CAJ | 학술논문

目的：探讨microRNA-100(miR-100)在大鼠肝脏发育成熟和癌变时的表达。方法取新生SD乳鼠、成年SD大鼠肝组织以及经连续喂饲2-乙酰氨基芴加部分肝切除术(2-AAF/PH)诱导的SD大鼠肝癌组织,分别采用实时荧光定量PCR及FISH技术检测miR-100的表达。结果同新生SD乳鼠相比,miR-100在成年SD大鼠肝组织中表达明显升高( P<0．05)。大鼠肝脏癌变组织的miR-100表达较成年大鼠肝组织明显下降( P<0．05),接近于新生SD乳鼠miR-100的表达水平。结论 miR-100可作为SD大鼠肝脏发育成熟的标志,其表达降低可能与肿瘤形成有关。
목적：탐토microRNA-100(miR-100)재대서간장발육성숙화암변시적표체。방법취신생SD유서、성년SD대서간조직이급경련속위사2-을선안기물가부분간절제술(2-AAF/PH)유도적SD대서간암조직,분별채용실시형광정량PCR급FISH기술검측miR-100적표체。결과동신생SD유서상비,miR-100재성년SD대서간조직중표체명현승고( P<0．05)。대서간장암변조직적miR-100표체교성년대서간조직명현하강( P<0．05),접근우신생SD유서miR-100적표체수평。결론 miR-100가작위SD대서간장발육성숙적표지,기표체강저가능여종류형성유관。
Purpose To explore the effect of miR-100 in rat liver development and the occurrence of liver cancer. Methods The liv-er tissues of neonate SD rats, adult SD rats and 2-AAF/PH rats were collected, then real-time fluorescence quantitative polymerase chain reaction ( RT-PCR) and FISH methods were used to detect the expression of miR-100 in the liver tissues. Results The expres-sion level of miR-100 in adult SD rats liver tissues was significantly higher than that in neonate SD rats liver tissues (P<0. 05). Com-pared with the adult rats, the expression level of miR-100 in 2-AAF/PH rats liver tissues was obviously decline (P<0. 05). Conclu-sion miR-100 can as a maker of liver mature, and its deletion maybe related to the tumor formation.