CAJ | 학술논문

采用盐酸—间苯二酚溶液对酮糖的显色反应，探讨了显色反应机理与糖的结构关系，将正交试验法应用于显色条件的选择。通过将果糖、葡萄糖、蔗糖、乳糖与盐酸—间苯二酚试剂共热煮沸，在不同浓度、不同时间下观察实验现象，得出酮糖在浓度为1％左右、时间为2～5 min之间，反应最明显，而醛糖最不明显。可以用该试剂迅速地把酮糖和醛糖区别开来，测定结果令人满意。
채용염산—간분이분용액대동당적현색반응，탐토료현색반응궤리여당적결구관계，장정교시험법응용우현색조건적선택。통과장과당、포도당、자당、유당여염산—간분이분시제공열자비，재불동농도、불동시간하관찰실험현상，득출동당재농도위1％좌우、시간위2～5 min지간，반응최명현，이철당최불명현。가이용해시제신속지파동당화철당구별개래，측정결과령인만의。
Relationship between the mechanism of chromogenic reaction and the structure of carbohydrate was discussed and the orthogonal testes were applied to choose the chromogenic condition through the chromogenic reaction of hydrochloric acid -resor-cinol solution and ketoses .The ketoses and aldoses were quickly distinguished by observing the co -heating experiment of different carbohydrates at different concentration and time .The result was that the reaction phenomenon of the ketoses ( about 1%, t=2~5 min) were obvious , but aldoses were not .