CAJ | 학술논문

目的采用99Tcm-Duramycin进行动脉粥样硬化(AS)动物模型SPECT/CT显像,探讨该分子探针用于活体AS易损斑块(VP)中凋亡细胞检测的可行性.方法对16只新西兰大白兔采用完全随机分组方法分成假手术对照组(A组,4只)、稳定斑块组(B组,4只)和VP组(C组,8只).A组用普通饲料、B、C组用高脂饲料喂养,喂养2周后对动物进行股动脉分离假手术(A、B组)和腹主动脉球囊拉伤术(C组).分别于喂养第4、第8、第12周对3组按体质量自耳缘静脉注射99Tcm-Duramycin 74 MBq/kg,注射后0.5、1、2h行SPECT/CT显像;并于第4、8周末处死A、B组各1只、C组2只,12周末处死剩余所有动物,进行离体腹主动脉SPECT显像、病理学染色分析和原位末端标记法(TUNEL)细胞凋亡检测,并计算细胞凋亡指数(AI).采用单因素方差分析处理数据.结果活体SPECT/CT显像A组始终未见腹主动脉放射性分布,B组在第12周、C组在第8和第12周显像均可见腹主动脉放射性分布.C组第12周腹主动脉局部放射性异常浓聚,T/NT为3.40±0.22,明显高于A、B2组的T/NT (2.12±0.65、2.68±0.18,F=198.775,P＜0.05),与腹主动脉离体显像及病理染色结果一致.3组动物12周末腹主动脉片段TUNEL测得的细胞AI分别为(0±0.02)％、(5.3±1.97)％和(25.4±6.32)％,C组的AI明显高于A、B2组(F=70.260,P＜0.05).结论 99Tcm-Duramycin可无创性检测AS的VP中的凋亡细胞,可为评价AS的稳定性提供信息.
목적 채용99Tcm-Duramycin진행동맥죽양경화(AS)동물모형SPECT/CT현상,탐토해분자탐침용우활체AS역손반괴(VP)중조망세포검측적가행성.방법 대16지신서란대백토채용완전수궤분조방법분성가수술대조조(A조,4지)、은정반괴조(B조,4지)화VP조(C조,8지).A조용보통사료、B、C조용고지사료위양,위양2주후대동물진행고동맥분리가수술(A、B조)화복주동맥구낭랍상술(C조).분별우위양제4、제8、제12주대3조안체질량자이연정맥주사99Tcm-Duramycin 74 MBq/kg,주사후0.5、1、2h행SPECT/CT현상;병우제4、8주말처사A、B조각1지、C조2지,12주말처사잉여소유동물,진행리체복주동맥SPECT현상、병이학염색분석화원위말단표기법(TUNEL)세포조망검측,병계산세포조망지수(AI).채용단인소방차분석처리수거.결과 활체SPECT/CT현상A조시종미견복주동맥방사성분포,B조재제12주、C조재제8화제12주현상균가견복주동맥방사성분포.C조제12주복주동맥국부방사성이상농취,T/NT위3.40±0.22,명현고우A、B2조적T/NT (2.12±0.65、2.68±0.18,F=198.775,P＜0.05),여복주동맥리체현상급병리염색결과일치.3조동물12주말복주동맥편단TUNEL측득적세포AI분별위(0±0.02)％、(5.3±1.97)％화(25.4±6.32)％,C조적AI명현고우A、B2조(F=70.260,P＜0.05).결론 99Tcm-Duramycin가무창성검측AS적VP중적조망세포,가위평개AS적은정성제공신식.
Objective To investigate the value of 99Tcm-Duramycin SPCET/CT in the detection of vulnerable plaque (VP) in atherosclerotic (AS) rabbit models.Methods Sixteen New Zealand male rabbits were randomly divided into three groups:group A (sham-operated group,n =4),group B (stable plaque group,n =4) and group C (VP group,n =8).Group A was fed on normal diet,and the other 2groups were fed on cholesterol diet for 12 weeks.Femoral artery dissection sham-operation was done in group A and group B,while balloon-induced abdominal aorta wall injury was produced in group C after 2 weeks'feeding.Animals were injected with 99Tcm-Duramycin (74 MBq/kg) and then SPECT/CT imaging was performed at the end of 4,8,and 12 weeks,respectively.Abdominal aortas were explanted for ex vivo imaging and histological characterization of plaque.The apoptosis index (AI) was calculated.One-way analysis of variance was used to analyze data.Results There was no radioactive uptake by the abdominal aorta in each group at the end of 4 weeks and no uptake in group A and group B at the end of 8 weeks.There was slightly uptake radioactive uptake by the abdominal aorta in group B at the end of 12 weeks and in group C at the end of 8 weeks.There was intense uptake at the lesions of AS rabbits in group C at the end of 12 weeks,and the T/NT value significantly higher than that of the other two groups (3.40±0.22 vs 2.12±0.65,2.68±0.18,F=198.775,P＜0.05).The result was confirmed in the ex vivo imaging of the explanted aorta.The AI of group C was significantly higher than that of group A and B ((25.4±6.32) ％ vs (0±0.02)％,(5.3± 1.97)％,F=70.260,P＜0.05).Conclusions 99Tcm-Duramycin scimigraphy could identify the apoptosis of VP in the rabbit AS models.It is a promising non-invasive method to diagnose AS plaques.