CAJ | 학술논문

目的：探讨微小RNA101（miR-101）在结直肠癌中的表达及其临床意义。方法采用锁定核苷酸原位杂交（LNA-ISH）技术检测组织芯片（56例正常黏膜、51例腺瘤和735例结直肠癌组织）中miR-101的表达水平，并分析其与患者临床病理特征及预后的关系。再用茎环RT-PCR法检测miR-101在5例正常黏膜、5例腺瘤和47例结直肠癌新鲜组织中的表达水平，验证LNA-ISH结果的准确性。结果从正常黏膜、腺瘤到Ⅰ期结直肠癌，miR-101的表达水平逐渐上升；从Ⅱ期、Ⅲ期到Ⅳ期结直肠癌，miR-101的表达水平逐渐下降（P＜0．01）。 miR-101高表达患者的淋巴结转移率低、远处转移率低、分化程度高及复发率低（均P＜0．01）。多因素预后分析显示，miR-101表达可独立预测结直肠癌患者的总生存率（HR=0.550，95% CI：0.351~0.863）和无瘤生存率（HR=0.562，95% CI：0.397~0.794），miR-101高表达提示患者预后较好。结论 miR-101与结直肠癌的发生发展有关，是预后的独立预测因素。
목적：탐토미소RNA101（miR-101）재결직장암중적표체급기림상의의。방법채용쇄정핵감산원위잡교（LNA-ISH）기술검측조직심편（56례정상점막、51례선류화735례결직장암조직）중miR-101적표체수평，병분석기여환자림상병리특정급예후적관계。재용경배RT-PCR법검측miR-101재5례정상점막、5례선류화47례결직장암신선조직중적표체수평，험증LNA-ISH결과적준학성。결과종정상점막、선류도Ⅰ기결직장암，miR-101적표체수평축점상승；종Ⅱ기、Ⅲ기도Ⅳ기결직장암，miR-101적표체수평축점하강（P＜0．01）。 miR-101고표체환자적림파결전이솔저、원처전이솔저、분화정도고급복발솔저（균P＜0．01）。다인소예후분석현시，miR-101표체가독립예측결직장암환자적총생존솔（HR=0.550，95% CI：0.351~0.863）화무류생존솔（HR=0.562，95% CI：0.397~0.794），miR-101고표체제시환자예후교호。결론 miR-101여결직장암적발생발전유관，시예후적독립예측인소。
Objective To investigate the microRNA101 (miR101) expression and its clinical significance in colorectal cancer. Methods Tissue microarrays containing 56 specimens of normal mucosa, 51 adenoma and 735 colorectal cancer were examined by locked nucleic acid in-situ hybridization (LNA-ISH) for miR101 expression. Relationship between miR101 expression and clinicopathologic parameters and prognosis of colorectal cancer patients were analyzed. Fresh frozen tissues containing 5 specimens of normal mucosa , 5 adenoma and 47 colorectal cancer were examined by RT-PCR to verify the accuracy of LNA-ISH. Results Expression of miR101 increased gradually from normal mucosa, adenoma to stage I colorectal cancer (all P<0.01), and decreased gradually from stageⅡ, stage Ⅲ to stage Ⅳ colorectal cancer (all P<0.01). Overexpression of miR101 was related with lower incidence of lymph node metastasis, lower metastasis rate, higher differentiation and lower recurrence rate (all P<0.01). Multivariate survival analysis demonstrated that miR101 expression was an independent prognostic factor of overall survival (HR=0.550, 95% CI: 0.351-0.863) and disease free survival (HR=0.562, 95% CI: 0.397-0.794) in colorectal cancer patients. Overexpression of miR101 predicted a better prognosis in colorectal cancer patients. Conclusions Expression of miR101 is associated with the genesis and development of colorectal cancer , and may serve as an independent prognostic factor for colorectal cancer patients.