CAJ | 학술논문

目的：探讨香菇多糖对细菌、真菌和病毒的抑制作用。方法细菌（溶血性链球菌、金黄色葡糖球菌、鼠伤寒沙门氏菌、枯草芽孢杆菌、大肠杆菌、多杀性巴氏杆菌、痢疾志贺氏菌、伤寒杆菌、甲型副伤寒杆菌）和真菌（白色念珠菌、啤酒酵母、红色毛癣菌、青霉、绿色木霉、黑根霉、烟曲霉）做成含菌平板，放置含有一定浓度香菇多糖的滤纸片，培养后通过测量抑菌圈计算最低抑菌浓度。对病毒（流感病毒、呼吸道合胞病毒、腺病毒、柯萨奇病毒A、单纯疱疹病毒1型、轮状病毒、埃可病毒2型）的抑制作用通过多糖先和细胞作用及先和病毒作用两种方式。结果香菇多糖对9种供试细菌和7种病毒都有抑制作用，只对7种供试真菌中的3种有微弱抑制作用。结论香菇多糖对细菌和病毒具有普遍抑制作用，而对真菌无普遍抑制作用。
목적：탐토향고다당대세균、진균화병독적억제작용。방법세균（용혈성련구균、금황색포당구균、서상한사문씨균、고초아포간균、대장간균、다살성파씨간균、이질지하씨균、상한간균、갑형부상한간균）화진균（백색념주균、비주효모、홍색모선균、청매、록색목매、흑근매、연곡매）주성함균평판，방치함유일정농도향고다당적려지편，배양후통과측량억균권계산최저억균농도。대병독（류감병독、호흡도합포병독、선병독、가살기병독A、단순포진병독1형、륜상병독、애가병독2형）적억제작용통과다당선화세포작용급선화병독작용량충방식。결과향고다당대9충공시세균화7충병독도유억제작용，지대7충공시진균중적3충유미약억제작용。결론향고다당대세균화병독구유보편억제작용，이대진균무보편억제작용。
Objective To investigate the general inhibitive effect of lentinan on bacteria,fungi and viruses. Methods Double layer agar plates were cultured with the following bacterias:Hemolytic streptococcus,Staphylococcus aureus glucose, Salmonella typhimurium,Bacillus subtilis,E. Coli,Pasteurella multocida,Shigella dysentery,Salmonella typhi,Paratyphoid bacilli and following fungi:Candida albicans,Brewer's yeast,Trichophyton rubrum,Penicillium,Viride,Rhizopus nigricans,As-pergillus fumigates. Filter paper with known concentration lentinan was put on top of the agar plates. After culture for 24 hours the minimal concentration of polysaccharide which inhibits bacterial/fungal growth was calculated by measurement of the size of inhibitive ring. The inhibition on viruses( influenza virus,respiratory syncytial virus,adenovirus,coxsackie virus A,herpes simplex virus-1,rotavirus,ECHO virus-2)was investigated by binding of lentinan to either the host cells or di-rectly to viruses. Results Lentinan showed inhibitive effect on all nine bacteria and seven viruses tested,but only slightly inhibited three out of seven fungi tested. Conclusion Lentinan had general inhibitive effect on bacteria and viruses but not on fungi.