广州医科大学学报
廣州醫科大學學報
엄주의과대학학보
Academic Journal of Guangzhou Medical College
2014年
6期
1-5
,共5页
邓穗燕%李琴%林丽英%黄俊
鄧穗燕%李琴%林麗英%黃俊
산수연%리금%림려영%황준
急性冠脉综合征%Foxp3%调节性T细胞%IL-10%TGF-β1
急性冠脈綜閤徵%Foxp3%調節性T細胞%IL-10%TGF-β1
급성관맥종합정%Foxp3%조절성T세포%IL-10%TGF-β1
acute coronary syndrome%Foxp3%regulatory T cell%IL￣10%TGF￣β1
目的：：探讨急性冠脉综合征( ACS)患者外周血CD4＋CD25＋Foxp3＋Treg细胞亚群的表达量及其与ACS的关系。方法：采用流式细胞仪( FCM)检测16例ACS患者( ACS组)、12例稳定性心绞痛患者( SA组)和20例健康志愿者( Control组)外周血CD4＋CD25＋Tre细胞和CD4＋CD25＋Foxp3＋Treg细胞的百分含量；采用酶联免疫吸附试验( ELISA)检测3组研究对象外周血细胞因子TGF￣β1和IL￣10水平；并采用透射比浊法检测3组研究对象血浆超敏C￣反应蛋白( hs￣CRP )和血糖、血脂水平。结果：3组研究对象外周血CD4＋CD25＋Treg细胞百分含量差异无统计学意义( P>0.05)；ACS组外周血CD4＋CD25＋Foxp3＋Treg细胞水平和血浆TGF￣β1、IL￣10浓度较SA组和Control组均明显降低(P<0.05)，而血浆hs￣CRP则显著升高(P<0.05)；SA组与Control组相比，以上指标差异均无统计学意义(P>0.05)。结论：CD4＋CD25＋Foxp3＋Treg细胞数量和/或功能的下降可能与ACS的发生和发展密切相关，Foxp3标记可能有助于进一步识别ACS相关的Treg细胞。
目的：：探討急性冠脈綜閤徵( ACS)患者外週血CD4＋CD25＋Foxp3＋Treg細胞亞群的錶達量及其與ACS的關繫。方法：採用流式細胞儀( FCM)檢測16例ACS患者( ACS組)、12例穩定性心絞痛患者( SA組)和20例健康誌願者( Control組)外週血CD4＋CD25＋Tre細胞和CD4＋CD25＋Foxp3＋Treg細胞的百分含量；採用酶聯免疫吸附試驗( ELISA)檢測3組研究對象外週血細胞因子TGF￣β1和IL￣10水平；併採用透射比濁法檢測3組研究對象血漿超敏C￣反應蛋白( hs￣CRP )和血糖、血脂水平。結果：3組研究對象外週血CD4＋CD25＋Treg細胞百分含量差異無統計學意義( P>0.05)；ACS組外週血CD4＋CD25＋Foxp3＋Treg細胞水平和血漿TGF￣β1、IL￣10濃度較SA組和Control組均明顯降低(P<0.05)，而血漿hs￣CRP則顯著升高(P<0.05)；SA組與Control組相比，以上指標差異均無統計學意義(P>0.05)。結論：CD4＋CD25＋Foxp3＋Treg細胞數量和/或功能的下降可能與ACS的髮生和髮展密切相關，Foxp3標記可能有助于進一步識彆ACS相關的Treg細胞。
목적：：탐토급성관맥종합정( ACS)환자외주혈CD4＋CD25＋Foxp3＋Treg세포아군적표체량급기여ACS적관계。방법：채용류식세포의( FCM)검측16례ACS환자( ACS조)、12례은정성심교통환자( SA조)화20례건강지원자( Control조)외주혈CD4＋CD25＋Tre세포화CD4＋CD25＋Foxp3＋Treg세포적백분함량；채용매련면역흡부시험( ELISA)검측3조연구대상외주혈세포인자TGF￣β1화IL￣10수평；병채용투사비탁법검측3조연구대상혈장초민C￣반응단백( hs￣CRP )화혈당、혈지수평。결과：3조연구대상외주혈CD4＋CD25＋Treg세포백분함량차이무통계학의의( P>0.05)；ACS조외주혈CD4＋CD25＋Foxp3＋Treg세포수평화혈장TGF￣β1、IL￣10농도교SA조화Control조균명현강저(P<0.05)，이혈장hs￣CRP칙현저승고(P<0.05)；SA조여Control조상비，이상지표차이균무통계학의의(P>0.05)。결론：CD4＋CD25＋Foxp3＋Treg세포수량화/혹공능적하강가능여ACS적발생화발전밀절상관，Foxp3표기가능유조우진일보식별ACS상관적Treg세포。
Objective:To analyze the expression level of CD4+CD25+Foxp3+regulatory T ( Treg) cell subset in peripheral blood of patients with acute coronary syndrome ( ACS) and its correlation with ACS. Methods:Flow cytometry ( FCM) was used to measure the percentage contents of CD4+CD25+ Treg cells and CD4+CD25+Foxp3+Treg cells in the peripheral blood from 16 ACS patients ( ACS group) , 12 patients with stable angina ( SA group) , and 20 healthy volunteers ( Control group) . The levels of peripheral blood cytokines TGF￣β1 and IL￣10 in the three groups were measured by enzyme￣linked immunosorbent assay ( ELISA) . The levels of hypersensitive C￣reactive protein ( hs￣CRP ) , blood glucose and blood lipids were measured by transmission turbidimetry. Results:There was no statistical difference in the percentage content of peripheral blood CD4+CD25+Treg cells among the three groups ( P>0. 05 ) . The levels of peripheral CD4+ CD25+ Foxp3+ Treg cells and plasma concentrations of TGF￣β1 and IL￣10 in the ACS group were significantly lowered (P<0.05) while the level of plasma was significantly elevated (P<0.05) compared with those in the SA group and the control group. There was no statistically significant difference in these indexes between the SA group and the control group ( P>0.05) . Conclusion:The decline in counts and/or function of CD4+CD25+Foxp3+Treg cells may be associated with the occurrence and development of ACS. Foxp3 as a biomarker may favor further recognition of ACS￣related Treg cells.
