarcinoma (P>0. 05). Ech1 was found expressed almost at the same location although the expression level one is normal and the other is downregulation. Ech1 expres-sion was found in cytosol, membrane fraction, and its expression was higher in cytosol than other fractions both in Hca-F cell and Ech1 downregulated Hca-F cell. Conclusions The expression of Ech1 in primary hepatocarcinoma was increased, which may indicate that Ech1 is a critical factor in the development of primary hepatocarcinoma, but the subcellular location of Ech1 has not much contribution to that.