CAJ | 학술논문

目的：研究脾氨肽口服冻干粉新的生物活性测定方法。方法：选用小鼠白细胞黏附抑制试验，通过对药物处理的不同浓度，粘附的不同时间，以及白细胞的不同浓度比较分析，检测3批脾氨肽口服冻干粉的体外生物活性。结果：经过比较，确定用2 mg? mL－1的药物处理浓度，黏附2 h，小鼠白细胞4×107个／mL用量，得到较好的结果。脾氨肽口服冻干粉与对照组相比，使未黏附白细胞显著增多（ P＜0．05），3批样品黏附抑制率均在20％以上。结论：白细胞黏附抑制试验可作为一种检测脾氨肽口服冻干粉体外生物活性的方法。
목적：연구비안태구복동간분신적생물활성측정방법。방법：선용소서백세포점부억제시험，통과대약물처리적불동농도，점부적불동시간，이급백세포적불동농도비교분석，검측3비비안태구복동간분적체외생물활성。결과：경과비교，학정용2 mg? mL－1적약물처리농도，점부2 h，소서백세포4×107개／mL용량，득도교호적결과。비안태구복동간분여대조조상비，사미점부백세포현저증다（ P＜0．05），3비양품점부억제솔균재20％이상。결론：백세포점부억제시험가작위일충검측비안태구복동간분체외생물활성적방법。
Objective:To study biological activity test for Spleen Aminopeptide Oral Lyophilized Powder .Methods:Comparing differ-ent drug doses, different adhesion times and different concentrations of leucocyte , a serial of conditions on leucocyte adhesion inhibition test were testified with spleen lymphocytes .Three batches of spleen aminopeptide were used in these leucocyte adhesion inhibition tests.Results:2 mg? mL-1 of drug dose with 4 ×10 7 cells? mL-1 of mouse spleen leucocyte in 2 hours of adhesion time were testified as a good condition to test spleen aminopeptide .The ratio of non-adhesion leucocyte increased significantly compared with the normal group ( P<0.05 ) and the leucocyte adhesion inhibition index ( LAI) achieved above 20%.Conclusion: Mouse leucocyte adhesion inhibition test can be used as a practical method to determine biological activity of Spleen Aminopeptide Oral Lyophilized Powder .