CAJ | 학술논문

目的：探讨ABCG5和ABCG8基因在胆囊胆固醇结石和胆固醇息肉发生、发展中的作用。方法回顾性研究2012年3月至2014年3月在中山大学附属第一医院行胆囊切除术的60例患者临床资料。其中男31例，女29例；平均年龄（49±6）岁。所有患者均签署知情同意书，符合医学伦理学规定。根据病理学诊断结果，将患者分为胆囊胆固醇结石组(结石组)、胆囊胆固醇息肉组(息肉组)及正常胆囊切除对照组(对照组)。采用实时荧光定量PCR检测胆囊上皮细胞ABCG5和ABCG8 mRNA相对表达量。3组患者mRNA相对表达量的比较采用单因素方差分析和LSD-t检验。结果结石组ABCG5和ABCG8 mRNA相对表达量分别为3.3±0.9和6.9±1.5，明显高于对照组的2.4±0.6和4.3±1.5（LSD-t=23.58，16.55；P<0.05）。息肉组ABCG5和ABCG8 mRNA相对表达量分别为2.6±0.7和4.6±1.3，与对照组比较差异无统计学意义（LSD-t=1.18，0.73；P>0.05）。结论 ABCG5和ABCG8基因可能通过不同机制在胆囊胆固醇结石和胆固醇息肉的发生、发展中发挥作用。
목적：탐토ABCG5화ABCG8기인재담낭담고순결석화담고순식육발생、발전중적작용。방법회고성연구2012년3월지2014년3월재중산대학부속제일의원행담낭절제술적60례환자림상자료。기중남31례，녀29례；평균년령（49±6）세。소유환자균첨서지정동의서，부합의학윤리학규정。근거병이학진단결과，장환자분위담낭담고순결석조(결석조)、담낭담고순식육조(식육조)급정상담낭절제대조조(대조조)。채용실시형광정량PCR검측담낭상피세포ABCG5화ABCG8 mRNA상대표체량。3조환자mRNA상대표체량적비교채용단인소방차분석화LSD-t검험。결과결석조ABCG5화ABCG8 mRNA상대표체량분별위3.3±0.9화6.9±1.5，명현고우대조조적2.4±0.6화4.3±1.5（LSD-t=23.58，16.55；P<0.05）。식육조ABCG5화ABCG8 mRNA상대표체량분별위2.6±0.7화4.6±1.3，여대조조비교차이무통계학의의（LSD-t=1.18，0.73；P>0.05）。결론 ABCG5화ABCG8기인가능통과불동궤제재담낭담고순결석화담고순식육적발생、발전중발휘작용。
ObjectiveTo investigate ABCG5 and ABCG8 gene in the occurrence and development of cholesterol gallstone and cholesterol polyp.MethodsClinical data of 60 patients who underwent cholecystectomy in the First Afifliated Hospital of Sun Yat-sen University from March 2012 to March 2014 were studied retrospectively. Of the 60 patients, 31 were males and 29 were females with a mean age of (49±6) years old. The informed consents of all patients were obtained and the local ethical committee approval had been received. According to the results of pathological diagnosis, the patients were divided into the cholesterol gallstone group (gallstone group), the cholesterol polyp group (polyp group) and the routine cholecystectomy group (control group). The relative expression of ABCG5 and ABCG8 mRNA in gallbladder epithelial cells was determined by real-time fluorescence quantitative polymerase chain reaction (PCR). The comparison of the relative mRNA expression between three groups were conducted using One-way analysis of variance and LSD-t test.ResultsThe relative expression of ABCG5 and ABCG8 mRNA was 3.3±0.9 and 6.9±1.5 respectively in gallstone group, which were signiifcantly higher than 2.4±0.6 and 4.3±1.5 in control group (LSD-t=23.58, 16.55;P<0.05). The relative expression of ABCG5 and ABCG8 mRNA was 2.6±0.7 and 4.6±1.3 respectively in polyp group and no signiifcant difference was observed when comparing with those in control group (LSD-t=1.18, 0.73;P>0.05).ConclusionABCG5 and ABCG8 gene may play a role in the occurrence and development of cholesterol gallstone and cholesterol polyp through different mechanisms.