CAJ | 학술논문

采用水培的方法，研究了Mn、Zn单一及复合污染对锰超富集植物水蓼(Polygonum hydropiper L.)生长、氮代谢关键酶（谷氨酰胺合酶(GOGAT)、谷氨酸合成酶(GS)、硝酸还原酶(NR)、谷氨酸脱氢酶(GDH））活性以及硝态氮（NO3-）、铵态氮（NH4+）、可溶性蛋白质、游离脯氨酸、叶绿素a、叶绿素b和叶绿素a+b含量，水蓼对Mn、Zn的吸收和转移系数的影响。研究结果表明，水蓼根、茎、叶中Mn、Zn含量，随着Mn、Zn处理浓度的增加而增加，且水蓼对Zn和Mn的转移系数分别维持在1.0~1.8和1.0~11.9，表明Zn对水蓼体内Mn的转移影响较为明显；Mn/Zn单一及复合处理显著降低了水蓼叶绿素a、叶绿素b和叶绿素a+b的含量（P<0.05），但是叶绿素a/b较对照组显著提高（P<0.05），Mn/Zn复合处理抑制了根系生长及株重，T8时，根长仅为对照组的64.93%，而T9时，株重仅为对照组的65.45%；单Zn及Mn/Zn复合处理均降低了水蓼根系中NO3-的含量，以及抑制NR活性，T5时，叶片和根中NR活性分别是对照组的44.36%和34.41%，但提高了叶片与根系中NH4+的含量，T9时叶片中的NH4+含量是对照组的2.35倍，T8时根中的NH4+含量是对照组的1.58倍，以及增加了GS活性，T9时，叶和根中GS活性分别使对照组的1.32和1.57倍，Zn及Mn、Zn复合处理对水蓼叶片与根系中可溶性蛋白质含量无显著影响（P>0.05），表明GS活性以及GDH活性提高在消除NH4+胁迫过程中起重要作用。
채용수배적방법，연구료Mn、Zn단일급복합오염대맹초부집식물수료(Polygonum hydropiper L.)생장、담대사관건매（곡안선알합매(GOGAT)、곡안산합성매(GS)、초산환원매(NR)、곡안산탈경매(GDH））활성이급초태담（NO3-）、안태담（NH4+）、가용성단백질、유리포안산、협록소a、협록소b화협록소a+b함량，수료대Mn、Zn적흡수화전이계수적영향。연구결과표명，수료근、경、협중Mn、Zn함량，수착Mn、Zn처리농도적증가이증가，차수료대Zn화Mn적전이계수분별유지재1.0~1.8화1.0~11.9，표명Zn대수료체내Mn적전이영향교위명현；Mn/Zn단일급복합처리현저강저료수료협록소a、협록소b화협록소a+b적함량（P<0.05），단시협록소a/b교대조조현저제고（P<0.05），Mn/Zn복합처리억제료근계생장급주중，T8시，근장부위대조조적64.93%，이T9시，주중부위대조조적65.45%；단Zn급Mn/Zn복합처리균강저료수료근계중NO3-적함량，이급억제NR활성，T5시，협편화근중NR활성분별시대조조적44.36%화34.41%，단제고료협편여근계중NH4+적함량，T9시협편중적NH4+함량시대조조적2.35배，T8시근중적NH4+함량시대조조적1.58배，이급증가료GS활성，T9시，협화근중GS활성분별사대조조적1.32화1.57배，Zn급Mn、Zn복합처리대수료협편여근계중가용성단백질함량무현저영향（P>0.05），표명GS활성이급GDH활성제고재소제NH4+협박과정중기중요작용。
Hydroponic culture was conducted to study the effect of Mn and Zn combined single and pollution on the growth, heavy metals of Mn and Zn accumulation and transfer factor(TF), nitrogen metabolism in Mn-hyperaccumulatorPolygonum hydropiper L.. The enzymatic activies of nitrogen metabolism including nitrate reductase (NR), glutamine synthetase (GS), glutamineate synthase (GOGAT) glutamate dehydrogenase (GDH) as well as the concentrations of chlorophyll a, b a+b, a/b, NO3-, NH4+, free proline and soluble protein inPolygonum hydropiper L. was determined. Resalts showed that Mn and Zn concentrations of roots, stems and leaves of thePolygonum hydropiper L. , with the increase Mn and Zn control concentration increased,Polygonum hydropiper L. vary 1.0 from 11.9 of Mn and vary 1.0 from 1.8 of Zn of transfer factor. It indicated that Zn had affect on transfer of Mn. Mn/Zn treatment singinficantly reduced contents of chlorophyll a, b and a+b(P<0.05), but the chlorophyll a/b significantly increased than the control group (P<0.05). Mn/Zn treatment restrained growth of roots and biomass, when T8, root is only 64.93% and T9, biomass is only 65.45% of that in the control group. What single Zn and Mn/Zn combined pollution reduced the contents of NO3- and NR activities, when T5, NR activity in leaves and roots, respectively is 44.36% and 34.41% in the control group; but increased the contents of NH4+, T9, leaf of NH4+ content is 2.35 times that of the control group and T8 root of NH4+content is 1.58 times that of the control group; GS activities significantly increased than the control group (P<0.05) of roots and leaves, GS activities are 1.32 and 1.57 times that of the control group of leaf and ront when T9; had no singinficantly efected on soluble protein of roots and leaves ofPolygonum hydropiper L.. It indicated that play an important role in the process of elimination NH4+ stress.